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Article Open Access

Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer

  • Authors:
    • Zhan Wang
    • Zhen Xiang
    • Ting Zhu
    • Juan Chen
    • Mei‑Zuo Zhong
    • Juan Huang
    • Kuan‑Song Wang
    • Ling Li
    • Lun‑Quan Sun
    • Wei‑Bing Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Hunan Province Clinic Meditech Research Center for Breast Cancer, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China, Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 167-176
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    Published online on: November 7, 2019
       https://doi.org/10.3892/ol.2019.11067
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Abstract

Cathepsin L (CTSL) is a lysosomal acid cysteine protease that has been implicated in tumorigenesis and malignant progression. In the present study, the role of CTSL in tumorigenesis and prognosis of breast cancer was evaluated. The prognostic value of CTSL was analyzed using immunohistochemistry in patients with breast cancer, as well as online microarray datasets. CTSL expression was knocked down in the breast cancer cell line T‑47D using RNA interference. MTT and colony formation assays were performed to assess the role of CTSL in the proliferation of breast cancer cells. Cell cycle progression and apoptosis were measured using flow cytometry. A physical interaction of CTSL and cyclin dependent kinase 2 associated protein 1 (CDK2‑AP1) was determined using a glutathione S‑transferase pull‑down assay. Endogenous CTSL expression was high in breast cancer cells and exhibited an inverse association with CDK2‑AP1 expression; aberrant expression of CTSL in breast cancer tissues predicted an improved clinical outcome and prognosis. In addition, CTSL knockdown decelerated the progression of breast cancer cells by arresting cell cycle progression and increasing apoptosis. Thus, CTSL may be a potential therapeutic target for treating patients with breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Z, Xiang Z, Zhu T, Chen J, Zhong MZ, Huang J, Wang KS, Li L, Sun LQ, Zhou WB, Zhou WB, et al: Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer. Oncol Lett 19: 167-176, 2020.
APA
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J. ... Zhou, W. (2020). Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer. Oncology Letters, 19, 167-176. https://doi.org/10.3892/ol.2019.11067
MLA
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J., Wang, K., Li, L., Sun, L., Zhou, W."Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer". Oncology Letters 19.1 (2020): 167-176.
Chicago
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J., Wang, K., Li, L., Sun, L., Zhou, W."Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer". Oncology Letters 19, no. 1 (2020): 167-176. https://doi.org/10.3892/ol.2019.11067
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Z, Xiang Z, Zhu T, Chen J, Zhong MZ, Huang J, Wang KS, Li L, Sun LQ, Zhou WB, Zhou WB, et al: Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer. Oncol Lett 19: 167-176, 2020.
APA
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J. ... Zhou, W. (2020). Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer. Oncology Letters, 19, 167-176. https://doi.org/10.3892/ol.2019.11067
MLA
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J., Wang, K., Li, L., Sun, L., Zhou, W."Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer". Oncology Letters 19.1 (2020): 167-176.
Chicago
Wang, Z., Xiang, Z., Zhu, T., Chen, J., Zhong, M., Huang, J., Wang, K., Li, L., Sun, L., Zhou, W."Cathepsin L interacts with CDK2‑AP1 as a potential predictor of prognosis in patients with breast cancer". Oncology Letters 19, no. 1 (2020): 167-176. https://doi.org/10.3892/ol.2019.11067
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