MicroRNA‑574‑3p inhibits the malignant behavior of liver cancer cells by targeting ADAM28

  • Authors:
    • Zhongming Zha
    • Fuxin Jia
    • Pingan Hu
    • Erhui Mai
    • Ting Lei
  • View Affiliations

  • Published online on: July 10, 2020     https://doi.org/10.3892/ol.2020.11852
  • Pages: 3015-3023
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Abstract

Liver cancer is one of the most common and aggressive tumors, and usually leads to a poor clinical outcome. Increasing evidence has demonstrated the important functions of microRNAs (miRs) in tumor progression. miR‑574‑3p has been reported as a tumor suppressor and potential therapeutic target in various types of cancer. However, the underlying mechanism of the effects of miR‑574‑3p in liver cancer remains unknown. In the present study, reverse transcription‑quantitative PCR was performed to detect miR‑574‑3p expression in liver cancer tissues, and the influence of miR‑574‑3p on cell growth was evaluated using the Cell Counting Kit‑8 assay, and cell migration and flow cytometry analyses. The present study revealed that miR‑574‑3p expression was downregulated in liver cancer tissues and cell lines. miR‑574‑3p overexpression, achieved by transfecting miR‑574‑3p mimics into liver cancer cells, reduced cell proliferation and migration, and promoted cell apoptosis. Mechanistically, ADAM metallopeptidase domain 28 (ADAM28) was identified as a miR‑574‑3p target via binding to the 3'‑untranslated region of the ADAM28 mRNA. Gain‑of‑function of miR‑574‑3p downregulated the expression levels of ADAM28 in liver cancer cells. Additionally, overexpression of ADAM28 significantly attenuated the suppressive effect of miR‑574‑3p on the growth of liver cancer cells. The present results provide novel insights into the function of the miR‑574‑3p/ADAM28 signaling pathway in liver cancer.

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September-2020
Volume 20 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zha Z, Jia F, Hu P, Mai E and Lei T: MicroRNA‑574‑3p inhibits the malignant behavior of liver cancer cells by targeting ADAM28. Oncol Lett 20: 3015-3023, 2020
APA
Zha, Z., Jia, F., Hu, P., Mai, E., & Lei, T. (2020). MicroRNA‑574‑3p inhibits the malignant behavior of liver cancer cells by targeting ADAM28. Oncology Letters, 20, 3015-3023. https://doi.org/10.3892/ol.2020.11852
MLA
Zha, Z., Jia, F., Hu, P., Mai, E., Lei, T."MicroRNA‑574‑3p inhibits the malignant behavior of liver cancer cells by targeting ADAM28". Oncology Letters 20.3 (2020): 3015-3023.
Chicago
Zha, Z., Jia, F., Hu, P., Mai, E., Lei, T."MicroRNA‑574‑3p inhibits the malignant behavior of liver cancer cells by targeting ADAM28". Oncology Letters 20, no. 3 (2020): 3015-3023. https://doi.org/10.3892/ol.2020.11852