Open Access

Diagnosis of hepatocellular carcinoma using a novel anti‑glycocholic acid monoclonal antibody‑based method

  • Authors:
    • Miao Wu
    • Songhui Zhai
    • Julia Gao
    • Dapeng Wei
    • Jianxin Xue
    • Yuxi Zhou
    • Nan Li
    • Lijuan Hu
  • View Affiliations

  • Published online on: January 17, 2019     https://doi.org/10.3892/ol.2019.9943
  • Pages: 3103-3112
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glycocholic acid (GCA) is a novel identified biomarker for hepatocellular carcinoma (HCC). However, clinical pathological study of GCA has not been extensive due to the limited availability of anti‑GCA monoclonal antibodies (mAbs) and restricted detection methods. In the present study, using human GCA conjugated with bovine serum albumin as the immunogen to immunize BALB/c mice, a novel anti‑GCA mAb was generated and characterized. The isotypes of heavy chain and light chain of anti‑GCA mAb were examined to be IgG2a and κ, respectively, with a high affinity constant (2.6x108 mol/l). The anti‑GCA mAb binds GCA with high specificity and sensitivity, and the 50% inhibitory rate was 77.09 ng/ml. The present study also established a rapid, sensitive and efficient indirect competitive ELISA analysis using this anti‑GCA mAb to detect the level of GCA produced by different HCC cell lines. Therefore, the present study may successfully develop a novel method for early HCC diagnosis, and also provide insights for further research and treatment of HCC.
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March-2019
Volume 17 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wu M, Zhai S, Gao J, Wei D, Xue J, Zhou Y, Li N and Hu L: Diagnosis of hepatocellular carcinoma using a novel anti‑glycocholic acid monoclonal antibody‑based method. Oncol Lett 17: 3103-3112, 2019
APA
Wu, M., Zhai, S., Gao, J., Wei, D., Xue, J., Zhou, Y. ... Hu, L. (2019). Diagnosis of hepatocellular carcinoma using a novel anti‑glycocholic acid monoclonal antibody‑based method. Oncology Letters, 17, 3103-3112. https://doi.org/10.3892/ol.2019.9943
MLA
Wu, M., Zhai, S., Gao, J., Wei, D., Xue, J., Zhou, Y., Li, N., Hu, L."Diagnosis of hepatocellular carcinoma using a novel anti‑glycocholic acid monoclonal antibody‑based method". Oncology Letters 17.3 (2019): 3103-3112.
Chicago
Wu, M., Zhai, S., Gao, J., Wei, D., Xue, J., Zhou, Y., Li, N., Hu, L."Diagnosis of hepatocellular carcinoma using a novel anti‑glycocholic acid monoclonal antibody‑based method". Oncology Letters 17, no. 3 (2019): 3103-3112. https://doi.org/10.3892/ol.2019.9943