Open Access

SOX5 promotes breast cancer proliferation and invasion by transactivation of EZH2

  • Authors:
    • Chuntao Sun
    • Yunqing Ban
    • Kai Wang
    • Yanming Sun
    • Zhihua Zhao
  • View Affiliations

  • Published online on: January 9, 2019     https://doi.org/10.3892/ol.2019.9914
  • Pages: 2754-2762
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Sex determining region Y‑box protein 5 (SOX5) is a transcriptional factor and serves important roles in various cancer types; however, the pathological role of SOX5 in patients with breast cancer remains unclear. In the present study, the expression and potential role of SOX5 in patients with breast cancer and in breast cancer cells was investigated. The data indicated that SOX5 was highly expressed in breast cancer tissues compared with adjacent healthy tissues, and overexpression of SOX5 was associated with a reduced overall survival rate in patients with breast cancer. Gain and loss of function studies with MTT, colony formation, wound healing and Matrigel invasion assays demonstrated that SOX5 significantly promoted breast cancer cell proliferation and invasion. The chromatin immunoprecipitation (ChIP) assay sequence, quantitative ChIP and luciferase reporter assays were used to identify enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) as a downstream target gene of SOX5. Furthermore, it was determined that ectopic expression of SOX5 increased EZH2 expression at the mRNA and protein level, while the knockdown of SOX5 decreased EZH2 expression. Additionally, the biological effect of SOX5 was investigated, and it was determined to be dependent on the regulation of EZH2 expression. The present results may provide important insights into the biological significance of SOX5 serving as a candidate therapeutic target in breast cancer progression.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Sun C, Ban Y, Wang K, Sun Y and Zhao Z: SOX5 promotes breast cancer proliferation and invasion by transactivation of EZH2. Oncol Lett 17: 2754-2762, 2019.
APA
Sun, C., Ban, Y., Wang, K., Sun, Y., & Zhao, Z. (2019). SOX5 promotes breast cancer proliferation and invasion by transactivation of EZH2. Oncology Letters, 17, 2754-2762. https://doi.org/10.3892/ol.2019.9914
MLA
Sun, C., Ban, Y., Wang, K., Sun, Y., Zhao, Z."SOX5 promotes breast cancer proliferation and invasion by transactivation of EZH2". Oncology Letters 17.3 (2019): 2754-2762.
Chicago
Sun, C., Ban, Y., Wang, K., Sun, Y., Zhao, Z."SOX5 promotes breast cancer proliferation and invasion by transactivation of EZH2". Oncology Letters 17, no. 3 (2019): 2754-2762. https://doi.org/10.3892/ol.2019.9914