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Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line

  • Authors:
    • Yaqin Zhang
    • Xiaotong Zhao
    • Mingwei Chen
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 892-898
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    Published online on: November 21, 2019
       https://doi.org/10.3892/ol.2019.11131
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Abstract

Omentin‑1, a 34‑kDa protein, has been demonstrated to be associated with colorectal cancer (CRC). Epidemiological and clinical studies have indicated that the levels of circulating omentin‑1 are significantly increased in patients with CRC, but the cause of the high omentin‑1 levels and whether CRC cells express this adipokine have not been determined. In the present study, human colorectal carcinoma and para‑carcinoma tissues were collected from 24 patients with CRC. In addition, SW480 and HCT116 colon cancer cells were cultured in vitro. The expression and localization of omentin‑1 protein in human CRC and para‑carcinoma tissues were determined by immunohistochemistry. The mRNA and protein expression levels of omentin‑1 in human CRC and para‑carcinoma tissues were detected by reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting, respectively. In addition, omentin‑1 mRNA expression levels in SW480 and HCT116 cell lines were detected by RT‑qPCR. Since SW480 cells exhibited higher omentin‑1 mRNA levels compared with those of HCT116 cells, SW480 cells were selected for further experiments. The expression of omentin‑1 protein in the supernatant and lysate of SW480 cells obtained at 6, 12, 24 and 48 h was determined by ELISA. The immunohistochemistry results demonstrated that the positive expression of omentin‑1 protein was mainly located in the cytoplasm of cancer cells in human CRC tissues. The mRNA and protein expression levels of omentin‑1 in the CRC tissues were higher compared with those in para‑carcinoma tissues. The expression levels of omentin‑1 were detected in the cell lysate and supernatant of SW480 cells; the expression level of omentin‑1 protein in the cell lysate was higher compared with that in the supernatant. These results indicated that SW480 cells secret and express the adipokine omentin‑1 endogenously. Omentin‑1 may serve its potential carcinogenetic role in CRC through endocrine, autocrine and paracrine pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Zhao X and Chen M: Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line. Oncol Lett 19: 892-898, 2020.
APA
Zhang, Y., Zhao, X., & Chen, M. (2020). Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line. Oncology Letters, 19, 892-898. https://doi.org/10.3892/ol.2019.11131
MLA
Zhang, Y., Zhao, X., Chen, M."Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line". Oncology Letters 19.1 (2020): 892-898.
Chicago
Zhang, Y., Zhao, X., Chen, M."Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line". Oncology Letters 19, no. 1 (2020): 892-898. https://doi.org/10.3892/ol.2019.11131
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Zhao X and Chen M: Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line. Oncol Lett 19: 892-898, 2020.
APA
Zhang, Y., Zhao, X., & Chen, M. (2020). Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line. Oncology Letters, 19, 892-898. https://doi.org/10.3892/ol.2019.11131
MLA
Zhang, Y., Zhao, X., Chen, M."Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line". Oncology Letters 19.1 (2020): 892-898.
Chicago
Zhang, Y., Zhao, X., Chen, M."Autocrine action of adipokine omentin‑1 in the SW480 colon cancer cell line". Oncology Letters 19, no. 1 (2020): 892-898. https://doi.org/10.3892/ol.2019.11131
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