Open Access

miR‑502‑5p inhibits the proliferation, migration and invasion of gastric cancer cells by targeting SP1

  • Authors:
    • Xiaobo Peng
    • Meihong Wu
    • Wuxia Liu
    • Chengtao Guo
    • Lixing Zhan
    • Xianbao Zhan
  • View Affiliations

  • Published online on: July 3, 2020     https://doi.org/10.3892/ol.2020.11808
  • Pages: 2757-2762
  • Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is the third most common cause of cancer‑associated mortality in China. Aberrant microRNA (miR) expression can occur through multiple biological processes and has been implicated in cancer development. However, to the best of our knowledge, the function of miR‑502‑5p in GC is currently unclear. In the present study, the expression and function of miR‑502‑5p in GC was evaluated. Reverse transcription‑quantitative (RT‑q) PCR was used to measure the expression levels of miR‑502‑5p in GC tissues, normal adjacent tissues, a normal human gastric epithelial cell line (GES‑1) and two GC cell lines. miR‑502‑5p expression levels were significantly lower in GC tissues and GC cell lines compared with those in adjacent normal tissues and GES‑1 cells, respectively. Subsequently, the target genes of miR‑502‑5p were predicted, and it was demonstrated that the transcription factor SP1 was a direct target. SP1 expression, cell viability, migration and invasion, and SP1 protein levels were examined using RT‑qPCR, an MTT assay, Transwell assay and western blotting, respectively. Human GC cells were then transfected with an miR‑502‑5p mimic to emulate miR‑502‑5p overexpression, resulting in inhibition of the proliferation, migration and invasion capacities of human GC cells. Compared with the negative control, cells overexpressing miR‑502‑5p had decreased levels of SP1 mRNA and protein. These data suggest that miR‑502‑5p serves as a tumor suppressor gene by targeting SP1 to regulate the proliferation, migration and invasion of GC cells.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Peng X, Wu M, Liu W, Guo C, Zhan L and Zhan X: miR‑502‑5p inhibits the proliferation, migration and invasion of gastric cancer cells by targeting SP1. Oncol Lett 20: 2757-2762, 2020
APA
Peng, X., Wu, M., Liu, W., Guo, C., Zhan, L., & Zhan, X. (2020). miR‑502‑5p inhibits the proliferation, migration and invasion of gastric cancer cells by targeting SP1. Oncology Letters, 20, 2757-2762. https://doi.org/10.3892/ol.2020.11808
MLA
Peng, X., Wu, M., Liu, W., Guo, C., Zhan, L., Zhan, X."miR‑502‑5p inhibits the proliferation, migration and invasion of gastric cancer cells by targeting SP1". Oncology Letters 20.3 (2020): 2757-2762.
Chicago
Peng, X., Wu, M., Liu, W., Guo, C., Zhan, L., Zhan, X."miR‑502‑5p inhibits the proliferation, migration and invasion of gastric cancer cells by targeting SP1". Oncology Letters 20, no. 3 (2020): 2757-2762. https://doi.org/10.3892/ol.2020.11808