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Article Open Access

MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1

Retraction in: /10.3892/ol.2024.14812
  • Authors:
    • Bin Kong
    • Shipeng Zhao
    • Xianwu Kang
    • Bo Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China
    Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 649
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    Published online on: July 9, 2021
       https://doi.org/10.3892/ol.2021.12910
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Abstract

Recently, miR‑133a‑3p has been identified as a marker for human colorectal cancer (CRC) and the association between miR‑133a‑3p and aquaporin 1 (AQP1) has been described in endothelial cells. However, the regulatory functions of the miR‑133a‑3p/AQP1 axis remain unclear in CRC. The present study analyzed the expression of miR‑133a‑3p and AQP1 in CRC tissues (n=56) and cell lines using reverse transcription‑quantitative PCR and western blot analysis. The χ2 test was used to assess the associations between miR‑133a‑3p/AQP1 and clinicopathological features of patients with CRC. Next, the functional role of miR‑133a‑3p/AQP1 in CRC was evaluated in vitro by performing Cell Counting Kit‑8 and Transwell assays. Moreover, the online software tool TargetScan7.1 was used to predict AQP1 as the target gene of miR‑133a‑3p, followed by validation using a luciferase reporter assay. The results showed that miR‑133a‑3p was significantly downregulated, while AQP1 was upregulated in CRC tissues and cell lines compared with corresponding controls. Clinically, it was demonstrated that miR‑133a‑3p/AQP1 expression was significantly associated with tumor TNM stage (P=0.020). Functional experiments indicated that miR‑133a‑3p‑overexpression remarkably suppressed, while knockdown promoted, cell proliferation, migration and invasion in CRC cells. Mechanically, AQP1 was identified and validated as a target gene of miR‑133a‑3p in CRC cells. The expression level of AQP1 mRNA was not correlated with miR‑133a‑3p expression in CRC tissues. Furthermore, AQP1‑knockdown induced, while overexpression reversed, the suppressive effects of miR‑133a‑3p on CRC cells. Taken together, these findings suggested that miR‑133a‑3p might be a tumor suppressor by suppressing cell proliferation, migration and invasion via targeting AQP1.
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Copy and paste a formatted citation
Spandidos Publications style
Kong B, Zhao S, Kang X and Wang B: MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812. Oncol Lett 22: 649, 2021.
APA
Kong, B., Zhao, S., Kang, X., & Wang, B. (2021). MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812. Oncology Letters, 22, 649. https://doi.org/10.3892/ol.2021.12910
MLA
Kong, B., Zhao, S., Kang, X., Wang, B."MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812". Oncology Letters 22.3 (2021): 649.
Chicago
Kong, B., Zhao, S., Kang, X., Wang, B."MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812". Oncology Letters 22, no. 3 (2021): 649. https://doi.org/10.3892/ol.2021.12910
Copy and paste a formatted citation
x
Spandidos Publications style
Kong B, Zhao S, Kang X and Wang B: MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812. Oncol Lett 22: 649, 2021.
APA
Kong, B., Zhao, S., Kang, X., & Wang, B. (2021). MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812. Oncology Letters, 22, 649. https://doi.org/10.3892/ol.2021.12910
MLA
Kong, B., Zhao, S., Kang, X., Wang, B."MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812". Oncology Letters 22.3 (2021): 649.
Chicago
Kong, B., Zhao, S., Kang, X., Wang, B."MicroRNA‑133a‑3p inhibits cell proliferation, migration and invasion in colorectal cancer by targeting AQP1 Retraction in /10.3892/ol.2024.14812". Oncology Letters 22, no. 3 (2021): 649. https://doi.org/10.3892/ol.2021.12910
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