Open Access

Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis

  • Authors:
    • Shougang Tan
    • Xiaoqun Hou
    • Lin Mei
  • View Affiliations

  • Published online on: August 13, 2020     https://doi.org/10.3892/ol.2020.11980
  • Article Number: 122
  • Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the effect of dihydrotanshinone I (DHI) on the survival of human glioma cells and the expression levels of ferroptosis‑associated proteins. Human U251 and U87 glioma cells were cultured in vitro and treated with different concentrations of DHI and/or the ferroptosis inhibitor ferrostatin‑1. A Cell Counting Kit‑8 assay was used to determine the cell survival rate. The cells were further analyzed to determine their 5‑, 12‑ and 15‑hydroxyeicosatetraenoic acid (HETE), lactate dehydrogenase (LDH) and malondialdehyde (MDA) levels, and reduced glutathione (GSH)/oxidized glutathione (GSSG) ratios. Western blotting was used to detect ferroptosis‑associated glutathione peroxidase 4 (GPX4) and long‑chain acyl‑CoA synthetase 4 (ACSL‑4). Changes in the mitochondrial membrane potential (MMP) were also observed using tetramethylrhodamine methyl ester staining and confocal fluorescence microscopy. The results revealed that DHI inhibited the proliferation of human glioma cells. Following treatment of the U251 and U87 cells with DHI, changes in the expression levels of ferroptosis‑associated proteins were observed; the expression level of GPX4 decreased and that of ACSL‑4 increased. DHI also increased the levels of LDH and MDA in the human glioma cells and reduced the GSH/GSSG ratio. The DHI‑treated cells also exhibited a marked reduction in MMP. Furthermore, ferrostatin‑1 blocked the DHI‑induced effects in human glioma cells. From these results, it may be concluded that DHI inhibits the proliferation of human glioma cells via the induction of ferroptosis.
View Figures
View References

Related Articles

Journal Cover

October-2020
Volume 20 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tan S, Hou X and Mei L: Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis. Oncol Lett 20: 122, 2020
APA
Tan, S., Hou, X., & Mei, L. (2020). Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis. Oncology Letters, 20, 122. https://doi.org/10.3892/ol.2020.11980
MLA
Tan, S., Hou, X., Mei, L."Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis". Oncology Letters 20.4 (2020): 122.
Chicago
Tan, S., Hou, X., Mei, L."Dihydrotanshinone I inhibits human glioma cell proliferation via the activation of ferroptosis". Oncology Letters 20, no. 4 (2020): 122. https://doi.org/10.3892/ol.2020.11980