Upregulation of Holliday junction recognition protein predicts poor prognosis and biochemical recurrence in patients with prostate cancer

Chen,Yan‑Fei; Liang,Yu‑Xiang; Yang,Jian‑An; Yuan,Dao‑Zhang; Li,Jing; Zheng,Shun‑Sheng; Wan,Yue‑Ping; Wang,Bin; Han,Zhao‑Dong; Zhong,Wei‑De

doi:10.3892/ol.2019.11061

Upregulation of Holliday junction recognition protein predicts poor prognosis and biochemical recurrence in patients with prostate cancer

Authors:
- Yan‑Fei Chen
- Yu‑Xiang Liang
- Jian‑An Yang
- Dao‑Zhang Yuan
- Jing Li
- Shun‑Sheng Zheng
- Yue‑Ping Wan
- Bin Wang
- Zhao‑Dong Han
- Wei‑De Zhong
View Affiliations

Affiliations: Department of Urology, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, Guangdong 510095, P.R. China, Department of Urology, Guangdong Key Laboratory of Clinical Molecular Medicine and Diagnostics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China , Department of Urology, Huadu District People's Hospital, Southern Medical University, Guangzhou, Guangdong 510180, P.R. China
Published online on: November 5, 2019 https://doi.org/10.3892/ol.2019.11061
Pages: 6697-6703
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Abnormal expression of Holliday junction recognition protein (HJURP) in several types of tumor cells plays a vital role in the formation and progression of tumors. Few studies have investigated the role of HJURP in prostate cancer (PCa). The aim of this study was to analyze the expression levels of HJURP in PCa and to establish the association with clinicopathological data. Reverse transcription quantitative polymerase chain reaction and immunohistochemical analysis were used to detect the expression levels of HJURP in benign and PCa prostate tissues. The Taylor dataset was statistically analyzed to determine if HJURP expression levels were associated with PCa clinicopathological data. HJURP was overexpressed in PCa tissues compared with benign prostate tissues. Statistical analysis of the Taylor dataset indicated that upregulation of HJURP was significantly associated with positive prostate‑specific antigen (PSA) levels (P=0.004), high Gleason score (P=0.005), advanced pathological stage (P=0.007), metastasis (P<0.001) and PSA failure (P<0.001). Higher HJURP mRNA expression levels were significantly associated with shorter biochemical recurrence (BCR)‑free survival (P<0.001). To the best of our knowledge, this study is the first report of HJURP upregulation in PCa tissues. Upregulation of HJURP may predict BCR‑free survival and HJURP may be an oncogene that impacts the prognosis of patients with PCa.

View Figures

View References

1	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2018. CA Cancer J Clin. 68:7–30. 2018. View Article : Google Scholar : PubMed/NCBI
2	Artibani W, Porcaro AB, De Marco V, Cerruto MA and Siracusano S: Management of biochemical recurrence after primary curative treatment for prostate cancer: A review. Urol Int. 100:251–262. 2018. View Article : Google Scholar : PubMed/NCBI
3	Spahn M, Boxler S, Joniau S, Moschini M, Tombal B and Karnes RJ: What is the need for prostatic biomarkers in prostate cancer management? Curr Urol Rep. 16:702015. View Article : Google Scholar : PubMed/NCBI
4	Gaither TL, Merrett SL, Pun MJ and Scott KC: Centromeric barrier disruption leads to mitotic defects in schizosaccharomyces pombe. G3 (Bethesda). 4:633–642. 2014. View Article : Google Scholar : PubMed/NCBI
5	Barnhart MC, Kuich PH, Stellfox ME, Ward JA, Bassett EA, Black BE and Foltz DR: HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore. J Cell Biol. 194:229–243. 2011. View Article : Google Scholar : PubMed/NCBI
6	Mishra PK, Au WC, Choy JS, Kuich PH, Baker RE, Foltz DR and Basrai MA: Misregulation of Scm3p/HJURP causes chromosome instability in saccharomyces cerevisiae and human cells. PLoS Genet. 7:e10023032011. View Article : Google Scholar : PubMed/NCBI
7	Zhang W, Mao JH, Zhu W, Jain AK, Liu K, Brown JB and Karpen GH: Centromere and kinetochore gene misexpression predicts cancer patient survival and response to radiotherapy and chemotherapy. Nat Commun. 7:126192016. View Article : Google Scholar : PubMed/NCBI
8	Verdaasdonk JS and Bloom K: Centromeres: Unique chromatin structures that drive chromosome segregation. Nat Rev Mol Cell Biol. 12:320–332. 2011. View Article : Google Scholar : PubMed/NCBI
9	Foltz DR, Jansen LE, Bailey AO, Yates JR III, Bassett EA, Wood S, Black BE and Cleveland DW: Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP. Cell. 137:472–484. 2009. View Article : Google Scholar : PubMed/NCBI
10	Valente V, Serafim RB, de Oliveira LC, Adorni FS, Torrieri R, Tirapelli DP, Espreafico EM, Oba-Shinjo SM, Marie SK, Paçó-Larson ML and Carlotti CG Jr: Modulation of HJURP (Holliday Junction-Recognizing Protein) levels is correlated with glioblastoma cells survival. PLoS One. 8:e622002013. View Article : Google Scholar : PubMed/NCBI
11	Hu Z, Huang G, Sadanandam A, Gu S, Lenburg ME, Pai M, Bayani N, Blakely EA, Gray JW and Mao JH: The expression level of HJURP has an independent prognostic impact and predicts the sensitivity to radiotherapy in breast cancer. Breast Cancer Res. 12:R182010. View Article : Google Scholar : PubMed/NCBI
12	Rouam S, Moreau T and Broët P: Identifying common prognostic factors in genomic cancer studies: A novel index for censored outcomes. BMC Bioinformatics. 11:1502010. View Article : Google Scholar : PubMed/NCBI
13	Kato T, Sato N, Hayama S, Yamabuki T, Ito T, Miyamoto M, Kondo S, Nakamura Y and Daigo Y: Activation of holliday junction recognizing protein involved in the chromosomal stability and immortality of cancer cells. Cancer Res. 67:8544–8553. 2007. View Article : Google Scholar : PubMed/NCBI
14	Taylor BS, Schultz N, Hieronymus H, Gopalan A, Xiao Y, Carver BS, Arora VK, Kaushik P, Cerami E, Reva B, et al: Integrative genomic profiling of human prostate cancer. Cancer Cell. 18:11–22. 2010. View Article : Google Scholar : PubMed/NCBI
15	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
16	Brimo F, Montironi R, Egevad L, Erbersdobler A, Lin DW, Nelson JB, Rubin MA, van der Kwast T, Amin M and Epstein JI: Contemporary grading for prostate cancer: Implications for patient care. Eur Urol. 63:892–901. 2013. View Article : Google Scholar : PubMed/NCBI
17	Kweldam CF, van Leenders GJ and van der Kwast T: Grading of prostate cancer: A work in progress. Histopathology. 74:146–160. 2019. View Article : Google Scholar : PubMed/NCBI
18	Soares R and Eden CG: Surgical treatment of high-risk prostate cancer. Minerva Urol Nefrol. 67:33–46. 2015.PubMed/NCBI
19	Giacalone NJ, Wu J, Chen MH, Renshaw A, Loffredo M, Kantoff PW and D'Amico AV: Prostate-specific antigen failure and risk of death within comorbidity subgroups among men with unfavorable-risk prostate cancer treated in a randomized trial. J Clin Oncol. 34:3781–3786. 2016. View Article : Google Scholar : PubMed/NCBI
20	Liao WT, Feng Y, Li ML, Liu GL, Li MZ, Zeng MS and Song LB: Overexpression of centromere protein H is significantly associated with breast cancer progression and overall patient survival. Chin J Cancer. 30:627–637. 2011. View Article : Google Scholar : PubMed/NCBI
21	McGovern SL, Qi Y, Pusztai L, Symmans WF and Buchholz TA: Centromere protein-A, an essential centromere protein, is a prognostic marker for relapse in estrogen receptor-positive breast cancer. Breast Cancer Res. 14:R722012. View Article : Google Scholar : PubMed/NCBI
22	Dai Y, Liu L, Zeng T, Zhu YH, Li J, Chen L, Li Y, Yuan YF, Ma S and Guan XY: Characterization of the oncogenic function of centromere protein F in hepatocellular carcinoma. Biochem Biophys Res Commun. 436:711–718. 2013. View Article : Google Scholar : PubMed/NCBI
23	Georgoulis A, Vorgias CE, Chrousos GP and Rogakou EP: Genome instability and γH2AX. Int J Mol Sci. 18:E19792017. View Article : Google Scholar : PubMed/NCBI
24	Nagaria P, Robert C and Rassool FV: DNA double-strand break response in stem cells: Mechanisms to maintain genomic integrity. Biochim Biophys Acta. 1830:2345–2353. 2013. View Article : Google Scholar : PubMed/NCBI
25	Majidinia M and Yousefi B: DNA damage response regulation by microRNAs as a therapeutic target in cancer. DNA Repair (Amst). 47:1–11. 2016. View Article : Google Scholar : PubMed/NCBI
26	Raschellà G, Melino G and Malewicz M: New factors in mammalian DNA repair-the chromatin connection. Oncogene. 36:4673–4681. 2017. View Article : Google Scholar : PubMed/NCBI
27	Zhen Y, Xiao R, Chen X, Yuan C, Sun Y and Li J: A non-synonymous polymorphism is associated with progression from chronic hepatitis B virus infection to hepatocellular carcinoma in a Chinese population. Onco Targets Ther. 11:563–569. 2018. View Article : Google Scholar : PubMed/NCBI