miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A Retraction in /10.3892/ol.2020.12182

Sun,Xiaohan; Xu,Wenrui; Zang,Chuanshan; Li,Na

doi:10.3892/ol.2019.11144

January-2020 Volume 19 Issue 1

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January-2020 Volume 19 Issue 1

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Article Open Access

miRNA‑520c‑3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A

Retraction in: /10.3892/ol.2020.12182

Authors:
- Xiaohan Sun
- Wenrui Xu
- Chuanshan Zang
- Na Li
View Affiliations / Copyright

Affiliations: Department of Otolaryngology Head and Neck Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China

Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 771-776
|
Published online on: November 25, 2019

https://doi.org/10.3892/ol.2019.11144
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Abstract

Biological function of microRNA‑20c‑3p (miRNA‑520c‑3p) in the progression of nasopharyngeal carcinoma (NPC) and the potential mechanism were investigated. Relative level of miRNA‑520c‑3p in NPC tissues and adjacent normal tissues was determined by quantitative real‑time polymerase chain reaction (qRT‑PCR). Particularly, miRNA‑520c‑3p level in NPC with different tumor stages and tumor sizes was examined. Subsequently, miRNA‑520c‑3p level in nasopharyngeal epithelial cells and NPC cells was detected. The potential influence of miRNA‑520c‑3p on the proliferative ability and cell cycle progression of NPC cells were evaluated through cell counting kit‑8 (CCK‑8) and flow cytometry. The target gene of miRNA‑520c‑3p was verified by dual‑luciferase reporter gene assay. Regulatory role of miRNA‑520c‑3p/RAB22A in the malignant progression of NPC was identified. miRNA‑520c‑3p was downregulated in NPC tissues and cell lines. Its level was lower in NPC with worse tumor grade and larger tumor size. Overexpression of miRNA‑520c‑3p suppressed the proliferative ability and arrested cell cycle in G0/G1 phase. RAB22A was confirmed to be the downstream target of miRNA‑520c‑3p. In NPC tissues and cell lines, RAB22A remained in higher abundance relative to controls. Overexpression of RAB22A reversed the inhibitory effects of overexpressed miRNA‑520c‑3p on proliferative ability and cell cycle progression of NPC cells. miRNA‑520c‑3p is downregulated in NPC, which accelerates the malignant progression of NPC by targeting RAB22A.

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