Open Access

Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer

  • Authors:
    • Zhan Wang
    • Zhen Xiang
    • Ting Zhu
    • Juan Chen
    • Mei‑Zuo Zhong
    • Juan Huang
    • Kuan‑Song Wang
    • Ling Li
    • Lun‑Quan Sun
    • Wei‑Bing Zhou
  • View Affiliations

  • Published online on: November 7, 2019     https://doi.org/10.3892/ol.2019.11067
  • Pages: 167-176
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cathepsin L (CTSL) is a lysosomal acid cysteine protease that has been implicated in tumorigenesis and malignant progression. In the present study, the role of CTSL in tumorigenesis and prognosis of breast cancer was evaluated. The prognostic value of CTSL was analyzed using immunohistochemistry in patients with breast cancer, as well as online microarray datasets. CTSL expression was knocked down in the breast cancer cell line T‑47D using RNA interference. MTT and colony formation assays were performed to assess the role of CTSL in the proliferation of breast cancer cells. Cell cycle progression and apoptosis were measured using flow cytometry. A physical interaction of CTSL and cyclin dependent kinase 2 associated protein 1 (CDK2‑AP1) was determined using a glutathione S‑transferase pull‑down assay. Endogenous CTSL expression was high in breast cancer cells and exhibited an inverse association with CDK2‑AP1 expression; aberrant expression of CTSL in breast cancer tissues predicted an improved clinical outcome and prognosis. In addition, CTSL knockdown decelerated the progression of breast cancer cells by arresting cell cycle progression and increasing apoptosis. Thus, CTSL may be a potential therapeutic target for treating patients with breast cancer.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wang Z, Xiang Z, Zhu T, Chen J, Zhong MZ, Huang J, Wang KS, Li L, Sun LQ, Zhou WB, Zhou WB, et al: Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer. Oncol Lett 19: 167-176, 2020
APA
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J. ... Zhou, W. (2020). Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer. Oncology Letters, 19, 167-176. https://doi.org/10.3892/ol.2019.11067
MLA
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J., Wang, K., Li, L., Sun, L., Zhou, W."Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer". Oncology Letters 19.1 (2020): 167-176.
Chicago
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J., Wang, K., Li, L., Sun, L., Zhou, W."Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer". Oncology Letters 19, no. 1 (2020): 167-176. https://doi.org/10.3892/ol.2019.11067