Open Access

miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A

Retraction in: /10.3892/ol.2020.12182

  • Authors:
    • Xiaohan Sun
    • Wenrui Xu
    • Chuanshan Zang
    • Na Li
  • View Affiliations

  • Published online on: November 25, 2019     https://doi.org/10.3892/ol.2019.11144
  • Pages: 771-776
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Biological function of microRNA‑20c‑3p (miRNA‑520c‑3p) in the progression of nasopharyngeal carcinoma (NPC) and the potential mechanism were investigated. Relative level of miRNA‑520c‑3p in NPC tissues and adjacent normal tissues was determined by quantitative real‑time polymerase chain reaction (qRT‑PCR). Particularly, miRNA‑520c‑3p level in NPC with different tumor stages and tumor sizes was examined. Subsequently, miRNA‑520c‑3p level in nasopharyngeal epithelial cells and NPC cells was detected. The potential influence of miRNA‑520c‑3p on the proliferative ability and cell cycle progression of NPC cells were evaluated through cell counting kit‑8 (CCK‑8) and flow cytometry. The target gene of miRNA‑520c‑3p was verified by dual‑luciferase reporter gene assay. Regulatory role of miRNA‑520c‑3p/RAB22A in the malignant progression of NPC was identified. miRNA‑520c‑3p was downregulated in NPC tissues and cell lines. Its level was lower in NPC with worse tumor grade and larger tumor size. Overexpression of miRNA‑520c‑3p suppressed the proliferative ability and arrested cell cycle in G0/G1 phase. RAB22A was confirmed to be the downstream target of miRNA‑520c‑3p. In NPC tissues and cell lines, RAB22A remained in higher abundance relative to controls. Overexpression of RAB22A reversed the inhibitory effects of overexpressed miRNA‑520c‑3p on proliferative ability and cell cycle progression of NPC cells. miRNA‑520c‑3p is downregulated in NPC, which accelerates the malignant progression of NPC by targeting RAB22A.
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January-2020
Volume 19 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Sun X, Xu W, Zang C and Li N: miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A Retraction in /10.3892/ol.2020.12182. Oncol Lett 19: 771-776, 2020
APA
Sun, X., Xu, W., Zang, C., & Li, N. (2020). miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A Retraction in /10.3892/ol.2020.12182. Oncology Letters, 19, 771-776. https://doi.org/10.3892/ol.2019.11144
MLA
Sun, X., Xu, W., Zang, C., Li, N."miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A Retraction in /10.3892/ol.2020.12182". Oncology Letters 19.1 (2020): 771-776.
Chicago
Sun, X., Xu, W., Zang, C., Li, N."miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A Retraction in /10.3892/ol.2020.12182". Oncology Letters 19, no. 1 (2020): 771-776. https://doi.org/10.3892/ol.2019.11144