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Prognostic phenotypic classification for canine mammary tumors

  • Authors:
    • Giovanna Rossi Varallo
    • Gabriela Bottaro Gelaleti
    • Larissa Bazela Maschio‑Signorini
    • Marina Gobbe Moschetta
    • Juliana Ramos Lopes
    • Andrigo Barboza De Nardi
    • Mirela Tinucci‑Costa
    • Rafael Malagoli Rocha
    • Debora Aparecida Pires de Campos Zuccari
  • View Affiliations / Copyright

    Affiliations: Faculty of Agricultural and Veterinary Sciences, Universidade Estadual Paulista, Jaboticabal, São Paulo 14884‑900, Brazil, Institute of Biosciences, Letters and Exact Sciences, Universidade Estadual Paulista, São José do Rio Preto, São Paulo 15054‑000, Brazil, Laboratory of Molecular Investigation of Cancer, Department of Molecular Biology, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, São Paulo 15090‑000, Brazil, International Research Center‑A.C. Camargo Cancer Center, São Paulo, São Paulo 01508‑010, Brazil
    Copyright: © Varallo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6545-6553
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    Published online on: November 5, 2019
       https://doi.org/10.3892/ol.2019.11052
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Abstract

Mammary neoplasms are a heterogeneous form of disease, and in order to determine its course and biological features with more accuracy, investigations based on tumor phenotypes are required. The aim of the present study was to propose and validate a phenotypic classification for canine mammary tumors and to assess any association between clinicopathological characteristics, survival and prognosis. For the immunohistochemistry analysis, the primary antibodies against estrogen receptor‑α, progesterone receptor, human epidermal growth factor receptor 2 (HER‑2)/neu and E‑cadherin were used. A total of 110 canine mammary tumors were investigated; 42 tumors were classified as luminal A, 41 as luminal B, 17 as triple‑negative and 10 as HER‑2‑positive. The luminal A and B phenotypes were associated with improved prognosis, whereas HER‑2positive and triple‑negative tumors were more aggressive, and exhibited a significant association with the occurrence of metastasis, a worse Tumor‑Node‑Metastasis classification and shorter survival time (P<0.05). In addition, there were different levels of E‑cadherin expression intensity observed among the four tumor profiles investigated. Luminal A and B phenotypes presented an upregulation of E‑cadherin compared with the HER‑2 positive and triple‑negative phenotypes (P<0.05). From the results of the present study, the proposed immunohistochemical panel and phenotypic classification techniques could be useful diagnostic tools with a good technical applicability in veterinary oncology. The analysis of E‑cadherin expression in the panel of tumor markers allowed a more accurate classification for determining the biological features of the mammary tumor.
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Varallo GR, Gelaleti GB, Maschio‑Signorini LB, Moschetta MG, Lopes JR, De Nardi AB, Tinucci‑Costa M, Rocha RM and de Campos Zuccari DA: Prognostic phenotypic classification for canine mammary tumors. Oncol Lett 18: 6545-6553, 2019.
APA
Varallo, G.R., Gelaleti, G.B., Maschio‑Signorini, L.B., Moschetta, M.G., Lopes, J.R., De Nardi, A.B. ... de Campos Zuccari, D.A. (2019). Prognostic phenotypic classification for canine mammary tumors. Oncology Letters, 18, 6545-6553. https://doi.org/10.3892/ol.2019.11052
MLA
Varallo, G. R., Gelaleti, G. B., Maschio‑Signorini, L. B., Moschetta, M. G., Lopes, J. R., De Nardi, A. B., Tinucci‑Costa, M., Rocha, R. M., de Campos Zuccari, D. A."Prognostic phenotypic classification for canine mammary tumors". Oncology Letters 18.6 (2019): 6545-6553.
Chicago
Varallo, G. R., Gelaleti, G. B., Maschio‑Signorini, L. B., Moschetta, M. G., Lopes, J. R., De Nardi, A. B., Tinucci‑Costa, M., Rocha, R. M., de Campos Zuccari, D. A."Prognostic phenotypic classification for canine mammary tumors". Oncology Letters 18, no. 6 (2019): 6545-6553. https://doi.org/10.3892/ol.2019.11052
Copy and paste a formatted citation
x
Spandidos Publications style
Varallo GR, Gelaleti GB, Maschio‑Signorini LB, Moschetta MG, Lopes JR, De Nardi AB, Tinucci‑Costa M, Rocha RM and de Campos Zuccari DA: Prognostic phenotypic classification for canine mammary tumors. Oncol Lett 18: 6545-6553, 2019.
APA
Varallo, G.R., Gelaleti, G.B., Maschio‑Signorini, L.B., Moschetta, M.G., Lopes, J.R., De Nardi, A.B. ... de Campos Zuccari, D.A. (2019). Prognostic phenotypic classification for canine mammary tumors. Oncology Letters, 18, 6545-6553. https://doi.org/10.3892/ol.2019.11052
MLA
Varallo, G. R., Gelaleti, G. B., Maschio‑Signorini, L. B., Moschetta, M. G., Lopes, J. R., De Nardi, A. B., Tinucci‑Costa, M., Rocha, R. M., de Campos Zuccari, D. A."Prognostic phenotypic classification for canine mammary tumors". Oncology Letters 18.6 (2019): 6545-6553.
Chicago
Varallo, G. R., Gelaleti, G. B., Maschio‑Signorini, L. B., Moschetta, M. G., Lopes, J. R., De Nardi, A. B., Tinucci‑Costa, M., Rocha, R. M., de Campos Zuccari, D. A."Prognostic phenotypic classification for canine mammary tumors". Oncology Letters 18, no. 6 (2019): 6545-6553. https://doi.org/10.3892/ol.2019.11052
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