Open Access

Identification and chemoresistance of cancer stem cells in HPV‑negative oropharyngeal cancer

  • Authors:
    • Mehmet Gunduz
    • Esra Gunduz
    • Shunji Tamagawa
    • Keisuke Enomoto
    • Muneki Hotomi
  • View Affiliations

  • Published online on: November 21, 2019     https://doi.org/10.3892/ol.2019.11127
  • Pages: 965-971
  • Copyright: © Gunduz et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The underlying mechanisms of resistance to chemoradiotherapy of human papilloma virus (HPV)‑negative patients with oropharyngeal cancer (OPC) remain unclear. The present study aimed to characterize cancer stem cells (CSC) of the HPV‑negative OPC cell line in terms of chemotherapy resistance. CSCs were isolated through magnetic activated cell sorting using the CSC specific marker aldehyde dehydrogenase 1 antibody, and characterized by sphere formation capacity, immunofluorescence staining, and CSC marker expression. CSC response to cisplatin treatment was evaluated via XTT‑assays. Spheres of CSCs of the HPV‑negative UTSCC‑60A cell line were highly dark holospheres. RNA expression levels of CSC markers OCT4, SOX2, Kruppel‑like factor 4 and BMI1 were significantly higher in CSC. CSCs were significantly resistant to cisplatin treatment at various dosages compared with nonCSC. The present study suggested that the proportion of CSCs is very low in the tumor bulk, CSCs are resistant to cisplatin in HPV‑negative OPC, which requires further investigation to define their mechanism.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Gunduz M, Gunduz E, Tamagawa S, Enomoto K and Hotomi M: Identification and chemoresistance of cancer stem cells in HPV‑negative oropharyngeal cancer. Oncol Lett 19: 965-971, 2020.
APA
Gunduz, M., Gunduz, E., Tamagawa, S., Enomoto, K., & Hotomi, M. (2020). Identification and chemoresistance of cancer stem cells in HPV‑negative oropharyngeal cancer. Oncology Letters, 19, 965-971. https://doi.org/10.3892/ol.2019.11127
MLA
Gunduz, M., Gunduz, E., Tamagawa, S., Enomoto, K., Hotomi, M."Identification and chemoresistance of cancer stem cells in HPV‑negative oropharyngeal cancer". Oncology Letters 19.1 (2020): 965-971.
Chicago
Gunduz, M., Gunduz, E., Tamagawa, S., Enomoto, K., Hotomi, M."Identification and chemoresistance of cancer stem cells in HPV‑negative oropharyngeal cancer". Oncology Letters 19, no. 1 (2020): 965-971. https://doi.org/10.3892/ol.2019.11127