Open Access

Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer

  • Authors:
    • Xiao-Bo Cheng
    • Shuwen Wang
    • Hua Yang
    • Hanxing Tong
    • Guodong Shi
    • Lili Wu
    • Lei Shi
    • Hongwei Li
    • Songtao Ren
    • Yuhong Zhou
    • Norihiro Sato
    • Jian Zhou
  • View Affiliations

  • Published online on: September 4, 2020     https://doi.org/10.3892/ol.2020.12060
  • Article Number: 199
  • Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Receptor for hyaluronic acid (HA)‑mediated motility (RHAMM) expression is upregulated in pancreatic ductal adenocarcinoma (PDAC). In the present study, small interfering RNA knockdown was used to investigate the regulatory mechanism and function of RHAMM in PDAC cells. Reverse transcription‑quantitative PCR was used to measure the mRNA expression levels of RHAMM, hyaluronan synthases (HAS1, HAS2 and HAS3) and hyaluronidases (HYAL1, HYAL2 and HYAL3) in eight PDAC cell lines. Cell migration was assessed using a Transwell assay, while HA concentration was measured using an ELISA. The results revealed that RHAMM‑knockdown significantly increased migration in two PDAC cell lines, significantly decreased migration in one cell line and did not affect migration in the other cell lines, and was positively associated with changes in HA production. There was a linear negative correlation between RHAMM mRNA expression and HA concentration in PDAC cells and tissues. The negative correlation between RHAMM mRNA expression and HA concentration was demonstrated in other models, including SUIT2 cells treated with an HA inhibitor or stimulator and a system involving co‑culture of SUIT2 cells and stromal fibroblasts. The present findings demonstrated a negative correlation between RHAMM mRNA expression and HA production in a subset of PDAC cell lines. The efficacy of a therapeutic strategy targeting RHAMM should be carefully evaluated in future studies.
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November-2020
Volume 20 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cheng X, Wang S, Yang H, Tong H, Shi G, Wu L, Shi L, Li H, Ren S, Zhou Y, Zhou Y, et al: Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer. Oncol Lett 20: 199, 2020
APA
Cheng, X., Wang, S., Yang, H., Tong, H., Shi, G., Wu, L. ... Zhou, J. (2020). Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer. Oncology Letters, 20, 199. https://doi.org/10.3892/ol.2020.12060
MLA
Cheng, X., Wang, S., Yang, H., Tong, H., Shi, G., Wu, L., Shi, L., Li, H., Ren, S., Zhou, Y., Sato, N., Zhou, J."Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer". Oncology Letters 20.5 (2020): 199.
Chicago
Cheng, X., Wang, S., Yang, H., Tong, H., Shi, G., Wu, L., Shi, L., Li, H., Ren, S., Zhou, Y., Sato, N., Zhou, J."Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer". Oncology Letters 20, no. 5 (2020): 199. https://doi.org/10.3892/ol.2020.12060