Role of TG2 and TGF‑&beta;<sub>1</sub> in the pathogenesis of human breast cancer

Gao,Hai-Cheng; Huang,Yan-Zhi; Liu,Yu-Qi; Chen,Yan; Wang,Zhe-Ηui; Yin,Guang-Hao

doi:10.3892/ol.2020.12057

November-2020 Volume 20 Issue 5

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November-2020 Volume 20 Issue 5

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Article Open Access

Role of TG2 and TGF‑β₁ in the pathogenesis of human breast cancer

Authors:
- Hai-Cheng Gao
- Yan-Zhi Huang
- Yu-Qi Liu
- Yan Chen
- Zhe-Ηui Wang
- Guang-Hao Yin
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Affiliations: Department of Clinical Pharmacy, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Respiratory Medicine, Children's Hospital, Changchun, Jilin 130000, P.R. China, Department of Surgery, China‑Japan Union Hospital, Changchun, Jilin 130031, P.R. China, Department of Breast Surgery, Second Hospital, Jilin University, Changchun, Jilin 130041, P.R. China

Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 221
|
Published online on: September 10, 2020

https://doi.org/10.3892/ol.2020.12057
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Abstract

The present study analyzed the role of transforming growth factor-β₁ (TGF‑β₁) and tissue transglutaminase (TG2) in breast cancer, as well as their protein levels in MCF‑7 cells treated with cisplatin. In addition, the present study investigated the effects of TG2 and TGF‑β₁ in MCF‑7 cells following TGF‑β₁ and TG2 inhibition or TGF‑β₁ induction. The protein levels of TG2 and TGF‑β₁ in breast cancer tissues and in MCF‑7 cells treated with cisplatin, TG2 and TGF‑β₁ inhibitors or 10 ng/ml TGF‑β₁ were analyzed by immunohistochemical staining, immunofluorescence and western blotting. The results revealed that the expression levels of TG2 and TGF‑β₁ in breast cancer tissues were significantly higher compared with those in paracancerous tissues. The fluorescence intensity of TG2 and TGF‑β₁ in MCF‑7 cells treated with cisplatin was lower compared with that in untreated MCF‑7 cells. Using bioinformatics analysis, the present study predicted that TGF‑β₁ may be associated with TG2. In addition, the expression levels of TGF‑β₁ and TG2 in MCF‑7 cells treated with inhibitors of TGF‑β₁ and TG2 were lower compared with those in untreated MCF‑7 cells. By contrast, the expression levels of TGF‑β₁ and TG2 in MCF‑7 cells treated with TGF‑β₁ were higher compared with those in untreated MCF‑7 cells. Therefore, the present study demonstrated that TGF‑β₁ and TG2 may serve an important role in breast cancer tissues and in MCF‑7 cells. In addition, it was revealed that TG2 and TGF‑β₁ may have a synergistic role in MCF‑7 cells.

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1	Zhu W, Harvey S, Macura KJ, Euhus DM and Aremov D: Invasive breast cancer preferably and predominantly occurs at the interface between fibroglandular and adipose tissue. Clin Breast Cancer. 17:e11–e18. 2017. View Article : Google Scholar : PubMed/NCBI
2	Alipour S, Jannat F and Hosseini L: Teaching breast cancer screening via text messages as part of continuing education for working nurses: A case-control study. Asian Pac J Cancer Prev. 15:5607–5609. 2014. View Article : Google Scholar : PubMed/NCBI
3	Agnihotri N, Kumar S and Mehta K: Tissue transglutaminase as a central mediator in inflammation-induced progression of breast cancer. Breast Cancer Res. 15:2022013. View Article : Google Scholar : PubMed/NCBI
4	Wang J, Xi C, Yang X, Lu X, Yu K, Zhang Y and Gao R: LncRNA WT1-AS inhibits triple-negative breast cancer cell migration and invasion by downregulating transforming growth factor-β₁. Cancer Biother Radiopharm. 34:671–675. 2019. View Article : Google Scholar : PubMed/NCBI
5	Takai E, Tsukimoto M and Kojima S: TGF-β₁ downregulates COX-2 expression leading to decrease of PGE2 production in human lung cancer A549 cells, which is involved in fibrotic response to TGF-β₁. PLoS One. 8:e763462013. View Article : Google Scholar : PubMed/NCBI
6	Shao M, Cao L, Shen C, Satpathy M, Chelladurai B, Bigsby RM, Nakshatri H and Matei D: Epithelial-to-mesenchymal transition and ovarian tumor progression induced by tissue transglutaminase. Cancer Res. 69:9192–9120. 2009. View Article : Google Scholar : PubMed/NCBI
7	Humbert L, Ghozlan M, Canaff L, Tian J and Lebrun JJ: The leukemia inhibitory factor(LIF) and p21 mediate the TGFβ tumor suppressive effects in human cutaneous melanoma. BMC Cancer. 15:2002015. View Article : Google Scholar : PubMed/NCBI
8	Cao L, Shao M, Schilder J, Guise T, Mohammad KS and Matei D: Tissue transglutaminase links TGF-β, epithelial to mesenchymal transition and a stem cell phenotype in ovarian cancer. Oncogene. 31:2521–2534. 2012. View Article : Google Scholar : PubMed/NCBI
9	Efferth T, Konkimalla VB, Wang YF, Sauerbrey A, Meinhardt S, Zintl F, Mattern J and Volm M: Prediction of broad spectrum resistanc-e of tumors towards anticancer drugs. Clin Cancer Res. 14:2405–2412. 2008. View Article : Google Scholar : PubMed/NCBI
10	Segers-Nolten IM, Wilhelmus MM, Veldhuis G, van Rooijen BD, Drukarch B and Subramaniam V: Tissue transglutaminase modulates alpha-synuclein oligomeri-zation. Protein Sci. 17:1395–1402. 2008. View Article : Google Scholar : PubMed/NCBI
11	Schmid AW, Chiappe D, Pignat V, Grimminger V, Hang I, Moniatte M and Lashuel HA: Dissecting the mechanisms of tissue transglutaminase-induced cross-linkingof alpha-synuclein: Implications for the pathogenesis of parkinson disease. J BiolChem. 284:13128–13142. 2009.
12	Assi J, Srivastava G, Matta A, Chang MC, Walfish PG and Ralhan R: Transglutaminase 2 overexpression in tumor stroma identifies invasive ductal carcinomas of breast at high risk of recurrence. PLoS One. 8:e744372013. View Article : Google Scholar : PubMed/NCBI
13	Király R, Thangaraju K, Nagy Z, Collighan R, Nemes Z, Griffin M and Fésüs L: Isopeptidase activity of human transglutaminase 2: Disconnection from transamidation and characterization by kinetic parameters. Amino Acids. 48:31–40. 2016. View Article : Google Scholar : PubMed/NCBI
14	Zhao G, Zhang ZQ, Zhang B, Luo M, Sun YW and Wu ZY: Down-Regulation of TG2 expression by RNAi inhibits HSC proliferation and attenuates liver fibrosis. Int J Clin Exp Pathol. 4:513–520. 2011.PubMed/NCBI
15	Alves MJ, Figuerêdo RG, Azevedo FF, Cavallaro DA, Neto NI, Lima JD, Matos-Neto E, Radloff K, Riccardi DM, Camargo RG, et al: Adipose tissue fibrosis in human cancer cachexia: The role of TGF-β pathway. BMC Cancer. 17:1902017. View Article : Google Scholar : PubMed/NCBI
16	Zhang YP, Cao SY, Ma JY, Wang LQ and Wang BF: The change and significance of coagulation activity in bleomycin-induced lung fibrosis in rats. Zhonghua Jie He He Hu Xi Za Zhi. 28:541–544. 2005.(In Chinese). PubMed/NCBI
17	Vesprini D, Narod SA, Trachtenberg J, Crook J, Jalali F, Preiner J, Sridhar S and Bristow RG: The therapeutic ratio is preserved for radiotherapy or cisplatin treatment in BRCA2-mutated prostate cancers. Can Urol Assoc J. 5:E31–E35. 2011. View Article : Google Scholar : PubMed/NCBI

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