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Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma

  • Authors:
    • Maarouf A. Saad
    • Jonjei Ku
    • Selena Z. Kuo
    • Pin Xue Li
    • Hao Zheng
    • Michael Andrew Yu
    • Jessica Wang‑Rodriguez
    • Weg M. Ongkeko
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Yale University, New Haven, CT 06510, USA, Division of Otolaryngology‑Head and Neck Surgery, Department of Surgery, University of California, San Diego, La Jolla, CA 92093, USA, School of Medicine, University of California, San Francisco, CA 94143, USA, Veterans Administration Medical Center and Department of Pathology, University of California, San Diego, La Jolla, CA 92161, USA
    Copyright: © Saad et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2615-2622
    |
    Published online on: January 9, 2019
       https://doi.org/10.3892/ol.2019.9913
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Abstract

It is clear that alcohol consumption is a major risk factor in the pathogenesis of head and neck squamous cell carcinoma (HNSCC); however, the molecular mechanism underlying the pathogenesis of alcohol‑associated HNSCC remains poorly understood. The aim of the present study was to identify and characterize P‑element‑induced wimpy testis (PIWI)‑interacting RNAs (piRNAs) and PIWI proteins dysregulated in alcohol‑associated HNSCC to elucidate their function in the development of this cancer. Using next generation RNA‑sequencing (RNA‑seq) data obtained from 40 HNSCC patients, the piRNA and PIWI protein expression of HNSCC samples was compared between alcohol drinkers and non‑drinkers. A separate piRNA expression RNA‑seq analysis of 18 non‑smoker HNSCC patients was also conducted. To verify piRNA expression, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed on the most differentially expressed alcohol‑associated piRNAs in ethanol and acetaldehyde‑treated normal oral keratinocytes. The correlation between piRNA expression and patient survival was analyzed using Kaplan‑Meier estimators and multivariate Cox proportional hazard models. A comparison between alcohol drinking and non‑drinking HNSCC patients demonstrated that a panel of 3,223 piRNA transcripts were consistently detected and differentially expressed. RNA‑seq analysis and in vitro RT‑qPCR verifica­tion revealed that 4 of these piRNAs, piR‑35373, piR‑266308, piR‑58510 and piR‑38034, were significantly dysregulated between drinking and non‑drinking cohorts. Of these four piRNAs, low expression of piR‑58510 and piR‑35373 significantly correlated with improved patient survival. Furthermore, human PIWI‑like protein 4 was consistently upregulated in ethanol and acetaldehyde‑treated normal oral keratinocytes. These results demonstrate that alcohol consumption may cause dysregulation of piRNA expression in HNSCC and in vitro verifications identified 4 piRNAs that may be involved in the pathogenesis of alcohol‑associated HNSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Saad MA, Ku J, Kuo SZ, Li PX, Zheng H, Yu MA, Wang‑Rodriguez J and Ongkeko WM: Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma. Oncol Lett 17: 2615-2622, 2019.
APA
Saad, M.A., Ku, J., Kuo, S.Z., Li, P.X., Zheng, H., Yu, M.A. ... Ongkeko, W.M. (2019). Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma. Oncology Letters, 17, 2615-2622. https://doi.org/10.3892/ol.2019.9913
MLA
Saad, M. A., Ku, J., Kuo, S. Z., Li, P. X., Zheng, H., Yu, M. A., Wang‑Rodriguez, J., Ongkeko, W. M."Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma". Oncology Letters 17.3 (2019): 2615-2622.
Chicago
Saad, M. A., Ku, J., Kuo, S. Z., Li, P. X., Zheng, H., Yu, M. A., Wang‑Rodriguez, J., Ongkeko, W. M."Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma". Oncology Letters 17, no. 3 (2019): 2615-2622. https://doi.org/10.3892/ol.2019.9913
Copy and paste a formatted citation
x
Spandidos Publications style
Saad MA, Ku J, Kuo SZ, Li PX, Zheng H, Yu MA, Wang‑Rodriguez J and Ongkeko WM: Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma. Oncol Lett 17: 2615-2622, 2019.
APA
Saad, M.A., Ku, J., Kuo, S.Z., Li, P.X., Zheng, H., Yu, M.A. ... Ongkeko, W.M. (2019). Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma. Oncology Letters, 17, 2615-2622. https://doi.org/10.3892/ol.2019.9913
MLA
Saad, M. A., Ku, J., Kuo, S. Z., Li, P. X., Zheng, H., Yu, M. A., Wang‑Rodriguez, J., Ongkeko, W. M."Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma". Oncology Letters 17.3 (2019): 2615-2622.
Chicago
Saad, M. A., Ku, J., Kuo, S. Z., Li, P. X., Zheng, H., Yu, M. A., Wang‑Rodriguez, J., Ongkeko, W. M."Identification and characterization of dysregulated P‑element induced wimpy testis‑interacting RNAs in head and neck squamous cell carcinoma". Oncology Letters 17, no. 3 (2019): 2615-2622. https://doi.org/10.3892/ol.2019.9913
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