Open Access

Phosphorylated STAT3 expression linked to SOCS3 methylation is associated with proliferative ability of gastric mucosa in patients with early gastric cancer

  • Authors:
    • Hirokazu Fukui
    • Jiro Watari
    • Xinxing Zhang
    • Ying Ran
    • Toshihiko Tomita
    • Tadayuki Oshima
    • Seiichi Hirota
    • Hiroto Miwa
  • View Affiliations

  • Published online on: March 16, 2020     https://doi.org/10.3892/ol.2020.11462
  • Pages: 3542-3550
  • Copyright: © Fukui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Gastric cancers (GCs) may develop in the gastric mucosa after elimination of Helicobacter pylori (H. pylori) using eradication therapy. Cytokine signaling is a key mechanism underlying GC development and progression, and STAT3 signaling may serve a central role in gastritis‑associated tumorigenesis. In the present study, suppressor of cytokine signaling 3 (SOCS3) methylation was examined, as an activator of phosphorylated (p‑)STAT3 expression in the non‑neoplastic gastric mucosa (non‑NGM) of patients with early GC. The methylation status of the SOCS3 gene promoter was analyzed using methylation‑specific PCR in the non‑NGM of patients with or without early GC. Expression levels of p‑STAT3 and Ki67 were investigated immunohistochemically in non‑NGM with early GC before and after H. pylori eradication. In non‑NGM, SOCS3 promoter methylation was detected in 17/51 patients (33.3%) with early GC. In those patients, the non‑NGM labeling indices of both Ki67 and p‑STAT3 were significantly higher compared with that in patients with early GC without SOCS3 methylation. A significant correlation between Ki67 and p‑STAT3 expression levels was demonstrated in the non‑NGM of patients with early GC. In patients with early GC without SOCS3 methylation, the labeling indices of both Ki67 and p‑STAT3 in non‑NGM were significantly reduced after H. pylori eradication, whereas no such change was observed in patients with early GC with SOCS3 methylation. SOCS3 methylation is associated with continuous p‑STAT3 overexpression and enhanced epithelial cell proliferation in non‑NGM of patients with early GC.

Related Articles

Journal Cover

May 2020
Volume 19 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Fukui, H., Watari, J., Zhang, X., Ran, Y., Tomita, T., Oshima, T. ... Miwa, H. (2020). Phosphorylated STAT3 expression linked to SOCS3 methylation is associated with proliferative ability of gastric mucosa in patients with early gastric cancer. Oncology Letters, 19, 3542-3550. https://doi.org/10.3892/ol.2020.11462
MLA
Fukui, H., Watari, J., Zhang, X., Ran, Y., Tomita, T., Oshima, T., Hirota, S., Miwa, H."Phosphorylated STAT3 expression linked to SOCS3 methylation is associated with proliferative ability of gastric mucosa in patients with early gastric cancer". Oncology Letters 19.5 (2020): 3542-3550.
Chicago
Fukui, H., Watari, J., Zhang, X., Ran, Y., Tomita, T., Oshima, T., Hirota, S., Miwa, H."Phosphorylated STAT3 expression linked to SOCS3 methylation is associated with proliferative ability of gastric mucosa in patients with early gastric cancer". Oncology Letters 19, no. 5 (2020): 3542-3550. https://doi.org/10.3892/ol.2020.11462