MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1

Kong,Shuxin; Liu,Jianyang; Zhang,Bin; Lv,Feng; Yu,Yang; Qin,Tao

doi:10.3892/ol.2021.13133

MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1

Authors:
- Shuxin Kong
- Jianyang Liu
- Bin Zhang
- Feng Lv
- Yang Yu
- Tao Qin
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Affiliations: Department of Breast Surgery, The People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Department of Aortic Surgery, Fuwai Central China Cardiovascular Hospital, Heart Center of Henan Provincial People's Hospital, Zhengzhou, Henan 450001, P.R. China, Department of Hepatobiliary and Pancreatic Surgery, The People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China
Published online on: November 12, 2021 https://doi.org/10.3892/ol.2021.13133
Article Number: 15
Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs (miRNAs/miRs) function as key regulators in breast cancer (BC). The present study aimed to verify the function and molecular regulation of miR‑337‑3p in BC cells. Bioinformatics analysis was performed to screen key genes and miRNAs associated with BC. Reverse transcription‑quantitative PCR and western blot analyses were performed to detect RNA and protein expression levels. Cell Counting Kit‑8, BrdU and cell adhesion assays, and flow cytometric analysis were performed to assess the biological behaviors of BC cells. The dual‑luciferase reporter, RNA pull‑down assays, and Pearson's correlation analysis were performed to determine the association between miRNAs and mRNAs. Bioinformatics analysis revealed that miR‑337‑3p and cyclin‑dependent kinase 1 (CDK1) acted as key regulators in BC. In addition, miR‑337‑3p was expressed at low levels in BC cells and tissues, which suppressed BC progression. CDK1 expression was upregulated in BC cells and tissues, which was associated with increased cell proliferation and adhesion, as well as decreased apoptosis in BC. Notably, miR‑337‑3p targeted CDK1 to inhibit BC cell progression. Taken together, the results of the present study suggest that miR‑337‑3p plays a tumor‑suppressive role in BC by targeting CDK1.

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