Long non‑coding RNA MIR17HG sponges microRNA‑21 to upregulate PTEN and regulate homoharringtonine‑based chemoresistance of acute myeloid leukemia cells

Yan,Jinhua; Yao,Ling; Li,Ping; Wu,Guohe; Lv,Xiaobin

doi:10.3892/ol.2021.13142

Long non‑coding RNA MIR17HG sponges microRNA‑21 to upregulate PTEN and regulate homoharringtonine‑based chemoresistance of acute myeloid leukemia cells

Authors:
- Jinhua Yan
- Ling Yao
- Ping Li
- Guohe Wu
- Xiaobin Lv
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Affiliations: Department of Hematology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China, Department of Gastroenterology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China, Jiangxi Key Laboratory of Cancer Metastasis and Precision Treatment, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China
Published online on: November 18, 2021 https://doi.org/10.3892/ol.2021.13142
Article Number: 24
Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding (lnc)RNA MIR17HG has been identified as a oncogene whose roles in acute myeloid leukemia (AML) remain unclear. The present study aimed to investigate the role of lncRNA MIR17HG in AML. Differential expression of MIR17HG in AML was determined by reverse transcription‑quantitative PCR. Overexpression assays and dual luciferase reporter assays were performed to determine the relationship between MIR17HG and microRNA (miR)‑21, and apoptosis was analyzed by using an apoptosis assay. The results showed that the expression of MIR17HG was decreased in AML, which was further decreased following homoharringtonine (HHT)‑based chemotherapy. Bioinformatics analysis predicted that miR‑21 could bind with MIR17HG. However, miR‑21 overexpression had no effect on the expression level of MIR17HG. Dual luciferase reporter assays were performed to verify the direct interaction between miR‑21 and MIR17HG. In addition, overexpression of MIR17HG and miR‑21 in AML cell lines up‑ and downregulated the expression level of PTEN, respectively. Furthermore, cell apoptosis showed that MIR17HG and PTEN overexpression enhanced cell apoptosis following cell treatment with HTT. However, miR‑21 overexpression exerted the opposite effect, since it reversed the effects of MIR17HG and PTEN overexpression in AML cell apoptosis. In conclusion, the current study suggested that MIR17HG could regulate the miR‑21/PTEN axis to modulate the chemoresistance of AML cells.

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