Silencing of FoxM1 blocks growth, migration and invasion of papillary thyroid carcinoma cells

Wang,Xiaotao; Zhao,Yongkui; Cui,Mingxin; Chen,Jianli; Cao,Wenbin; Yang,Guanghua; Liu,Qianyu; Zhang,Guozhi

doi:10.3892/ol.2019.11108

Silencing of FoxM1 blocks growth, migration and invasion of papillary thyroid carcinoma cells

Authors:
- Xiaotao Wang
- Yongkui Zhao
- Mingxin Cui
- Jianli Chen
- Wenbin Cao
- Guanghua Yang
- Qianyu Liu
- Guozhi Zhang
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Affiliations: Department of General Surgery, Affiliated Hospital of North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China
Published online on: November 18, 2019 https://doi.org/10.3892/ol.2019.11108
Pages: 77-82
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of Forkhead box transcription factor M1 (FoxM1)‑silencing on the growth, migration and invasion of K1 human papillary thyroid carcinoma (PTC) cells. The effect of FoxM1‑small interfering RNA (siRNA) in K1 cells was detected by western blot analysis. FoxM1‑siRNA and control siRNA were transfected into K1 cells using Lipofectamine® 2000 (transfection group, T) and the non‑meaning sequence group (NM). K1 cells exposed to PBS solution comprised the blank control group (CON). Cell proliferation ability was detected using an MTT assay. Cell migration and invasion was detected by the single cell scratch test and Transwell invasion assay, respectively. Western blot analysis indicated that FoxM1 siRNA downregulated the expression of FoxM1 protein. Cell proliferation, migration and invasion were significantly lower in the T group compared with the NM and CON groups (P<0.05). These results indicated that silencing of FoxM1 expression could block growth, invasion and migration of K1 cells. This study may provide a novel target gene for targeted therapy of PTC.

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