Correlation the between the regulation of miRNA‑1 in c‑Met‑induced EMT and cervical cancer progression

Cheng,Yun; Yang,Minliang; Peng,Jingxian

doi:10.3892/ol.2019.9971

Correlation the between the regulation of miRNA‑1 in c‑Met‑induced EMT and cervical cancer progression

Authors:
- Yun Cheng
- Minliang Yang
- Jingxian Peng
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Affiliations: Department of Histology and Embryology, School of Preclinical and Forensic Medicine, Baotou Medical College, Baotou, Inner Mongolia 014000, P.R. China, Medical Ultrasound Center, Northwest Women's and Children's Hospital, Xian, Shanxi 710000, P.R. China, Microbiology Laboratory, Baotou Center for Disease Control and Prevention, Baotou, Inner Mongolia 014000, P.R. China
Published online on: January 25, 2019 https://doi.org/10.3892/ol.2019.9971
Pages: 3341-3349
Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cervical cancer is a common malignant tumor of the female reproductive system. Despite advances in cervical cancer therapy, tumor recurrence and metastasis remain the leading cause of mortality for patients with cervical cancer. Therefore, the investigation of tumorigenesis and progression, and the search for novel therapeutic targets, has been the primary focus in cervical cancer research. The aims of the present study were: i) To analyze the alterations in c‑Met, E‑cadherin and microRNA (miRNA)‑1 expression levels in cervical cancer tissues; ii) to assess the correlation between the above genes and the pathological characteristics of the cancer tissues; and iii) to examine the potential mechanism through which miRNA‑1 may regulate c‑Met‑induced epithelial‑mesenchymal transition to promote the development of cervical cancer. In cervical cancer tissues, c‑Met was more highly expressed, while E‑cadherin exhibited lower expression levels compared with the adjacent tissues. The 24‑month follow‑up reported that a lower c‑Met expression level was correlated with higher E‑cadherin expression levels and a longer survival rate. The miRNA‑1 expression level in cancer tissues was 0.41±0.07 times lower compared with the adjacent tissues (P<0.01). A low miRNA expression level was correlated with a low survival rate of patients. In vitro, miRNA‑1 inhibited the proliferation and migration of cervical cancer cell lines by downregulating c‑Met mRNA. When miRNA‑1 expression was downregulated in cervical cancer tissues, the inhibition of c‑Met expression was reversed. The upregulation of c‑Met expression levels was able to inhibit E‑cadherin expression, which triggered the proliferation, migration and infiltration of cancer cells, and thus reduced patient survival rates.

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