Circular RNA 0001666 inhibits colorectal cancer cell proliferation, invasion and stemness by inactivating the Wnt/β‑catenin <br />signaling pathway and targeting microRNA‑1229 Corrigendum in /10.3892/ol.2025.14885

Bai,Fei; Zuo,Chaohui; Ouyang,Yongzhong; Xiao,Ke; He,Zhuo; Yang,Zhi

doi:10.3892/ol.2022.13273

May-2022 Volume 23 Issue 5

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May-2022 Volume 23 Issue 5

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Article Open Access

Circular RNA 0001666 inhibits colorectal cancer cell proliferation, invasion and stemness by inactivating the Wnt/β‑catenin
signaling pathway and targeting microRNA‑1229

Corrigendum in: /10.3892/ol.2025.14885

Authors:
- Fei Bai
- Chaohui Zuo
- Yongzhong Ouyang
- Ke Xiao
- Zhuo He
- Zhi Yang
View Affiliations / Copyright

Affiliations: Department of Gastroduodenal and Pancreatic Surgery, Translational Medicine Research Center of Liver Cancer, Laboratory of Digestive Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, Hunan 410031, P.R. China, Department of Gastroduodenal and Pancreatic Surgery, Translational Medicine Research Center of Liver Cancer, Laboratory of Digestive Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, Hunan 410031, P.R. China, Department of Colorectal and Anal Surgery, Hepatobiliary and Enteric Surgery Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China

Copyright: © Bai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Article Number: 153
|
Published online on: March 16, 2022

https://doi.org/10.3892/ol.2022.13273
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Abstract

A previous bioinformatics study suggested that circular RNA 0001666 (circ_0001666) and its target microRNA (miR)‑1229 were associated with colorectal cancer (CRC) pathogenesis. However, the role of this interaction in the regulation of CRC cell malignancy remains unclear. Thus, the aim of the present study was to examine the interaction between circ_0001666 and miR‑1229, and its effects on CRC cell malignancy. circ_0001666 overexpression or knockdown plasmids were transfected into the HT‑29 and HCT‑116 cell lines. In addition, in rescue experiments, circ_000166 or miR‑1229 overexpression plasmids were transfected into the HT‑29 cell line, either alone or in combination. Following transfection, cell proliferation, apoptosis, invasion and the number of CD133⁺ cells were analyzed. The protein expression level of proteins in the Wnt/β‑catenin pathway was also examined. In both HT‑29 and HCT‑116 cell lines, circ_0001666 overexpression increased apoptosis, whilst inhibiting cell proliferation and invasion, and reducing the frequency of CD133⁺ cells. By contrast, circ_0001666 knockdown reduced apoptosis, but increased cell proliferation and the number of CD133⁺ cells. However, cell invasion remained unaffected. In addition, circ_0001666 expression levels negatively regulated those of miR‑1229, whereas miR‑1229 expression did not affect circ_0001666, in both the HT‑29 and HCT‑116 cell lines. Furthermore, a luciferase reporter assay confirmed that miR‑1229 directly bound to circ_0001666. In the HT‑29 cell line, miR‑1229 overexpression activated the Wnt/β‑catenin pathway, and promoted cell proliferation, invasion and stemness, while suppressing cell apoptosis. In addition, miR‑1229 overexpression reversed the effects of circ_0001666 overexpression. In conclusion, circ_0001666 suppresses CRC cell proliferation, invasion and stemness by inhibiting the Wnt/β‑catenin signaling pathway by targeting miR‑1229, and may represent a potential target for CRC treatment.

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