Open Access

Expression and regulation effects of chemokine receptor 7 in colon cancer cells

  • Authors:
    • Xiang Li
    • Xuemei Wang
    • Zitao Li
    • Yanjun Liu
    • Liang Sang
    • Zhen Zhang
    • Yixia Zhang
  • View Affiliations

  • Published online on: April 21, 2020     https://doi.org/10.3892/ol.2020.11561
  • Pages: 226-234
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In China the incidence and mortality rates of colon cancer have been increasing annually. Studies have revealed that CXCR7 is expressed in many tumors. The aim of the present study was to investigate the function of CXCR7 in colon cancer. The expression level of chemokine receptor 7 (CXCR7) in Caco‑2 and HCT116 cells was investigated to elucidate the effect of CXCR7 on cell biological behavior. Reverse transcription‑quantitative PCR and western blot analysis were used to detect the expression level of CXCR7 in Caco‑2 and HCT116 cells after transfection with small interfering (si)RNA. To analyze the in vitro biological function of CXCR7, cell proliferation was measured using a Cell Counting Kit‑8 assay, and cell invasion and migration were measured using Matrigel, and Transwell and wound healing assays. siRNAs were successfully transfected into Caco‑2 and HCT116 cells and resulted in a decrease in CXCR7 protein and mRNA expression. Downregulation of CXCR7 inhibited Caco‑2 and HCT116 cell proliferation, invasion, and migration. Regulation of CXCR7 expression may affect the biological behavior of Caco‑2 and HCT116 cells, suggesting that CXCR7 has a potential role in molecular therapy in colon cancer.

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July 2020
Volume 20 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Li, X., Wang, X., Li, Z., Liu, Y., Sang, L., Zhang, Z., & Zhang, Y. (2020). Expression and regulation effects of chemokine receptor 7 in colon cancer cells. Oncology Letters, 20, 226-234. https://doi.org/10.3892/ol.2020.11561
MLA
Li, X., Wang, X., Li, Z., Liu, Y., Sang, L., Zhang, Z., Zhang, Y."Expression and regulation effects of chemokine receptor 7 in colon cancer cells". Oncology Letters 20.1 (2020): 226-234.
Chicago
Li, X., Wang, X., Li, Z., Liu, Y., Sang, L., Zhang, Z., Zhang, Y."Expression and regulation effects of chemokine receptor 7 in colon cancer cells". Oncology Letters 20, no. 1 (2020): 226-234. https://doi.org/10.3892/ol.2020.11561