Open Access

Association between the serum concentrations and mutational status of IL‑8, IL‑27 and VEGF and the expression levels of the hERG potassium channel gene in patients with colorectal cancer

  • Authors:
    • Marewan N. Abid
    • Fikry A. Qadir
    • Abbas Salihi
  • View Affiliations

  • Published online on: July 14, 2021     https://doi.org/10.3892/ol.2021.12926
  • Article Number: 665
  • Copyright: © Abid et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to determine the diagnostic value of the serum levels and mutational status of IL‑8, IL‑27 and VEGF, and the expression levels of human ether‑a‑go‑go‑related gene (hERG) in patients with colorectal cancer (CRC). The serum concentrations were determined using the ELISA technique and genotype variations of IL‑8, IL‑27 and VEGF were examined using Sanger sequencing, and the expression levels of hERG, which encodes a potassium channel, were determined by quantitative PCR, in blood and tissue samples obtained from 80 patients with CRC and 80 healthy individuals. The results of the present study revealed that the percentage of granulocytes and serum concentrations of carcinoembryonic antigen, IL‑8 and IL‑27 were significantly increased, whereas the percentage of lymphocytes was decreased in patients with CRC. In total, 31 mutations in three genes (eight mutations in VEGF, 13 mutations in IL‑27 and 10 mutations in IL‑8) were identified in patients with CRC. The relative mRNA expression levels of hERG were also significantly upregulated in tissue and blood samples of patients with CRC compared with those of healthy individuals. In conclusion, the results of the present study indicated that the increased concentrations and genetic variations of IL‑8, IL‑27 and VEGF may serve important roles in the development and angiogenic processes of CRC. These changes were concomitant with the upregulation of the expression levels of the potassium channel hERG.
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September-2021
Volume 22 Issue 3

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Spandidos Publications style
Abid MN, Qadir FA and Salihi A: Association between the serum concentrations and mutational status of IL‑8, IL‑27 and VEGF and the expression levels of the hERG potassium channel gene in patients with colorectal cancer. Oncol Lett 22: 665, 2021
APA
Abid, M.N., Qadir, F.A., & Salihi, A. (2021). Association between the serum concentrations and mutational status of IL‑8, IL‑27 and VEGF and the expression levels of the hERG potassium channel gene in patients with colorectal cancer. Oncology Letters, 22, 665. https://doi.org/10.3892/ol.2021.12926
MLA
Abid, M. N., Qadir, F. A., Salihi, A."Association between the serum concentrations and mutational status of IL‑8, IL‑27 and VEGF and the expression levels of the hERG potassium channel gene in patients with colorectal cancer". Oncology Letters 22.3 (2021): 665.
Chicago
Abid, M. N., Qadir, F. A., Salihi, A."Association between the serum concentrations and mutational status of IL‑8, IL‑27 and VEGF and the expression levels of the hERG potassium channel gene in patients with colorectal cancer". Oncology Letters 22, no. 3 (2021): 665. https://doi.org/10.3892/ol.2021.12926