Knockdown of high mobility group box 3 impairs cell viability and colony formation but increases apoptosis in A549 human non‑small cell lung cancer cells

  • Authors:
    • Ning Song
    • Baohua Wang
    • Guishan Feng
    • Lin Duan
    • Shengfang Yuan
    • Weihua Jia
    • Yi Liu
  • View Affiliations

  • Published online on: January 14, 2019     https://doi.org/10.3892/ol.2019.9927
  • Pages: 2937-2945
Metrics: HTML 0 views | PDF 0 views     Cited By (CrossRef): 0 citations

Abstract

Previous research has linked high mobility group box 3 (HMGB3) overexpression to the malignant progression and poor prognosis of non‑small cell lung cancer (NSCLC). The present study investigated the role of HMGB3 in cell survival and colony formation of NSCLC cells. Stable knockdown of HMGB3 in A549 cells was achieved by lentiviral‑based shRNA interference and verified by detection of the transcriptional and translational level of HMGB3 with reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. The influence of HMGB3 knockdown on A549 cell viability and apoptotic rate was evaluated by Cell Counting Kit‑8 assay and flow cytometry following annexin V staining, respectively. The proliferative capacity of A549 cells with or without HMGB3 knockdown was compared by measuring their colony forming efficiency. The results of the current study revealed that HMGB3 knockdown significantly reduced cell viability and colony forming efficiency while promoting apoptosis in A549 cells, indicating that HMGB3 may be pivotal for the survival and colony formation of A549 cells, serving a notable role in NSCLC progression.

Related Articles

Journal Cover

March 2019
Volume 17 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Song, N., Wang, B., Feng, G., Duan, L., Yuan, S., Jia, W., & Liu, Y. (2019). Knockdown of high mobility group box 3 impairs cell viability and colony formation but increases apoptosis in A549 human non‑small cell lung cancer cells. Oncology Letters, 17, 2937-2945. https://doi.org/10.3892/ol.2019.9927
MLA
Song, N., Wang, B., Feng, G., Duan, L., Yuan, S., Jia, W., Liu, Y."Knockdown of high mobility group box 3 impairs cell viability and colony formation but increases apoptosis in A549 human non‑small cell lung cancer cells". Oncology Letters 17.3 (2019): 2937-2945.
Chicago
Song, N., Wang, B., Feng, G., Duan, L., Yuan, S., Jia, W., Liu, Y."Knockdown of high mobility group box 3 impairs cell viability and colony formation but increases apoptosis in A549 human non‑small cell lung cancer cells". Oncology Letters 17, no. 3 (2019): 2937-2945. https://doi.org/10.3892/ol.2019.9927