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Zinc‑finger protein 750 mitigates malignant biological behavior of oral CSC‑like cells enriched from parental CAL‑27 cells

  • Authors:
    • Cong Xu
    • Hong-Li Yang
    • Yi-Kun Yang
    • Li Pan
    • Hai-Ying Chen

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  • Published online on: November 19, 2021     https://doi.org/10.3892/ol.2021.13146
  • Article Number: 28
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Abstract

Oral squamous cell carcinoma (OSCC) is the most commonly occurring oral malignancy. Cancer stem cells (CSCs) are known to be responsible for cancer recurrence and metastasis. Zinc‑finger protein 750 (ZNF750) has been reported to inhibit OSCC cell proliferation and invasion. The present study aimed to elucidate the role of ZNF750 in the inhibition of the renewal ability of CSCs derived from the OSCC cell line, CAL‑27. The effects of ZNF750 on CSC‑like properties were examined using aldehyde dehydrogenase (ALDH), tumor sphere formation and colony formation assays. Reverse transcription‑quantitative PCR and western blotting were performed to detect the expression levels of octamer‑binding transcription factor 4, sex‑determining region Y‑box 2, the enhancer of zeste homolog 2 (Ezh2), embryonic ectoderm development (EED) and SUZ12 polycomb repressive complex 2 subunit (SUZ12), and for the identification of genes associated with metastasis. ZNF750 effectively attenuated CSC‑like cell self‑renewal abilities; ZNF750 decreased the ALDH‑positive cell population, tumor sphere and colony formation abilities, cell viability and stemness factors. Furthermore, the expression levels of Ezh2, EED and SUZ12 were decreased by ZNF750. ZNF750 inhibited MMP1, 3, 9 and 13 expression levels, and decreased the cell invasion and migratory abilities. Moreover, the expression of tissue inhibitors of matrix metalloproteinases‑1 was increased by ZNF750. However, opposite effects were observed following the knockdown of the ZNF750 gene. Overall, the present study demonstrated that ZNF750 has the potential to inhibit the renewal of CSC‑like cells enriched from parental CAL‑27 cells.
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January-2022
Volume 23 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • Source Normalized Impact per Paper (SNIP): 0.725
  • SCImago Journal Rank (SJR): 0.639
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Spandidos Publications style
Xu C, Yang H, Yang Y, Pan L and Chen H: Zinc‑finger protein 750 mitigates malignant biological behavior of oral CSC‑like cells enriched from parental CAL‑27 cells. Oncol Lett 23: 28, 2022
APA
Xu, C., Yang, H., Yang, Y., Pan, L., & Chen, H. (2022). Zinc‑finger protein 750 mitigates malignant biological behavior of oral CSC‑like cells enriched from parental CAL‑27 cells. Oncology Letters, 23, 28. https://doi.org/10.3892/ol.2021.13146
MLA
Xu, C., Yang, H., Yang, Y., Pan, L., Chen, H."Zinc‑finger protein 750 mitigates malignant biological behavior of oral CSC‑like cells enriched from parental CAL‑27 cells". Oncology Letters 23.1 (2022): 28.
Chicago
Xu, C., Yang, H., Yang, Y., Pan, L., Chen, H."Zinc‑finger protein 750 mitigates malignant biological behavior of oral CSC‑like cells enriched from parental CAL‑27 cells". Oncology Letters 23, no. 1 (2022): 28. https://doi.org/10.3892/ol.2021.13146