Open Access

DNMT3B regulates proliferation of A549 cells through the microRNA‑152‑3p/NCAM1 pathway

  • Authors:
    • Bo Yang
    • Shiqing Huang
    • Hongming Chen
    • Rizhu Li
    • Shihao Hou
    • Jingjing Zhao
    • Yepeng Li
  • View Affiliations

  • Published online on: November 10, 2021     https://doi.org/10.3892/ol.2021.13129
  • Article Number: 11
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of the present study was to examine the epigenetic mechanism by which microRNA (miR)‑152‑3p regulates proliferation in non‑small cell lung cancer A549 cells via neural cell adhesion molecule 1 (NCAM1). Bisulfite sequencing PCR (BSP), the gold standard for methylation detection, uses bisulfite‑treated DNA to determine its pattern of methylation. Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5‑methylcytosine residues unaffected. It was conducted and demonstrated a relatively high level of methylation in the miR‑152‑3p promoter region. Chromatin immunoprecipitation was combined with PCR to detect the binding of DNA methyltransferase 3B (DNMT3B) protein to miR‑152‑3p, which tends to occur in the core region of the miR‑152‑3p gene in A549 cells. Luciferase assay identified NCAM1 as the target gene of miR‑152‑3p. MTT, colony formation and Transwell assays indicated that miR‑152‑3p could decrease cell proliferation and invasion and in addition to reducing the expression level of NCAM1. Overexpression of NCAM1 could attenuate the effect of miR‑152‑3p. DNMT3B knockdown decreased the proliferative ability of A549 cells and increased the expression of miR‑152‑3p, while decreased that of NCAM1. After treatment with miR‑152‑3p inhibitor, these effects were attenuated and the NCAM1 expression level was upregulated. The results indicated that miR‑152‑3p may suppress the proliferation of A549 cells by downregulating NCAM1. In addition, DNMT3B negatively regulated the expression of miR‑152‑3p via modulation of the methylation level in the miR‑152‑3p core region, thus mediating the proliferation of lung tumor cells.
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January-2022
Volume 23 Issue 1

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Copy and paste a formatted citation
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Spandidos Publications style
Yang B, Huang S, Chen H, Li R, Hou S, Zhao J and Li Y: DNMT3B regulates proliferation of A549 cells through the microRNA‑152‑3p/NCAM1 pathway. Oncol Lett 23: 11, 2022
APA
Yang, B., Huang, S., Chen, H., Li, R., Hou, S., Zhao, J., & Li, Y. (2022). DNMT3B regulates proliferation of A549 cells through the microRNA‑152‑3p/NCAM1 pathway. Oncology Letters, 23, 11. https://doi.org/10.3892/ol.2021.13129
MLA
Yang, B., Huang, S., Chen, H., Li, R., Hou, S., Zhao, J., Li, Y."DNMT3B regulates proliferation of A549 cells through the microRNA‑152‑3p/NCAM1 pathway". Oncology Letters 23.1 (2022): 11.
Chicago
Yang, B., Huang, S., Chen, H., Li, R., Hou, S., Zhao, J., Li, Y."DNMT3B regulates proliferation of A549 cells through the microRNA‑152‑3p/NCAM1 pathway". Oncology Letters 23, no. 1 (2022): 11. https://doi.org/10.3892/ol.2021.13129