Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2021 Volume 22 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Association of RGS20 expression with the progression and prognosis of renal cell carcinoma

  • Authors:
    • Lin Jiang
    • Jiangwei Shen
    • Ning Zhang
    • Yongchao He
    • Zhenghua Wan
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Caoxian People's Hospital, Heze, Shandong 274400, P.R. China, Department of Urology, The Fifth Hospital of Xiamen, Xiamen, Fujian 361101, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 643
    |
    Published online on: July 7, 2021
       https://doi.org/10.3892/ol.2021.12904
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Regulator of G protein signaling 20 (RGS20) has been shown to be highly expressed in various types of cancer. The present study aimed to investigate the effects of RGS20 in patients with renal cell carcinoma (RCC) and in RCC cells. Bioinformatics analysis was performed to analyze the role of RGS20 in RCC. Quantitative PCR and western blotting were used to determine the mRNA and protein expression levels of RGS20 in cells, respectively. After RGS20 inhibition, the proliferation, apoptosis, migration and invasiveness of A‑498 cells were tested using MTT assay, EdU assay, propidium iodide staining, Annexin V‑FITC/PI kit, wound healing assay and Transwell assay. High RGS20 expression was closely associated with the progression and immune infiltration of RCC, and may be considered as an independent indicator of poor prognosis in RCC. After knocking down RGS20, the proliferation, migration and invasiveness of cells were impaired, the cell cycle was arrested at the G0/G1 phase, and the level of apoptosis was increased. In addition, the mRNA expression levels of securin, CDC20 and cyclin B1 were decreased in RGS20‑knockdown cells. RGS20 expression was significantly associated with the infiltration level of activated CD4 T cells, type 1 T helper cells and activated dendritic cells. In summary, RGS20 expression was associated with RCC progression and poor prognosis; thus, it may be used to estimate the prognosis of RCC and may serve as a new potential treatment strategy for RCC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Khalil Ibrahim A: Trends of adult primary malignant renal tumors over 6 years. Pak J Med Sci. 29:1385–1388. 2013.PubMed/NCBI

2 

Zhai W, Zhu R, Ma J, Gong D, Zhang H, Zhang J, Chen Y, Huang Y, Zheng J and Xue W: A positive feed-forward loop between LncRNA-URRCC and EGFL7/P-AKT/FOXO3 signaling promotes proliferation and metastasis of clear cell renal cell carcinoma. Mol Cancer. 18:812019. View Article : Google Scholar : PubMed/NCBI

3 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Chen W, Sun K, Zheng R, Zeng H, Zhang S, Xia C, Yang Z, Li H, Zou X and He J: Cancer incidence and mortality in China, 2014. Chin J Cancer Res. 30:1–12. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Wong MCS, Goggins WB, Yip BHK, Fung FDH, Leung C, Fang Y, Wong SYS and Ng CF: Incidence and mortality of kidney cancer: Temporal patterns and global trends in 39 countries. Sci Rep. 7:156982017. View Article : Google Scholar : PubMed/NCBI

6 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2018. CA Cancer J Clin. 68:7–30. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Ravaud A, Motzer RJ, Pandha HS, George DJ, Pantuck AJ, Patel A, Chang YH, Escudier B, Donskov F, Magheli A, et al: Adjuvant sunitinib in high-risk renal-cell carcinoma after nephrectomy. N Engl J Med. 375:2246–2254. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Frank I, Blute ML, Cheville JC, Lohse CM, Weaver AL, Leibovich BC and Zincke H: A multifactorial postoperative surveillance model for patients with surgically treated clear cell renal cell carcinoma. J Urol. 170:2225–2232. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Patard JJ, Kim HL, Lam JS, Dorey FJ, Pantuck AJ, Zisman A, Ficarra V, Han KR, Cindolo L, De La Taille A, et al: Use of the University of California Los Angeles integrated staging system to predict survival in renal cell carcinoma: An international multicenter study. J Clin Oncol. 22:3316–3322. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Sorbellini M, Kattan MW, Snyder ME, Reuter V, Motzer R, Goetzl M, McKiernan J and Russo P: A postoperative prognostic nomogram predicting recurrence for patients with conventional clear cell renal cell carcinoma. J Urol. 173:48–51. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Calvo E, Schmidinger M, Heng DY, Grünwald V and Escudier B: Improvement in survival end points of patients with metastatic renal cell carcinoma through sequential targeted therapy. Cancer Treat Rev. 50:109–117. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Chen Z, Zhu R and Zheng J, Chen C, Huang C, Ma J, Xu C, Zhai W and Zheng J: Cryptotanshinone inhibits proliferation yet induces apoptosis by suppressing STAT3 signals in renal cell carcinoma. Oncotarget. 8:50023–50033. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Wang Y, Ho G, Zhang JJ, Nieuwenhuijsen B, Edris W, Chanda PK and Young KH: Regulator of G protein signaling Z1 (RGSZ1) interacts with Galpha i subunits and regulates Galpha i-mediated cell signaling. J Biol Chem. 277:48325–48332. 2002. View Article : Google Scholar : PubMed/NCBI

14 

De Vries L, Elenko E, Hubler L, Jones TL and Farquhar MG: GAIP is membrane-anchored by palmitoylation and interacts with the activated (GTP-bound) form of G alpha i subunits. Proc Natl Acad Sci USA. 93:15203–15208. 1996. View Article : Google Scholar : PubMed/NCBI

15 

Nunn C, Mao H, Chidiac P and Albert PR: RGS17/RGSZ2 and the RZ/A family of regulators of G-protein signaling. Semin Cell Dev Biol. 17:390–399. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Wang J, Ducret A, Tu Y, Kozasa T, Aebersold R and Ross EM: RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz gtpase-activating protein subfamily. J Biol Chem. 273:26014–26025. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Huang G, He X and Wei XL: lncRNA NEAT1 promotes cell proliferation and invasion by regulating miR-365/RGS20 in oral squamous cell carcinoma. Oncol Rep. 39:1948–1956. 2018.PubMed/NCBI

18 

Riker AI, Enkemann SA, Fodstad O, Liu S, Ren S, Morris C, Xi Y, Howell P, Metge B, Samant RS, et al: The gene expression profiles of primary and metastatic melanoma yields a transition point of tumor progression and metastasis. BMC Med Genomics. 1:132008. View Article : Google Scholar : PubMed/NCBI

19 

Li Q, Jin W, Cai Y, Yang F, Chen E, Ye D, Wang Q and Guan X: Regulator of G protein signaling 20 correlates with clinicopathological features and prognosis in triple-negative breast cancer. Biochem Biophys Res Commun. 485:693–697. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Li G, Wang M, Ren L, Li H, Liu Q, Ouyang Y, He L and Li F: Regulator of G protein signaling 20 promotes proliferation and migration in bladder cancer via NF-κB signaling. Biomed Pharmacother. 117:1091122019. View Article : Google Scholar : PubMed/NCBI

21 

Yang L, Lee MM, Leung MM and Wong YH: Regulator of G protein signaling 20 enhances cancer cell aggregation, migration, invasion and adhesion. Cell Signal. 28:1663–1672. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Li J, Hu L, Liu Y, Huang L, Mu Y, Cai X and Weng C: DDX19A senses viral RNA and mediates NLRP3-dependent inflammasome activation. J Immunol. 195:5732–5749. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Yao X, Hu W, Zhang J, Huang C, Zhao H and Yao X: Application of cAMP-dependent catalytic subunit β (PRKACB) low expression in predicting worse overall survival: A potential therapeutic target for colorectal carcinoma. J Cancer. 11:4841–4850. 2020. View Article : Google Scholar : PubMed/NCBI

25 

Subramanian A, Kuehn H, Gould J, Tamayo P and Mesirov JP: GSEA-P: A desktop application for gene set enrichment analysis. Bioinformatics. 23:3251–3253. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Chen H, Xu J, Hong J, Tang R, Zhang X and Fang JY: Long noncoding RNA profiles identify five distinct molecular subtypes of colorectal cancer with clinical relevance. Mol Oncol. 8:1393–1403. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Kapoor A, Yao W, Ying H, Hua S, Liewen A, Wang Q, Zhong Y, Wu CJ, Sadanandam A, Hu B, et al: Yap1 activation enables bypass of oncogenic kras addiction in pancreatic cancer. Cell. 179:12392019. View Article : Google Scholar : PubMed/NCBI

28 

Xu ZY, Zhao M, Chen W, Li K, Qin F, Xiang WW, Sun Y, Wei J, Yuan LQ, Li SK and Lin SH: Analysis of prognostic genes in the tumor microenvironment of lung adenocarcinoma. PeerJ. 8:e95302020. View Article : Google Scholar : PubMed/NCBI

29 

Yuan Q, Sun N, Zheng J, Wang Y, Yan X, Mai W, Liao Y and Chen X: Prognostic and immunological role of FUN14 domain containing 1 in pan-cancer: Friend or foe? Front Oncol. 9:15022020. View Article : Google Scholar : PubMed/NCBI

30 

Hurst JH and Hooks SB: Regulator of G-protein signaling (RGS) proteins in cancer biology. Biochem Pharmacol. 78:1289–1297. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Hurst JH, Mendpara N and Hooks SB: Regulator of G-protein signalling expression and function in ovarian cancer cell lines. Cell Mol Biol Lett. 14:153–174. 2009. View Article : Google Scholar : PubMed/NCBI

32 

James MA, Lu Y, Liu Y, Vikis HG and You M: RGS17, an overexpressed gene in human lung and prostate cancer, induces tumor cell proliferation through the cyclic AMP-PKA-CREB pathway. Cancer Res. 69:2108–2116. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Wiechec E, Overgaard J and Hansen LL: A fragile site within the HPC1 region at 1q25.3 affecting RGS16, RGSL1, and RGSL2 in human breast carcinomas. Genes Chromosomes Cancer. 47:766–780. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Fang G, Yu H and Kirschner MW: The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation. Genes Dev. 12:1871–1883. 1998. View Article : Google Scholar : PubMed/NCBI

35 

Brown NR, Lowe ED, Petri E, Skamnaki V, Antrobus R and Johnson LN: Cyclin B and cyclin A confer different substrate recognition properties on CDK2. Cell Cycle. 6:1350–1359. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Zou H, McGarry TJ, Bernal T and Kirschner MW: Identification of a vertebrate sister-chromatid separation inhibitor involved in transformation and tumorigenesis. Science. 285:418–422. 1999. View Article : Google Scholar : PubMed/NCBI

37 

Rajurkar M, Dang K, Fernandez-Barrena MG, Liu X, Fernandez-Zapico ME, Lewis BC and Mao J: IKBKE is required during KRAS-induced pancreatic tumorigenesis. Cancer Res. 77:320–329. 2017. View Article : Google Scholar : PubMed/NCBI

38 

Yang W, Qu Y, Tan B, Jia Y, Wang N, Hu P and Wang J: Prognostic significance of preoperative IKBKE expression in esophageal squamous cell carcinoma. Onco Targets Ther. 11:1305–1314. 2018. View Article : Google Scholar : PubMed/NCBI

39 

Wang X, Teng F, Lu J, Mu D, Zhang J and Yu J: Expression and prognostic role of IKBKE and TBK1 in stage I non-small cell lung cancer. Cancer Manag Res. 11:6593–6602. 2019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jiang L, Shen J, Zhang N, He Y and Wan Z: Association of RGS20 expression with the progression and prognosis of renal cell carcinoma. Oncol Lett 22: 643, 2021.
APA
Jiang, L., Shen, J., Zhang, N., He, Y., & Wan, Z. (2021). Association of RGS20 expression with the progression and prognosis of renal cell carcinoma. Oncology Letters, 22, 643. https://doi.org/10.3892/ol.2021.12904
MLA
Jiang, L., Shen, J., Zhang, N., He, Y., Wan, Z."Association of RGS20 expression with the progression and prognosis of renal cell carcinoma". Oncology Letters 22.3 (2021): 643.
Chicago
Jiang, L., Shen, J., Zhang, N., He, Y., Wan, Z."Association of RGS20 expression with the progression and prognosis of renal cell carcinoma". Oncology Letters 22, no. 3 (2021): 643. https://doi.org/10.3892/ol.2021.12904
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L, Shen J, Zhang N, He Y and Wan Z: Association of RGS20 expression with the progression and prognosis of renal cell carcinoma. Oncol Lett 22: 643, 2021.
APA
Jiang, L., Shen, J., Zhang, N., He, Y., & Wan, Z. (2021). Association of RGS20 expression with the progression and prognosis of renal cell carcinoma. Oncology Letters, 22, 643. https://doi.org/10.3892/ol.2021.12904
MLA
Jiang, L., Shen, J., Zhang, N., He, Y., Wan, Z."Association of RGS20 expression with the progression and prognosis of renal cell carcinoma". Oncology Letters 22.3 (2021): 643.
Chicago
Jiang, L., Shen, J., Zhang, N., He, Y., Wan, Z."Association of RGS20 expression with the progression and prognosis of renal cell carcinoma". Oncology Letters 22, no. 3 (2021): 643. https://doi.org/10.3892/ol.2021.12904
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team