MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN

  • Authors:
    • Jing He
    • Mimi Mu
    • Yulan Luo
    • Helong Wang
    • Hua Ma
    • Shujun Guo
    • Qiang Fang
    • Zhongqing Qian
    • Hezuo Lu
    • Chuanwang Song
  • View Affiliations

  • Published online on: January 14, 2019     https://doi.org/10.3892/ol.2019.9925
  • Pages: 2931-2936
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Abstract

MicroRNAs (miRNAs/miRs) are small, noncoding RNA molecules that are closely associated with the occurrence and development of tumors. miR‑20b is overexpressed in hepatocellular carcinoma cell lines and tissues. However, it is not clear whether miR‑20b can promote the proliferation of hepatocellular carcinoma cells. In the present study, the proliferation of H22 mouse hepatocellular carcinoma cells was detected using the Cell Counting Kit‑8 assay. MiRanda software was used to predict the binding sites of miR‑20b to the 3'‑untranslated region (3'‑UTR) of phosphatase and tensin homolog (PTEN). The 3'‑UTR sequence of the PTEN gene was amplified using the polymerase chain reaction in H22 cells. The recombinant plasmid or empty plasmid was co‑transfected with miR‑20b mimics or miR‑20b scramble into HeLa cells, and luciferase activity was assessed by Dual‑Luciferase® Reporter Assay System 24 h post‑transfection. In the present study, miR‑20b knockdown significantly inhibited the proliferation of H22 mouse hepatocellular carcinoma cells. In addition, miR‑20b inhibition upregulated the expression of PTEN, and it was revealed that miR‑20b may directly target the 3'‑untranslated region of the PTEN gene. Downregulation of PTEN partially reversed the anti‑proliferative effect of miR‑20b on H22 cells. In conclusion, miR‑20b may promote H22 cell proliferation by targeting PTEN, providing a rationale for further study investigating novel therapeutic strategies for liver cancer.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
He J, Mu M, Luo Y, Wang H, Ma H, Guo S, Fang Q, Qian Z, Lu H, Song C, Song C, et al: MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN. Oncol Lett 17: 2931-2936, 2019
APA
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S. ... Song, C. (2019). MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN. Oncology Letters, 17, 2931-2936. https://doi.org/10.3892/ol.2019.9925
MLA
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S., Fang, Q., Qian, Z., Lu, H., Song, C."MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN". Oncology Letters 17.3 (2019): 2931-2936.
Chicago
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S., Fang, Q., Qian, Z., Lu, H., Song, C."MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN". Oncology Letters 17, no. 3 (2019): 2931-2936. https://doi.org/10.3892/ol.2019.9925