Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer

Cheng,Xiao-Bo; Wang,Shuwen; Yang,Hua; Tong,Hanxing; Shi,Guodong; Wu,Lili; Zhou,Jian; Shi,Lei; Li,Hongwei; Ren,Songtao; Zhou,Yuhong; Sato,Norihiro

doi:10.3892/ol.2020.12060

November-2020 Volume 20 Issue 5

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November-2020 Volume 20 Issue 5

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Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer

Authors:
- Xiao-Bo Cheng
- Shuwen Wang
- Hua Yang
- Hanxing Tong
- Guodong Shi
- Lili Wu
- Jian Zhou
- Lei Shi
- Hongwei Li
- Songtao Ren
- Yuhong Zhou
- Norihiro Sato
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Affiliations: Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, P.R. China, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Department of Radiotherapy, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, P.R. China, Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China, Department of Surgery 1, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Fukuoka 807‑8555, Japan

Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 199
|
Published online on: September 4, 2020

https://doi.org/10.3892/ol.2020.12060
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Abstract

Receptor for hyaluronic acid (HA)‑mediated motility (RHAMM) expression is upregulated in pancreatic ductal adenocarcinoma (PDAC). In the present study, small interfering RNA knockdown was used to investigate the regulatory mechanism and function of RHAMM in PDAC cells. Reverse transcription‑quantitative PCR was used to measure the mRNA expression levels of RHAMM, hyaluronan synthases (HAS1, HAS2 and HAS3) and hyaluronidases (HYAL1, HYAL2 and HYAL3) in eight PDAC cell lines. Cell migration was assessed using a Transwell assay, while HA concentration was measured using an ELISA. The results revealed that RHAMM‑knockdown significantly increased migration in two PDAC cell lines, significantly decreased migration in one cell line and did not affect migration in the other cell lines, and was positively associated with changes in HA production. There was a linear negative correlation between RHAMM mRNA expression and HA concentration in PDAC cells and tissues. The negative correlation between RHAMM mRNA expression and HA concentration was demonstrated in other models, including SUIT2 cells treated with an HA inhibitor or stimulator and a system involving co‑culture of SUIT2 cells and stromal fibroblasts. The present findings demonstrated a negative correlation between RHAMM mRNA expression and HA production in a subset of PDAC cell lines. The efficacy of a therapeutic strategy targeting RHAMM should be carefully evaluated in future studies.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Negative regulation between the expression levels of receptor for hyaluronic acid‑mediated motility and hyaluronan leads to cell migration in pancreatic cancer

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