<em>Toxoplasma gondii</em> causes changes in the host's expression of cancer‑associated miRNAs

Wang,Lin; Wang,Lin; Wang,Lin; Wang,Lin; Wang,Ning; Wang,Ning; Wang,Ning; Wang,Ning; Zhao,Ying Hui; Zhao,Ying Hui; Zhao,Ying Hui; Zhao,Ying Hui; Lu,Gang; Lu,Gang; Lu,Gang; Lu,Gang

doi:10.3892/ol.2022.13267

Toxoplasma gondii causes changes in the host's expression of cancer‑associated miRNAs

Authors:
- Lin Wang
- Ning Wang
- Ying Hui Zhao
- Gang Lu
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Affiliations: Department of Epilepsy Center, Ji Nan Children's Hospital, Jinan, Shandong 250022, P.R. China, Department of Clinical Laboratory, Qingdao Third People's Hospital, Qingdao, Shandong 266041, P.R. China, Department of Pathogen Biology, School of Basic Medical Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250062, P.R. China
Published online on: March 15, 2022 https://doi.org/10.3892/ol.2022.13267
Article Number: 149
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Throughout the world, numerous individuals are infected with Toxoplasma gondii, which may improve immunity against cancer. Furthermore, microRNAs (miRs) may be differentially expressed in the host upon infection with T. gondii. In the present study, RNA‑sequencing analysis and reverse transcription‑quantitative PCR revealed that miR‑429‑3p, miR‑145a‑5p, miR‑211‑5p, miR‑31‑3p and miR‑135a‑5p were determined to be downregulated, while miR‑21a‑3p, miR‑135b‑5p, miR‑210‑5p and miR‑146‑3p were upregulated in mice post‑infection with T. gondii. Antitumor genes [TNF receptor superfamily member 11b, large tumor suppressor kinase (Lats)2 and Lats1] were identified as targets of miR‑429‑3p, miR‑145a‑5p, miR‑211‑5p, miR‑31‑3p and miR‑135a‑5p with a luciferase reporter assay. In addition, the protein levels of Lats2 and Lats1 were detected to be higher in T. gondii‑infected mice than in the control group. Therefore, these results provide favorable evidence for the suppression of cancer upon T. gondii infection and may give novel ideas for the treatment of tumors.

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