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Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome

  • Authors:
    • Xinyan Jia
    • Wenzhong Yang
    • Xiaohui Zhou
    • Lu Han
    • Jumei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China, Department of Hematology, Shanghai Tenth People's Hospital, Tongji University Cancer Center, Tongji University School of Medicine, Shanghai 200072, P.R. China
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 442-448
    |
    Published online on: November 19, 2019
       https://doi.org/10.3892/ol.2019.11114
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Abstract

Myelodysplastic syndrome (MDS) represents a heterogeneous hematopoietic disorder in which mature blood cells are derived from an abnormal multipotent progenitor cell. The current therapy for MDS involves repeated cycles of DNA methyltransferase (DNMT) inhibitors, particularly the demethylation drug 5‑azacytidine (5‑azaC) which has been shown to increase the survival of patients with high‑risk MDS. The mechanisms behind the therapeutic effects of 5‑azaC are not yet clear. In this study the effect of 5‑azaC on the development of regulatory T cells (Tregs) and T‑helper 17 (Th17) cells was investigated. The numbers of CD4+ T‑cell subsets in 30 patients with intermediate‑2/high‑risk MDS were serially assessed at diagnosis and following 5‑azaC treatment. The number of FoxP3+ Tregs was significantly higher after 3 months of therapy. However, there was no statistical difference in the number of Th17 cells following treatment. In vitro, 5‑azaC enhanced the overall proportion of Tregs, but not Th17, in CD4+ T cells from patients with MDS. Addition of 5‑azaC reduced the proliferative capacity of Tregs, suggesting that the increase in Tregs was due to conversion of conventional CD25‑ cells, rather than proliferation of CD25+FoxP3+ cells. The FoxP3 expression in 5‑azaC‑treated T effectors was also increased. Interestingly, while Tbet and RORγT mRNA transcription had no obvious changes, due to the demethylation of the FoxP3 promoter, these findings are important in associating the induction of DNA hypomethylation and the clinical response to 5‑azaC.
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Copy and paste a formatted citation
Spandidos Publications style
Jia X, Yang W, Zhou X, Han L and Shi J: Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome. Oncol Lett 19: 442-448, 2020.
APA
Jia, X., Yang, W., Zhou, X., Han, L., & Shi, J. (2020). Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome. Oncology Letters, 19, 442-448. https://doi.org/10.3892/ol.2019.11114
MLA
Jia, X., Yang, W., Zhou, X., Han, L., Shi, J."Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome". Oncology Letters 19.1 (2020): 442-448.
Chicago
Jia, X., Yang, W., Zhou, X., Han, L., Shi, J."Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome". Oncology Letters 19, no. 1 (2020): 442-448. https://doi.org/10.3892/ol.2019.11114
Copy and paste a formatted citation
x
Spandidos Publications style
Jia X, Yang W, Zhou X, Han L and Shi J: Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome. Oncol Lett 19: 442-448, 2020.
APA
Jia, X., Yang, W., Zhou, X., Han, L., & Shi, J. (2020). Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome. Oncology Letters, 19, 442-448. https://doi.org/10.3892/ol.2019.11114
MLA
Jia, X., Yang, W., Zhou, X., Han, L., Shi, J."Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome". Oncology Letters 19.1 (2020): 442-448.
Chicago
Jia, X., Yang, W., Zhou, X., Han, L., Shi, J."Influence of demethylation on regulatory T and Th17 cells in myelodysplastic syndrome". Oncology Letters 19, no. 1 (2020): 442-448. https://doi.org/10.3892/ol.2019.11114
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