Open Access

Fat mass and obesity-associated protein promotes the tumorigenesis and development of liver cancer

  • Authors:
    • Ziqi Ye
    • Shibing Wang
    • Wanyuan Chen
    • Xin Zhang
    • Jie Chen
    • Jinying Jiang
    • Mingshan Wang
    • Li Zhang
    • Zixue Xuan
  • View Affiliations

  • Published online on: May 25, 2020     https://doi.org/10.3892/ol.2020.11673
  • Pages: 1409-1417
  • Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Liver cancer is the fourth leading cause of cancer‑associated mortality worldwide. Statistics indicate that the incidence of liver cancer has been increasing and that its prognosis remains poor. Fat mass and obesity‑associated protein (FTO) is a demethylase that is involved in N6‑methyladenosine (m6a) RNA modification; however, to the best of our knowledge, its role in tumorigenesis and development of liver cancer remains unknown. In the present study, cell proliferation, colony formation, apoptosis, Transwell and wound healing assays of small interfering (si)RNA‑FTO HepG2 cells were performed, and the levels of m6A RNA methylation were assessed. Additionally, the prognostic value of FTO in liver cancer was analyzed using immunohistochemistry analysis. The results from the EpiQuik m6A RNA methylation quantitative assay revealed that knockdown of FTO increased the total m6A methylation level. Notably, FTO promoted the proliferation and migration of liver cancer cells. Additionally, FTO expression was upregulated in patients with liver cancer and was associated with a high Edmondson Grade, which served as an independent prognostic factor for liver cancer. Results from the Kaplan‑Meier survival analysis revealed that low expression levels of FTO predicted a good prognosis. The 5‑year overall survival of the low FTO expression group was 68% compared with 48% in the high FTO expression group (P=0.077). In conclusion, the present study suggested that FTO regulates the tumorigenesis and development of liver cancer.

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August-2020
Volume 20 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Ye Z, Wang S, Chen W, Zhang X, Chen J, Jiang J, Wang M, Zhang L and Xuan Z: Fat mass and obesity-associated protein promotes the tumorigenesis and development of liver cancer . Oncol Lett 20: 1409-1417, 2020
APA
Ye, Z., Wang, S., Chen, W., Zhang, X., Chen, J., Jiang, J. ... Xuan, Z. (2020). Fat mass and obesity-associated protein promotes the tumorigenesis and development of liver cancer . Oncology Letters, 20, 1409-1417. https://doi.org/10.3892/ol.2020.11673
MLA
Ye, Z., Wang, S., Chen, W., Zhang, X., Chen, J., Jiang, J., Wang, M., Zhang, L., Xuan, Z."Fat mass and obesity-associated protein promotes the tumorigenesis and development of liver cancer ". Oncology Letters 20.2 (2020): 1409-1417.
Chicago
Ye, Z., Wang, S., Chen, W., Zhang, X., Chen, J., Jiang, J., Wang, M., Zhang, L., Xuan, Z."Fat mass and obesity-associated protein promotes the tumorigenesis and development of liver cancer ". Oncology Letters 20, no. 2 (2020): 1409-1417. https://doi.org/10.3892/ol.2020.11673