Role of TG2 and TGF‑&beta;<sub>1</sub> in the pathogenesis of human breast cancer

Gao,Hai-Cheng; Huang,Yan-Zhi; Liu,Yu-Qi; Chen,Yan; Wang,Zhe-Ηui; Yin,Guang-Hao

doi:10.3892/ol.2020.12057

Role of TG2 and TGF‑β₁ in the pathogenesis of human breast cancer

Authors:
- Hai-Cheng Gao
- Yan-Zhi Huang
- Yu-Qi Liu
- Yan Chen
- Zhe-Ηui Wang
- Guang-Hao Yin
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Affiliations: Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Respiratory Medicine, Children's Hospital, Changchun, Jilin 130000, P.R. China, Department of Surgery, China‑Japan Union Hospital, Changchun, Jilin 130031, P.R. China, Department of Breast Surgery, Second Hospital, Jilin University, Changchun, Jilin 130041, P.R. China
Published online on: September 10, 2020 https://doi.org/10.3892/ol.2020.12057
Article Number: 221
Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study analyzed the role of transforming growth factor-β₁ (TGF‑β₁) and tissue transglutaminase (TG2) in breast cancer, as well as their protein levels in MCF‑7 cells treated with cisplatin. In addition, the present study investigated the effects of TG2 and TGF‑β₁ in MCF‑7 cells following TGF‑β₁ and TG2 inhibition or TGF‑β₁ induction. The protein levels of TG2 and TGF‑β₁ in breast cancer tissues and in MCF‑7 cells treated with cisplatin, TG2 and TGF‑β₁ inhibitors or 10 ng/ml TGF‑β₁ were analyzed by immunohistochemical staining, immunofluorescence and western blotting. The results revealed that the expression levels of TG2 and TGF‑β₁ in breast cancer tissues were significantly higher compared with those in paracancerous tissues. The fluorescence intensity of TG2 and TGF‑β₁ in MCF‑7 cells treated with cisplatin was lower compared with that in untreated MCF‑7 cells. Using bioinformatics analysis, the present study predicted that TGF‑β₁ may be associated with TG2. In addition, the expression levels of TGF‑β₁ and TG2 in MCF‑7 cells treated with inhibitors of TGF‑β₁ and TG2 were lower compared with those in untreated MCF‑7 cells. By contrast, the expression levels of TGF‑β₁ and TG2 in MCF‑7 cells treated with TGF‑β₁ were higher compared with those in untreated MCF‑7 cells. Therefore, the present study demonstrated that TGF‑β₁ and TG2 may serve an important role in breast cancer tissues and in MCF‑7 cells. In addition, it was revealed that TG2 and TGF‑β₁ may have a synergistic role in MCF‑7 cells.

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