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Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used

  • Authors:
    • Lyubomir Haralambiev
    • Lasse Wien
    • Nadine Gelbrich
    • Jörn Lange
    • Sinan Bakir
    • Axel Kramer
    • Martin Burchardt
    • Axel Ekkernkamp
    • Denis Gümbel
    • Matthias B. Stope
  • View Affiliations / Copyright

    Affiliations: Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, D‑17475 Greifswald, Germany, Department of Urology, University Medicine Greifswald, D‑17475 Greifswald, Germany, Institute of Hygiene and Environmental Medicine, University Medicine Greifswald, D‑17475 Greifswald, Germany
    Copyright: © Haralambiev et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 283-290
    |
    Published online on: November 19, 2019
       https://doi.org/10.3892/ol.2019.11115
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Abstract

Osteosarcoma (OS) is the most common tumor of the musculoskeletal system. Recently, cold atmospheric plasma (CAP) has been regarded as a promising anti‑oncogenic therapy. Previous experimental studies have demonstrated that CAP treatment results in significant growth inhibition of human sarcoma and is able to induce apoptosis. However, due to device‑specific parameters, there is a large variability in the antitumor effects of different CAP sources. In the present study, the cellular effects of CAP treatment from two different CAP devices were investigated and their pro‑apoptotic efficacy was characterized. The OS cell lines, U2‑OS and MNNG/HOS, were treated with two CAP devices, kINPen MED and MiniJet‑R. Control groups were treated with argon. The anti‑proliferative effect of each treatment was demonstrated using cell counting and the activation of apoptotic mechanisms was determined using Comet, TUNEL and Caspase‑3/Caspase‑7 assays. The results revealed that treatment of both OS cell lines with the two CAP sources resulted in significant inhibition of cell growth. Subsequently, the activation of Caspases and the induction of apoptotic DNA fragmentation was demonstrated. The biological effects of each CAP source did not differ significantly. The treatment of OS cells with CAP lead to an induction of apoptosis and a reduction of cell growth. Therefore, the biological effects of CAP appear to be general as the two devices of different design produced highly comparable cell responses. Therefore, the type of device used does not seem to affect the efficacy of CAP‑based antitumor therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Haralambiev L, Wien L, Gelbrich N, Lange J, Bakir S, Kramer A, Burchardt M, Ekkernkamp A, Gümbel D, Stope MB, Stope MB, et al: Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used. Oncol Lett 19: 283-290, 2020.
APA
Haralambiev, L., Wien, L., Gelbrich, N., Lange, J., Bakir, S., Kramer, A. ... Stope, M.B. (2020). Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used. Oncology Letters, 19, 283-290. https://doi.org/10.3892/ol.2019.11115
MLA
Haralambiev, L., Wien, L., Gelbrich, N., Lange, J., Bakir, S., Kramer, A., Burchardt, M., Ekkernkamp, A., Gümbel, D., Stope, M. B."Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used". Oncology Letters 19.1 (2020): 283-290.
Chicago
Haralambiev, L., Wien, L., Gelbrich, N., Lange, J., Bakir, S., Kramer, A., Burchardt, M., Ekkernkamp, A., Gümbel, D., Stope, M. B."Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used". Oncology Letters 19, no. 1 (2020): 283-290. https://doi.org/10.3892/ol.2019.11115
Copy and paste a formatted citation
x
Spandidos Publications style
Haralambiev L, Wien L, Gelbrich N, Lange J, Bakir S, Kramer A, Burchardt M, Ekkernkamp A, Gümbel D, Stope MB, Stope MB, et al: Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used. Oncol Lett 19: 283-290, 2020.
APA
Haralambiev, L., Wien, L., Gelbrich, N., Lange, J., Bakir, S., Kramer, A. ... Stope, M.B. (2020). Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used. Oncology Letters, 19, 283-290. https://doi.org/10.3892/ol.2019.11115
MLA
Haralambiev, L., Wien, L., Gelbrich, N., Lange, J., Bakir, S., Kramer, A., Burchardt, M., Ekkernkamp, A., Gümbel, D., Stope, M. B."Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used". Oncology Letters 19.1 (2020): 283-290.
Chicago
Haralambiev, L., Wien, L., Gelbrich, N., Lange, J., Bakir, S., Kramer, A., Burchardt, M., Ekkernkamp, A., Gümbel, D., Stope, M. B."Cold atmospheric plasma inhibits the growth of osteosarcoma cells by inducing apoptosis, independent of the device used". Oncology Letters 19, no. 1 (2020): 283-290. https://doi.org/10.3892/ol.2019.11115
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