MicroRNA 200b promotes mesenchymal‑to‑epithelial transition in anaplastic thyroid carcinoma

Tamagawa,Shunji; Enomoto,Keisuke; Gunduz,Esra; Gunduz,Mehmet; Sato,Fuyuki; Uchino,Shinya; Muragaki,Yasuteru; Hotomi,Muneki

doi:10.3892/ol.2020.11864

MicroRNA 200b promotes mesenchymal‑to‑epithelial transition in anaplastic thyroid carcinoma

Authors:
- Shunji Tamagawa
- Keisuke Enomoto
- Esra Gunduz
- Mehmet Gunduz
- Fuyuki Sato
- Shinya Uchino
- Yasuteru Muragaki
- Muneki Hotomi
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Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Wakayama Medical University, Wakayama, Wakayama 641‑8509, Japan, Department of Pathology, Wakayama Medical University, Wakayama, Wakayama 641‑8509, Japan, Noguchi Thyroid Clinic and Hospital Foundation, Beppu, Oita 874‑0902, Japan
Published online on: July 14, 2020 https://doi.org/10.3892/ol.2020.11864
Article Number: 3
Copyright: © Tamagawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Anaplastic thyroid cancer (ATC) remains a cancer with one of the worst prognoses, despite novel targeted therapies. The median survival rate has not improved for decades. Epithelial‑to‑mesenchymal transition (EMT) is a crucial step in physiological processes and in cancer progression, but the underlying mechanisms are not yet fully understood. The current study examined the role of microRNA (miR)‑200b in mesenchymal‑to‑epithelial transition in ATC. Total RNA and miR isolation were performed from ATC cell lines transfected with a miR‑200b mimic. After miR‑200b mimic transfection, expression levels of E‑cadherin, vimentin and zinc finger E‑box binding homeobox 1 (ZEB1) were confirmed by reverse transcription‑quantitative PCR and western blotting. Additionally, cell migration was evaluated using miR‑200b mimic and scrambled negative control‑transfected cells. A total of 14 human ATC and 15 non‑cancerous human thyroid tissues were immunohistochemically stained and scored as controls for E‑cadherin, vimentin and ZEB1. In ATC tissues and cell lines, the mesenchymal marker ZEB1 was significantly upregulated and the epithelial marker E‑cadherin was significantly downregulated. Additionally, the mesenchymal marker vimentin was significantly upregulated in ATC tissues and in one ATC cell line. MiR‑200b mimic transfection significantly increased vimentin and ZEB1 expression, but E‑cadherin expression remained below the measurement sensitivity. Furthermore, miR‑200b overexpression decreased cell migration. The current study suggested that miR‑200b may regulate the expression levels of mesenchymal markers such as vimentin and ZEB1 in ATC and may promote mesenchymal‑to‑epithelial transition.

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