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Article Open Access

STAT1 expression and HPV16 viral load predict cervical lesion progression

  • Authors:
    • Si Wu
    • Yingying Wu
    • Yiping Lu
    • Yuanyi Yue
    • Changwan Cui
    • Miao Yu
    • Shuang Wang
    • Miao Liu
    • Ying Zhao
    • Zhengrong Sun
  • View Affiliations / Copyright

    Affiliations: Department of Biobank, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Medical Examination Center, Shenyang Red Cross Hospital, Shenyang, Liaoning 110013, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 28
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    Published online on: July 17, 2020
       https://doi.org/10.3892/ol.2020.11889
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Abstract

Cervical cancer is the fourth leading cause of cancer‑associated mortality worldwide. However, its underlying molecular mechanisms are unclear. It is important to explore these mechanisms in order to identify novel diagnostic and prognostic biomarkers. The present study determined the association between STAT1 and human papillomavirus (HPV)16 in cervical lesions. STAT1 expression was detected by immunohistochemistry. Quantitative PCR was used to detect HPV16 viral load and STAT1 expression in cervical lesions. The potential associations among STAT1 expression, HPV16 viral load and the severity of cervical lesions in patients were analyzed using receiver operating characteristic (ROC) curves. The Cancer Genome Atlas database was used to analyze STAT1 expression and survival. High STAT1 expression was observed in 10.71 (3/28), 41.18 (14/34), 53.06 (26/49) and 90.00% (27/30) of normal tissue, low‑grade squamous intraepithelial lesion (LSIL), high‑grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma samples, respectively. The HPV16 copy number gradually increased with the progression of cervical lesions, with the highest copy number observed in cervical cancer samples. In addition, STAT1 expression was positively correlated with HPV16 viral load. Furthermore, ROC curve analysis demonstrated that the combination of STAT1 expression and HPV16 viral load was able to differentiate between LSIL/HSIL and cervical cancer samples. Bioinformatics analysis revealed that STAT1 expression was associated with improved survival in cervical cancer. Additionally, STAT1 expression was positively associated with the progression of cervical lesions, and HPV16 viral load may affect STAT1 expression. Overall, these findings indicate that STAT1 may be an indicator of the status of cervical lesions.
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Copy and paste a formatted citation
Spandidos Publications style
Wu S, Wu Y, Lu Y, Yue Y, Cui C, Yu M, Wang S, Liu M, Zhao Y, Sun Z, Sun Z, et al: STAT1 expression and HPV16 viral load predict cervical lesion progression. Oncol Lett 20: 28, 2020.
APA
Wu, S., Wu, Y., Lu, Y., Yue, Y., Cui, C., Yu, M. ... Sun, Z. (2020). STAT1 expression and HPV16 viral load predict cervical lesion progression. Oncology Letters, 20, 28. https://doi.org/10.3892/ol.2020.11889
MLA
Wu, S., Wu, Y., Lu, Y., Yue, Y., Cui, C., Yu, M., Wang, S., Liu, M., Zhao, Y., Sun, Z."STAT1 expression and HPV16 viral load predict cervical lesion progression". Oncology Letters 20.4 (2020): 28.
Chicago
Wu, S., Wu, Y., Lu, Y., Yue, Y., Cui, C., Yu, M., Wang, S., Liu, M., Zhao, Y., Sun, Z."STAT1 expression and HPV16 viral load predict cervical lesion progression". Oncology Letters 20, no. 4 (2020): 28. https://doi.org/10.3892/ol.2020.11889
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Spandidos Publications style
Wu S, Wu Y, Lu Y, Yue Y, Cui C, Yu M, Wang S, Liu M, Zhao Y, Sun Z, Sun Z, et al: STAT1 expression and HPV16 viral load predict cervical lesion progression. Oncol Lett 20: 28, 2020.
APA
Wu, S., Wu, Y., Lu, Y., Yue, Y., Cui, C., Yu, M. ... Sun, Z. (2020). STAT1 expression and HPV16 viral load predict cervical lesion progression. Oncology Letters, 20, 28. https://doi.org/10.3892/ol.2020.11889
MLA
Wu, S., Wu, Y., Lu, Y., Yue, Y., Cui, C., Yu, M., Wang, S., Liu, M., Zhao, Y., Sun, Z."STAT1 expression and HPV16 viral load predict cervical lesion progression". Oncology Letters 20.4 (2020): 28.
Chicago
Wu, S., Wu, Y., Lu, Y., Yue, Y., Cui, C., Yu, M., Wang, S., Liu, M., Zhao, Y., Sun, Z."STAT1 expression and HPV16 viral load predict cervical lesion progression". Oncology Letters 20, no. 4 (2020): 28. https://doi.org/10.3892/ol.2020.11889
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