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MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1

  • Authors:
    • Shuxin Kong
    • Jianyang Liu
    • Bin Zhang
    • Feng Lv
    • Yang Yu
    • Tao Qin
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Department of Aortic Surgery, Fuwai Central China Cardiovascular Hospital, Heart Center of Henan Provincial People's Hospital, Zhengzhou, Henan 450001, P.R. China, Department of Hepatobiliary and Pancreatic Surgery, The People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China
    Copyright: © Kong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 15
    |
    Published online on: November 12, 2021
       https://doi.org/10.3892/ol.2021.13133
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Abstract

MicroRNAs (miRNAs/miRs) function as key regulators in breast cancer (BC). The present study aimed to verify the function and molecular regulation of miR‑337‑3p in BC cells. Bioinformatics analysis was performed to screen key genes and miRNAs associated with BC. Reverse transcription‑quantitative PCR and western blot analyses were performed to detect RNA and protein expression levels. Cell Counting Kit‑8, BrdU and cell adhesion assays, and flow cytometric analysis were performed to assess the biological behaviors of BC cells. The dual‑luciferase reporter, RNA pull‑down assays, and Pearson's correlation analysis were performed to determine the association between miRNAs and mRNAs. Bioinformatics analysis revealed that miR‑337‑3p and cyclin‑dependent kinase 1 (CDK1) acted as key regulators in BC. In addition, miR‑337‑3p was expressed at low levels in BC cells and tissues, which suppressed BC progression. CDK1 expression was upregulated in BC cells and tissues, which was associated with increased cell proliferation and adhesion, as well as decreased apoptosis in BC. Notably, miR‑337‑3p targeted CDK1 to inhibit BC cell progression. Taken together, the results of the present study suggest that miR‑337‑3p plays a tumor‑suppressive role in BC by targeting CDK1.
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Copy and paste a formatted citation
Spandidos Publications style
Kong S, Liu J, Zhang B, Lv F, Yu Y and Qin T: MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1. Oncol Lett 23: 15, 2022.
APA
Kong, S., Liu, J., Zhang, B., Lv, F., Yu, Y., & Qin, T. (2022). MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1. Oncology Letters, 23, 15. https://doi.org/10.3892/ol.2021.13133
MLA
Kong, S., Liu, J., Zhang, B., Lv, F., Yu, Y., Qin, T."MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1". Oncology Letters 23.1 (2022): 15.
Chicago
Kong, S., Liu, J., Zhang, B., Lv, F., Yu, Y., Qin, T."MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1". Oncology Letters 23, no. 1 (2022): 15. https://doi.org/10.3892/ol.2021.13133
Copy and paste a formatted citation
x
Spandidos Publications style
Kong S, Liu J, Zhang B, Lv F, Yu Y and Qin T: MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1. Oncol Lett 23: 15, 2022.
APA
Kong, S., Liu, J., Zhang, B., Lv, F., Yu, Y., & Qin, T. (2022). MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1. Oncology Letters, 23, 15. https://doi.org/10.3892/ol.2021.13133
MLA
Kong, S., Liu, J., Zhang, B., Lv, F., Yu, Y., Qin, T."MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1". Oncology Letters 23.1 (2022): 15.
Chicago
Kong, S., Liu, J., Zhang, B., Lv, F., Yu, Y., Qin, T."MicroRNA‑337‑3p impedes breast cancer progression by targeting cyclin‑dependent kinase 1". Oncology Letters 23, no. 1 (2022): 15. https://doi.org/10.3892/ol.2021.13133
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