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Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer

  • Authors:
    • Taxiarchis Konstantinos Nikolouzakis
    • Polychronis D. Stivaktakis
    • Paraskevi Apalaki
    • Katerina Kalliantasi
    • Theodoros Mariolis Sapsakos
    • Demetrios A. Spandidos
    • Aristidis Tsatsakis
    • John Souglakos
    • John Tsiaoussis
  • View Affiliations / Copyright

    Affiliations: Laboratory of Anatomy-Histology-Embryology, Medical School, University of Crete, 71110 Heraklion, Greece, Laboratory of Toxicology, Medical School, University of Crete, 71409 Heraklion, Greece, Department of Forensic Sciences and Toxicology, Medical School, University of Crete, 71003 Heraklion, Greece, Laboratory of Anatomy and Histology, Nursing School, National and Kapodistrian University of Athens, 11527 Athens, Greece, Laboratory of Clinical Virology, Medical School, University of Crete, 71003 Heraklion, Greece, Department of Medical Oncology, University General Hospital of Heraklion, 71110 Heraklion, Greece
    Copyright: © Nikolouzakis et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2703-2712
    |
    Published online on: January 7, 2019
       https://doi.org/10.3892/ol.2019.9895
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Abstract

Colorectal cancer (CRC) is the third most diagnosed type of cancer affecting males, and the second most diagnosed type of cancer affecting females, and one of the leading causes of cancer‑related mortality globally. The estimation of the micronuclei (MN) frequency in peripheral blood lymphocytes (PBLs) from patients with CRC is proposed as a prognostic/predictive easy‑to‑use biomarker. In this study, we aimed to investigate the effects of systemic treatment on the MN frequency in PBLs from patients with CRC in order to determine the effectiveness of the MN frequency as a biomarker. For this purpose, from 2016 to 2018, we quantified the MN frequency as a prognostic/predictive biomarker in serial samples from 25 patients with metastatic CRC (mCRC) using cytokinesis block micronucleus assay (CBMN assay). The MN frequency in the PBLs of the patients was evaluated before, during the middle and at the end of the therapy (approximately 0, 3 and 6 months). The results revealed a common pattern regarding the fluctuation in the MN frequency. Statistical analysis confirmed that when the disease response was estimated with radiological criteria, a good response was depicted at the MN frequency and vice versa. Consequently, the findings of this study suggest that the MN frequency may serve as a promising prognostic/predictive biomarker for the monitoring of the treatment response of patients with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Nikolouzakis TK, Stivaktakis PD, Apalaki P, Kalliantasi K, Sapsakos TM, Spandidos DA, Tsatsakis A, Souglakos J and Tsiaoussis J: Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer. Oncol Lett 17: 2703-2712, 2019.
APA
Nikolouzakis, T.K., Stivaktakis, P.D., Apalaki, P., Kalliantasi, K., Sapsakos, T.M., Spandidos, D.A. ... Tsiaoussis, J. (2019). Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer. Oncology Letters, 17, 2703-2712. https://doi.org/10.3892/ol.2019.9895
MLA
Nikolouzakis, T. K., Stivaktakis, P. D., Apalaki, P., Kalliantasi, K., Sapsakos, T. M., Spandidos, D. A., Tsatsakis, A., Souglakos, J., Tsiaoussis, J."Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer". Oncology Letters 17.3 (2019): 2703-2712.
Chicago
Nikolouzakis, T. K., Stivaktakis, P. D., Apalaki, P., Kalliantasi, K., Sapsakos, T. M., Spandidos, D. A., Tsatsakis, A., Souglakos, J., Tsiaoussis, J."Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer". Oncology Letters 17, no. 3 (2019): 2703-2712. https://doi.org/10.3892/ol.2019.9895
Copy and paste a formatted citation
x
Spandidos Publications style
Nikolouzakis TK, Stivaktakis PD, Apalaki P, Kalliantasi K, Sapsakos TM, Spandidos DA, Tsatsakis A, Souglakos J and Tsiaoussis J: Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer. Oncol Lett 17: 2703-2712, 2019.
APA
Nikolouzakis, T.K., Stivaktakis, P.D., Apalaki, P., Kalliantasi, K., Sapsakos, T.M., Spandidos, D.A. ... Tsiaoussis, J. (2019). Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer. Oncology Letters, 17, 2703-2712. https://doi.org/10.3892/ol.2019.9895
MLA
Nikolouzakis, T. K., Stivaktakis, P. D., Apalaki, P., Kalliantasi, K., Sapsakos, T. M., Spandidos, D. A., Tsatsakis, A., Souglakos, J., Tsiaoussis, J."Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer". Oncology Letters 17.3 (2019): 2703-2712.
Chicago
Nikolouzakis, T. K., Stivaktakis, P. D., Apalaki, P., Kalliantasi, K., Sapsakos, T. M., Spandidos, D. A., Tsatsakis, A., Souglakos, J., Tsiaoussis, J."Effect of systemic treatment on the micronuclei frequency in the peripheral blood of patients with metastatic colorectal cancer". Oncology Letters 17, no. 3 (2019): 2703-2712. https://doi.org/10.3892/ol.2019.9895
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