Open Access

Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy in adrenocortical carcinoma cells

  • Authors:
    • Xuemei Huang
    • Lili Wu
    • Yaqi Kuang
    • Xin Li
    • Xiujun Deng
    • Xinghuan Liang
    • Li Li
    • Haiyan Yang
    • Zhenxing Huang
    • Decheng Lu
    • Zuojie Luo
  • View Affiliations

  • Published online on: November 5, 2019     https://doi.org/10.3892/ol.2019.11057
  • Pages: 6475-6482
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Adrenocortical carcinoma (ACC) is an invasive tumor that occurs in the endocrine system. Increasing evidence has shown that endoplasmic reticulum (ER) stress and autophagy play an important role in tumor formation. Tauroursodeoxycholic acid (TUDCA) is an ER chemical chaperone that can alleviate ER stress. In the present study, TUDCA promoted the proliferation, migration and invasion of ACC SW‑13 and NCI‑H295R cells. Reverse transcription‑quantitative PCR and western blot analysis showed that the expression of glucose‑regulated protein 78, a promoter of ER stress, was decreased. The expression levels of protein kinase R (PKR)‑like ER kinase and activating transcription factor 6 were correspondingly decreased, and the downstream proteins, C/EBP homologous protein and JNK, were also decreased. The expression levels of the autophagy factor microtubule‑associated protein light chain 3‑II/I and the anti‑apoptotic factor Bcl‑2 increased following TUDCA treatment, while the expression of the pro‑apoptotic factor Bax decreased. TUDCA alleviated ER stress in ACC SW‑13 and NCI‑H295R cells and induced autophagy, thereby inhibiting ACC cell apoptosis. ER stress‑ and autophagy‑related signaling pathways are involved in the occurrence of ACC, which may provide potential therapeutic targets for ACC treatment.
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December-2019
Volume 18 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Huang X, Wu L, Kuang Y, Li X, Deng X, Liang X, Li L, Yang H, Huang Z, Lu D, Lu D, et al: Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy in adrenocortical carcinoma cells. Oncol Lett 18: 6475-6482, 2019
APA
Huang, X., Wu, L., Kuang, Y., Li, X., Deng, X., Liang, X. ... Luo, Z. (2019). Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy in adrenocortical carcinoma cells. Oncology Letters, 18, 6475-6482. https://doi.org/10.3892/ol.2019.11057
MLA
Huang, X., Wu, L., Kuang, Y., Li, X., Deng, X., Liang, X., Li, L., Yang, H., Huang, Z., Lu, D., Luo, Z."Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy in adrenocortical carcinoma cells". Oncology Letters 18.6 (2019): 6475-6482.
Chicago
Huang, X., Wu, L., Kuang, Y., Li, X., Deng, X., Liang, X., Li, L., Yang, H., Huang, Z., Lu, D., Luo, Z."Tauroursodeoxycholic acid mediates endoplasmic reticulum stress and autophagy in adrenocortical carcinoma cells". Oncology Letters 18, no. 6 (2019): 6475-6482. https://doi.org/10.3892/ol.2019.11057