Colorectal cancer cells differentially impact migration and microRNA expression in endothelial cells

Kim,Do  Yei; Lee,Sang‑Soo; Bae,Young‑Kyung

doi:10.3892/ol.2019.11055

Colorectal cancer cells differentially impact migration and microRNA expression in endothelial cells

Authors:
- Do Yei Kim
- Sang‑Soo Lee
- Young‑Kyung Bae
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Affiliations: Center for Bioanalysis, Division of Chemical and Medical Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea
Published online on: November 5, 2019 https://doi.org/10.3892/ol.2019.11055
Pages: 6361-6370
Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Angiogenesis is an essential step in cancer progression and metastasis. Changes in the microRNA (miRNA or miR) expression profiles of endothelial cells (ECs) elicited by cancer cells promote angiogenesis. Vascular endothelial growth factor (VEGF), a key pro‑angiogenic factor, influences miRNA expression in ECs; however, the exact role that VEGF serves in miRNA regulation during angiogenesis is poorly defined. The present study aimed to demonstrate the differential angiogenic effects on human umbilical vein endothelial cells (HUVECs) of five different colorectal cancer (CRC) cell lines by in vitro HUVEC migration and angiogenesis assays in response to CRC‑conditioned medium (CM). Among the tested CMs, LoVo was the most effective cell line in eliciting HUVEC angiogenic phenotypes, at least partially due to its high VEGF level. It was also observed that pro‑angiogenesis‑regulatory miRNAs (angio‑miRNA) miR‑296, miR‑132, miR‑105 and miR‑200 were upregulated in the VEGF‑rich LoVo CM compared with the VEGF‑scarce SW620 CM. In addition, treatment with VEGF receptor 2 inhibitor downregulated the pro‑angio‑miRNAs, with the exception of miR‑132, suggesting that VEGF, as well as additional signaling, is required for angio‑miRNA expression. Quantitative analyses on pro‑angio‑miRNA target expression suggested that independent pathways may be involved in the regulation of their expression. Overall, the data from the present study indicated that multiple paracrine factors, including VEGF secreted by CRCs, effectively modulated angio‑miRNA expression, thus impacting their target expression and the angiogenic phenotypes of HUVECs.

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