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Article Open Access

NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways

  • Authors:
    • Fei Ren
    • Wei Zhang
    • Shuai Lu
    • Hong Ren
    • Yantong Guo
  • View Affiliations / Copyright

    Affiliations: Department of Medicine, Peking University, Beijing 100191, P.R. China, Department of Breast Surgery, Affiliated Hospital, Shanxi Traditional Chinese Medical University, Taiyuan, Shanxi 030024, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 813-823
    |
    Published online on: November 28, 2019
       https://doi.org/10.3892/ol.2019.11152
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Abstract

Breast cancer is a leading cause of cancer‑associated mortality globally amongst gynecologic tumors due to aggressive metastasis. A previous study reported that neurensin‑2 (NRSN2) was implicated in human cancer cells, and that NRSN2 gene and protein expression levels were significantly upregulated in human breast cancer tissues compared with adjacent non‑tumor tissues. The purpose of the present study was to analyze the role of NRSN2 in the metastasis of breast cancer cells and explore its potential mechanism. Reverse transcription‑quantitative PCR, MTT, western blotting and immunohistochemistry was used to analyze the role of NRSN2 both in vitro and in vivo. The present study demonstrated that NRSN2 knockdown inhibited the proliferation, migration and invasion of breast cancer cells in vitro. NRSN2 upregulation promoted breast cancer cell proliferation and tissue growth in vitro and in vivo. In addition, the results demonstrated that the regulatory effects of NRSN2 on breast cancer cells were associated with PI3K/AKT/mTOR and NF‑κB signaling pathway dysregulation. Furthermore, NRSN2 overexpression in mice significantly promoted breast cancer cell proliferation. In conclusion, the results from the present study indicated that NRSN2 may be considered as a novel oncogenic protein and may represent a potential therapeutic target for breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Ren F, Zhang W, Lu S, Ren H and Guo Y: NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways. Oncol Lett 19: 813-823, 2020.
APA
Ren, F., Zhang, W., Lu, S., Ren, H., & Guo, Y. (2020). NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways. Oncology Letters, 19, 813-823. https://doi.org/10.3892/ol.2019.11152
MLA
Ren, F., Zhang, W., Lu, S., Ren, H., Guo, Y."NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways". Oncology Letters 19.1 (2020): 813-823.
Chicago
Ren, F., Zhang, W., Lu, S., Ren, H., Guo, Y."NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways". Oncology Letters 19, no. 1 (2020): 813-823. https://doi.org/10.3892/ol.2019.11152
Copy and paste a formatted citation
x
Spandidos Publications style
Ren F, Zhang W, Lu S, Ren H and Guo Y: NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways. Oncol Lett 19: 813-823, 2020.
APA
Ren, F., Zhang, W., Lu, S., Ren, H., & Guo, Y. (2020). NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways. Oncology Letters, 19, 813-823. https://doi.org/10.3892/ol.2019.11152
MLA
Ren, F., Zhang, W., Lu, S., Ren, H., Guo, Y."NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways". Oncology Letters 19.1 (2020): 813-823.
Chicago
Ren, F., Zhang, W., Lu, S., Ren, H., Guo, Y."NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF‑κB signaling pathways". Oncology Letters 19, no. 1 (2020): 813-823. https://doi.org/10.3892/ol.2019.11152
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