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Article

MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN

  • Authors:
    • Jing He
    • Mimi Mu
    • Yulan Luo
    • Helong Wang
    • Hua Ma
    • Shujun Guo
    • Qiang Fang
    • Zhongqing Qian
    • Hezuo Lu
    • Chuanwang Song
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Microbiology and Parasitology, Bengbu Medical College, and Anhui Key Laboratory of Infection and Immunity at Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
  • Pages: 2931-2936
    |
    Published online on: January 14, 2019
       https://doi.org/10.3892/ol.2019.9925
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Abstract

MicroRNAs (miRNAs/miRs) are small, noncoding RNA molecules that are closely associated with the occurrence and development of tumors. miR‑20b is overexpressed in hepatocellular carcinoma cell lines and tissues. However, it is not clear whether miR‑20b can promote the proliferation of hepatocellular carcinoma cells. In the present study, the proliferation of H22 mouse hepatocellular carcinoma cells was detected using the Cell Counting Kit‑8 assay. MiRanda software was used to predict the binding sites of miR‑20b to the 3'‑untranslated region (3'‑UTR) of phosphatase and tensin homolog (PTEN). The 3'‑UTR sequence of the PTEN gene was amplified using the polymerase chain reaction in H22 cells. The recombinant plasmid or empty plasmid was co‑transfected with miR‑20b mimics or miR‑20b scramble into HeLa cells, and luciferase activity was assessed by Dual‑Luciferase® Reporter Assay System 24 h post‑transfection. In the present study, miR‑20b knockdown significantly inhibited the proliferation of H22 mouse hepatocellular carcinoma cells. In addition, miR‑20b inhibition upregulated the expression of PTEN, and it was revealed that miR‑20b may directly target the 3'‑untranslated region of the PTEN gene. Downregulation of PTEN partially reversed the anti‑proliferative effect of miR‑20b on H22 cells. In conclusion, miR‑20b may promote H22 cell proliferation by targeting PTEN, providing a rationale for further study investigating novel therapeutic strategies for liver cancer.
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1 

de Martel C, Maucort-Boulch D, Plummer M and Franceschi S: World-wide relative contribution of hepatitis B and C viruses in hepatocellular carcinoma. Hepatology. 62:1190–1200. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

3 

He RQ, Wu PR, Xiang XL, Yang X, Liang HW, Qiu XH, Yang LH, Peng ZG and Chen G: Downregulated miR-23b-3p expression acts as a predictor of hepatocellular carcinoma progression: A study based on public data and RT-qPCR verification. Int J Mol Med. 41:2813–2831. 2018.PubMed/NCBI

4 

M'Bengue AK, Doumbia M, Denoman SR, Ouattara DN, Adoubi I and Pineau P: A major shift of viral and nutritional risk factors affects the hepatocellular carcinoma risk among Ivorian patients: A preliminary report. Infect Agent Cancer. 10:182015. View Article : Google Scholar : PubMed/NCBI

5 

Zhang E, Liu Q, Wang Y, Wang H, He L, Jin X and Li N: MicroRNA miR-147b promotes tumor growth via targeting UBE2N in hepatocellular carcinoma. Oncotarge. 8:114072–114080. 2017. View Article : Google Scholar

6 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D, Sweet-Cordero A, Ebert BL, Mak RH, Ferrando AA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Liu Z, Wang Y, Dou C, Sun L, Li Q, Wang L, Xu Q, Yang W, Liu Q and Tu K: MicroRNA-1468 promotes tumor progression by activating PPAR-γ-mediated AKT signaling in human hepatocellular carcinoma. J Exp Clin Cancer Res. 37:492018. View Article : Google Scholar : PubMed/NCBI

9 

Tanzer A and Stadler PF: Molecular evolution of a microRNA cluster. J Mol Biol. 339:327–335. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, Tomida S, Yatabe Y, Kawahara K, Sekido Y and Takahashi T: A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Res. 65:9628–9632. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Katada T, Ishiguro H, Kuwabara Y, Kimura M, Mitui A, Mori Y, Ogawa R, Harata K and Fujii Y: microRNA expression profile in undifferentiated gastric cancer. Int J Oncol. 34:537–542. 2009.PubMed/NCBI

12 

Guo J, Xiao Z, Yu X and Cao R: miR-20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer. Oncol Lett. 14:6895–6900. 2017.PubMed/NCBI

13 

Zhou W, Shi G, Zhang Q, Wu Q, Li B and Zhang Z: MicroRNA-20b promotes cell growth of breast cancer cells partly via targeting phosphatase and tensin homologue (PTEN). Cell Biosci. 4:622014. View Article : Google Scholar : PubMed/NCBI

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Song C, Ma H, Yao C, Tao X and Gan H: Alveolar macrophage-derived vascular endothelial growth factor contributes to allergic airway inflammation in a mouse asthma model. Scand J Immunol. 75:599–605. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Chen L and Guo D: The functions of tumor suppressor PTEN in innate and adaptive immunity. Cell Mol Immunol. 14:581–589. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Ghosh N and Katare R: Molecular mechanism of diabetic cardiomyopathy and modulation of microRNA function by synthetic oligonucleotides. Cardiovasc Diabetol. 17:432018. View Article : Google Scholar : PubMed/NCBI

18 

Xue TM, Tao LD, Zhang M, Zhang J, Liu X, Chen GF, Zhu YJ and Zhang PJ: Clinicopathological significance of microRNA-20b expression in hepatocellular carcinoma and regulation of HIF-1α and VEGF effect on cell biological behaviour. Dis Markers. 2015:3251762015. View Article : Google Scholar : PubMed/NCBI

19 

Xue TM, Tao LD, Zhang M, Xu GC, Zhang J and Zhang PJ: miR-20b overexpression is predictive of poor prognosis in gastric cancer. Onco Targets Ther. 8:1871–1876. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Ahmad A, Ginnebaugh KR, Sethi S, Chen W, Ali R, Mittal S and Sarkar FH: miR-20b is up-regulated in brain metastases from primary breast cancers. Oncotarget. 6:12188–12195. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Leidinger P, Brefort T, Backes C, Krapp M, Galata V, Beier M, Kohlhaas J, Huwer H, Meese E and Keller A: High-throughput qRT-PCR validation of blood microRNAs in non-small cell lung cancer. Oncotarget. 7:4611–4623. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Borges NM, do Vale Elias M, Fook-Alves VL, Andrade TA, de Conti ML, Macedo MP, Begnami MD, Campos AH, Etto LY, Bortoluzzo AB, et al: Angiomirs expression profiling in diffuse large B-Cell lymphoma. Oncotarget. 7:4806–4816. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang SI, Puc J, Miliaresis C, Rodgers L, McCombie R, et al: PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science. 275:1943–1947. 1997. View Article : Google Scholar : PubMed/NCBI

24 

Bertram J, Peacock JW, Tan C, Mui AL, Chung SW, Gleave ME, Dedhar S, Cox ME and Ong CJ: Inhibition of the phosphatidylinositol 3′-kinase pathway promotes autocrine Fas-induced death of phosphatase and tensin homologue-deficient prostate cancer cells. Cancer Res. 66:4781–4788. 2006. View Article : Google Scholar : PubMed/NCBI

25 

Meng F, Henson R, Wehbe-Janek H, Ghoshal K, Jacob ST and Patel T: MicroRNA-21 regulates expression of the PTEN tumor suppressor gene in human hepatocellular cancer. Gastroenterology. 133:647–658. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Chung JH, Ginn-Pease ME and Eng C: Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) has nuclear localization signal-like sequences for nuclear import mediated by major vault protein. Cancer Res. 65:4108–4116. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Hjelmeland AB, Hjelmeland MD, Shi Q, Hart JL, Bigner DD, Wang XF, Kontos CD and Rich JN: Loss of phosphatase and tensin homologue increases transforming growth factor beta-mediated invasion with enhanced SMAD3 transcriptional activity. Cancer Res. 65:11276–11281. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Yang H, Kong W, He L, Zhao JJ, O'Donnell JD, Wang J, Wenham RM, Coppola D, Kruk PA, Nicosia SV and Cheng JQ: MicroRNA expression profiling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN. Cancer Res. 68:425–433. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Wu W, Yang J, Feng X, Wang H, Ye S, Yang P, Tan W, Wei G and Zhou Y: MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth, migration, and invasion in colorectal carcinoma cells. Mol Cancer. 12:302013. View Article : Google Scholar : PubMed/NCBI

30 

Huse JT, Brennan C, Hambardzumyan D, Wee B, Pena J, Rouhanifard SH, Sohn-Lee C, le Sage C, Agami R, Tuschl T and Holland EC: The PTEN-regulating microRNA miR-26a is amplified in high-grade glioma and facilitates gliomagenesis in vivo. Genes Dev. 23:1327–1337. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Dai X, Fang M, Li S, Yan Y, Zhong Y and Du B: miR-21 is involved in transforming growth factor β1-induced chemoresistance and invasion by targeting PTEN in breast cancer. Oncol Lett. 14:6929–6936. 2017.PubMed/NCBI

32 

Chu P, Liang A, Jiang A and Zong L: miR-205 regulates the proliferation and invasion of ovarian cancer cells via suppressing PTEN/SMAD4 expression. Oncol Lett. 15:7571–7578. 2018.PubMed/NCBI

33 

Luo B, Kang N, Chen Y, Liu L and Zhang Y: Oncogene miR-106a promotes proliferation and metastasis of prostate cancer cells by directly targeting PTEN in vivo and in vitro. Minerva Med. 109:24–30. 2018.PubMed/NCBI

34 

Chai Y1, Xiaoyu L and Haiyan W: Correlation between expression levels of PTEN and p53 genes and the clinical features of HBsAg-positive liver cancer. J BUON. 22:942–946. 2017.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
He J, Mu M, Luo Y, Wang H, Ma H, Guo S, Fang Q, Qian Z, Lu H, Song C, Song C, et al: MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN. Oncol Lett 17: 2931-2936, 2019.
APA
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S. ... Song, C. (2019). MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN. Oncology Letters, 17, 2931-2936. https://doi.org/10.3892/ol.2019.9925
MLA
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S., Fang, Q., Qian, Z., Lu, H., Song, C."MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN". Oncology Letters 17.3 (2019): 2931-2936.
Chicago
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S., Fang, Q., Qian, Z., Lu, H., Song, C."MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN". Oncology Letters 17, no. 3 (2019): 2931-2936. https://doi.org/10.3892/ol.2019.9925
Copy and paste a formatted citation
x
Spandidos Publications style
He J, Mu M, Luo Y, Wang H, Ma H, Guo S, Fang Q, Qian Z, Lu H, Song C, Song C, et al: MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN. Oncol Lett 17: 2931-2936, 2019.
APA
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S. ... Song, C. (2019). MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN. Oncology Letters, 17, 2931-2936. https://doi.org/10.3892/ol.2019.9925
MLA
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S., Fang, Q., Qian, Z., Lu, H., Song, C."MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN". Oncology Letters 17.3 (2019): 2931-2936.
Chicago
He, J., Mu, M., Luo, Y., Wang, H., Ma, H., Guo, S., Fang, Q., Qian, Z., Lu, H., Song, C."MicroRNA‑20b promotes proliferation of H22 hepatocellular carcinoma cells by targeting PTEN". Oncology Letters 17, no. 3 (2019): 2931-2936. https://doi.org/10.3892/ol.2019.9925
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