Knockdown of ST7-AS1 inhibits migration, invasion, cell cycle progression and induces apoptosis of gastric cancer

Cai,Shuang; Weng,Yang; Liu,Pengliang; Miao,Feng

doi:10.3892/ol.2019.11145

Knockdown of ST7-AS1 inhibits migration, invasion, cell cycle progression and induces apoptosis of gastric cancer

Authors:
- Shuang Cai
- Yang Weng
- Pengliang Liu
- Feng Miao
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Affiliations: The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China
Published online on: November 25, 2019 https://doi.org/10.3892/ol.2019.11145
Pages: 777-782
Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Role of ST7‑AS1 in the malignant progression of gastric cancer (GC) and its molecular mechanisms were investigated. ST7‑AS1 level in GC tissues and matched normal tissues was determined by quantitative real‑time polymerase chain reaction (qRT‑PCR). Its level in GC patients presenting different tumor stages and tumor sizes was determined. Subsequently, ST7‑AS1 level in epithelial cells of gastric mucosa and GC cell lines was examined. Cellular behavior of GC cells, including viability, apoptosis, migration, invasion and cell cycle, influenced by ST7‑AS1 was evaluated. The interaction between ST7‑AS1 and EZH2 was assessed by RNA immunoprecipitation (RIP) assay. The involvement of EZH2 in the progression of GC mediated by ST7‑AS1 was identified. ST7‑AS1 was upregulated in GC tissues and cell lines. Its level was positively correlated to tumor stage and tumor size of GC. Knockdown of ST7‑AS1 attenuated proliferative, migratory and invasive abilities, arrested cell cycle progression and induced apoptosis of GC cells. EZH2 was identified to interact with ST7‑AS1, which attenuated the regulatory effects of ST7‑AS1 on migratory and invasive abilities of GC cells. Upregulated ST7‑AS1 in GC accelerated proliferation, migration and invasion, and inhibited apoptosis, thus aggravating the progression of GC.

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