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Water‑soluble propolis and bee pollen of Trigona spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line

  • Authors:
    • Eri Amalia
    • Ajeng Diantini
    • Anas Subarnas
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java 45363, Indonesia
    Copyright: © Amalia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 274
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    Published online on: September 21, 2020
       https://doi.org/10.3892/ol.2020.12137
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Abstract

Bee products are best known as one of the beneficial natural products providing multiple pharmacological effects, such as antimicrobial, antiviral, anti‑inflammatory and anticancer effects. The present study aimed to identify potent products derived from the stingless bee Trigona spp. from Luwu Utara (South Sulawesi, Indonesia), focussing on the water‑soluble extract of propolis and bee pollen, against the proliferation of the human breast cancer MCF‑7 cell line. The results from DPPH (2,2‑diphenyl‑1‑picrylhydrazyl) method of antioxidant assay revealed that water‑soluble propolis and bee pollen had high antioxidant activity, with half‑maximal effective concentrations against DPPH radicals of 1.3 and 0.4 mg/ml, respectively. Additionally, water‑soluble propolis and bee pollen exhibited a significant antiproliferative activity in MCF‑7 cells, with IC50 values of 10.8±0.06 and 18.6±0.03 mg/ml, respectively (P<0.05). Significant cytotoxic effects were observed after 24 h of treatment via microscopic and flow cytometric analysis, where a morphological change toward late apoptosis was observed. By contrast, honey had low antioxidant activity and no antiproliferative effect in MCF‑7 cells. The water‑soluble propolis also exerted its antiproliferative effect in the human keratinocyte HaCaT cell line. The antiproliferative activity was similar (P>0.05) at 24 and 48 h of treatment, with IC50 at 2.7±0.06 mg/ml and <0.4 mg/ml, respectively. Notably, bee pollen was less toxic to HaCaT cells after 24 h of treatment than the water‑soluble propolis, with IC50>50 mg/ml. Its antiproliferative activity was significantly increased after 48 h of treatment, with IC50 at 9.6±0.07 mg/ml (P<0.05). In addition, similar to other poplar propolis, the high‑performance liquid chromatography‑ultraviolet and electrospray ionisation mass spectrometry analyses revealed that caffeic acid phenethyl ester was not the main bioactive compound of the samples examined. Furthermore, two major proteins (between ~50 and 75 kDa) were identified in the water‑soluble propolis and bee pollen. The present results suggested that water‑soluble propolis and bee pollen may have the potential to be elaborated further as a breast anticancer therapy.
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View References

1 

WHO, . Breast cancer WHO. 2019, https://www.who.int/cancer/prevention/diagnosis-screening/breast-cancer/en/Dec 15–2019

2 

Shah R, Rosso K and Nathanson SD: Pathogenesis, prevention, diagnosis and treatment of breast cancer. World J Clin Oncol. 5:283–298. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Amalia E, Diantini1 A and Subarnas A: Overview of current and future targets of breast cancer medicines. J Pharm Sci Res. 11:2385–2397. 2019.

4 

Siddiqui AA, Iram F, Siddiqui S and Sahu K: Role of natural products in drug discovery process. Int J Drug Dev Res. 6:172–204. 2016.

5 

Lichota A and Gwozdzinski K: Anticancer activity of natural compounds from plant and marine environment. Int J Mol Sci. 19:35332018. View Article : Google Scholar

6 

Choudhari MK, Haghniaz R, Rajwade JM and Paknikar KM: Anticancer activity of Indian stingless bee propolis: An in vitro study. Evid Based Complement Alternat Med. 2013:9282802013. View Article : Google Scholar : PubMed/NCBI

7 

Omene C, Kalac M, Wu J, Marchi E, Frenkel K and OConnor OA: Propolis and its active component, caffeic acid phenethyl ester (CAPE), modulate breast cancer therapeutic targets via an epigenetically mediated mechanism of action. J Cancer Sci Ther. 5:334–342. 2013.PubMed/NCBI

8 

Kustiawan PM, Puthong S, Arung ET and Chanchao C: In vitro cytotoxicity of Indonesian stingless bee products against human cancer cell lines. Asian Pac J Trop Biomed. 4:549–556. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Mărghitaş LA, Dezmirean D, Drâglă F and Bobiş O: Caffeic acid phenethyl ester (CAPE) in Romanian propolis. Bull UASVM Anim Sci Biotechnol. 71:111–114. 2014.

10 

Portokalakis I, Mohd Yusof HI, Ghanotakis DF, Nigam PS and Owusu-Apenten RK: Manuka honey-induced cytotoxicity against MCF7 breast cancer cells is correlated to total phenol content and antioxidant power. J Adv Biol Biotechnol. 8:1–10. 2016. View Article : Google Scholar

11 

Mahani, Nurhadi B, Subroto E and Herudiyanto M: Bee propolis Trigona spp. potential and uniqueness in Indonesia. Proc Univ Malaysia Teren Annu Sci. 2011:2011.

12 

Riswahyuli Y, Rohman A, Setyabudi FMCS and Raharjo S: Indonesian wild honey authenticity analysis using attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy combined with multivariate statistical techniques. Heliyon. 6:e036622020. View Article : Google Scholar : PubMed/NCBI

13 

Kitamura H: Effects of propolis extract and propolis-derived compounds on obesity and diabetes: Knowledge from cellular and animal models. Molecules. 24:43942019. View Article : Google Scholar

14 

Murtaza G, Karim S, Akram MR, Khan SA, Azhar S, Mumtaz A and Bin Asad MH: Caffeic acid phenethyl ester and therapeutic potentials. Biomed Res Int. 2014:1453422014. View Article : Google Scholar : PubMed/NCBI

15 

American Type Culture Collection: SOP: Thawing, Propagating and Cryopreserving of NCI-PBCF-HTB22 (MCF-7). Version 1.6. 2012.

16 

García-Viguera C, Ferreres F and Tomás-Barberán FA: Study of canadian propolis by GC-MS and HPLC. Z Natforsc C. 48c:731–735. 1993. View Article : Google Scholar

17 

Ji LI, Larregieu CA and Benet LZ: Classification of natural products as sources of drugs according to the biopharmaceutics drug disposition classification system (BDDCS). Chin J Nat Med. 14:888–897. 2016.PubMed/NCBI

18 

Tsume Y, Mudie DM, Langguth P, Amidon GE and Amidon GL: The biopharmaceutics classification system: Subclasses for in vivo predictive dissolution (IPD) methodology and IVIVC. Eur J Pharm Sci. 57:152–163. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Irnawati, Purba M, Mujadilah R and Sarmayani: Penetapan kadar vitamin C DAN UJI aktifitas antioksidan sari buah songi (Dillenia serrata Thunb.) terhadap radikal DPPH (Diphenylpicrylhydrazyl). Pharmacon. 6:40–44. 2017.(In Indonesian).

20 

Bankovaa VS, de Castro SL and Marcucci MC: Propolis: Recent advances in chemistry and plant origin. Apidologie. 31:3–15. 2000. View Article : Google Scholar

21 

Ahmed S and Othman NH: The anti-cancer effects of Tualang honey in modulating breast carcinogenesis: An experimental animal study. BMC Complement Altern Med. 17:2082017. View Article : Google Scholar : PubMed/NCBI

22 

Wlodkowic D, Skommer J and Darzynkiewicz Z: Flow cytometery-based apoptosis detection. Methods Mol Biol. 559:19–32. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Hingorani R, Deng J, Elia J, McIntyre C and Mittar D: Detection of apoptosis using the BD Annexin V FITC assay on the BD FACSVerse™ system. BD Bioscience application note. 2011:1–12. 2011.

24 

Comşa Ş, Cîmpean AM and Raica M: The story of MCF-7 breast cancer cell line: 40 tears of experience in research. Anticancer Res. 35:3147–3154. 2015.PubMed/NCBI

25 

Camarillo IG, Xiao F, Madhivanan S, Salameh T, Nichols M, Reece LM, Leary JF, Otto KJ, Natarajan A, Ramesh A and Sundararajan R: Low and high voltage electrochemotherapy for breast cancer: An in vitro model study. Electroporation Based Therapies Cancer. 55–102. 2014. View Article : Google Scholar

26 

Kiosses WB, Hahn KM, Giannelly G and Quaranta V: Characterization of morphological and cytoskeletal changes in MCF10A breast epithelial cells plated on Laminin-5: Comparison with breast cancer cell line MCF7. Cell Commun Adhes. 8:29–44. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Reed JC: Mechanisms of apoptosis. Am J Pathol. 157:1415–1430. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Niknafs B: Induction of apoptosis and non-apoptosis in human breast cancer cell line (MCF-7) by cisplatin and caffeine. Iran Biomed J. 15:130–133. 2011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Amalia E, Diantini A and Subarnas A: Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line. Oncol Lett 20: 274, 2020.
APA
Amalia, E., Diantini, A., & Subarnas, A. (2020). Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line. Oncology Letters, 20, 274. https://doi.org/10.3892/ol.2020.12137
MLA
Amalia, E., Diantini, A., Subarnas, A."Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line". Oncology Letters 20.5 (2020): 274.
Chicago
Amalia, E., Diantini, A., Subarnas, A."Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line". Oncology Letters 20, no. 5 (2020): 274. https://doi.org/10.3892/ol.2020.12137
Copy and paste a formatted citation
x
Spandidos Publications style
Amalia E, Diantini A and Subarnas A: Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line. Oncol Lett 20: 274, 2020.
APA
Amalia, E., Diantini, A., & Subarnas, A. (2020). Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line. Oncology Letters, 20, 274. https://doi.org/10.3892/ol.2020.12137
MLA
Amalia, E., Diantini, A., Subarnas, A."Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line". Oncology Letters 20.5 (2020): 274.
Chicago
Amalia, E., Diantini, A., Subarnas, A."Water‑soluble propolis and bee pollen of <em>Trigona</em> spp. from South Sulawesi Indonesia induce apoptosis in the human breast cancer MCF‑7 cell line". Oncology Letters 20, no. 5 (2020): 274. https://doi.org/10.3892/ol.2020.12137
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