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3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma

  • Authors:
    • Jonas Heid
    • Annette Affolter
    • Yvonne Jakob
    • Johann Kern
    • Nicole Rotter
    • Esther Tenschert
    • Anne Lammert
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology Head and Neck Surgery, University Medical Centre Mannheim, D‑68167 Mannheim, Germany
    Copyright: © Heid et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 177
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    Published online on: April 14, 2022
       https://doi.org/10.3892/ol.2022.13297
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Abstract

Epidermal growth factor receptor (EGFR) upregulation is a typical characteristic of head and neck squamous cell carcinoma (HNSCC). However, tyrosine kinase inhibitors have not yet been able to achieve enough therapeutic benefit in clinical trials to justify their use in standard therapy regimens. At present, little is known about the reasons for this treatment failure. In the present study, the HNSCC cell lines UM‑SCC‑11B and UM‑SCC‑22B were tested for their response to tyrosine kinase inhibitors (TKI) under 2D and 3D cell culture conditions. Absorption and luciferase‑based viability assays were used for this, as well as optical evaluation via fluorescence microscopy. In addition, EGFR and HER3 expression as well as the downstream signalling pathways PI3K/AKT/mTOR and RAS/RAF/MEK/ERK were investigated using western blotting. Cell line UM‑SCC‑11B revealed a strong resistance to lapatinib under 3D cell culture conditions, while a good response to TKI therapy was observed under 2D cell culture conditions. An associated overexpression of phosphorylated HER3 under 3D cell culture conditions offered a plausible explanation for the altered treatment response. The results of the present study represent an idea of how signalling mechanisms of cancer cells can be changed using different cell culture methods. Overall, 3D cell culture could be an important component in the analysis of resistance mechanisms in cancer therapy.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Sturgis EM, Wei Q and Spitz MR: Descriptive epidemiology and risk factors for head and neck cancer. Semin Oncol. 31:726–733. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Blot WJ, McLaughlin JK, Winn DM, Austin DF, Greenberg RS, Preston-Martin S, Bernstein L, Schoenberg JB, Stemhagen A and Fraumeni JF Jr: Smoking and drinking in relation to oral and pharyngeal cancer. Cancer Res. 48:3282–3287. 1988.PubMed/NCBI

4 

Grandis JR and Tweardy DJ: Elevated levels of transforming growth factor alpha and epidermal growth factor receptor messenger RNA are early markers of carcinogenesis in head and neck cancer. Cancer Res. 53:3579–3584. 1993.PubMed/NCBI

5 

Scaltriti M and Baselga J: The epidermal growth factor receptor pathway: A model for targeted therapy. Clin Cancer Res. 12:5268–5272. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Iqbal N and Iqbal N: Imatinib: A breakthrough of targeted therapy in cancer. Chemother Res Pract. 2014:3570272014.PubMed/NCBI

7 

Harvey RD, Adams VR, Beardslee T and Medina P: Afatinib for the treatment of EGFR mutation-positive NSCLC: A review of clinical findings. J Oncol Pharm Pract. 26:1461–1474. 2020. View Article : Google Scholar : PubMed/NCBI

8 

Xu ZQ, Zhang Y, Li N, Liu PJ, Gao L, Gao X and Tie XJ: Efficacy and safety of lapatinib and trastuzumab for HER2-positive breast cancer: A systematic review and meta-analysis of randomised controlled trials. BMJ Open. 7:e0130532017. View Article : Google Scholar : PubMed/NCBI

9 

Vermorken JB, Trigo J, Hitt R, Koralewski P, Diaz-Rubio E, Rolland F, Knecht R, Amellal N, Schueler A and Baselga J: Open-label, uncontrolled, multicenter phase II study to evaluate the efficacy and toxicity of cetuximab as a single agent in patients with recurrent and/or metastatic squamous cell carcinoma of the head and neck who failed to respond to platinum-based therapy. J Clin Oncol. 25:2171–2177. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Ferrarotto R and Gold KA: Afatinib in the treatment of head and neck squamous cell carcinoma. Expert Opin Investig Drugs. 23:135–143. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Cohen RB: Current challenges and clinical investigations of epidermal growth factor receptor (EGFR)- and ErbB family-targeted agents in the treatment of head and neck squamous cell carcinoma (HNSCC). Cancer Treat Rev. 40:567–577. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Kozakiewicz P and Grzybowska-Szatkowska L: Application of molecular targeted therapies in the treatment of head and neck squamous cell carcinoma. Oncol Lett. 15:7497–7505. 2018.PubMed/NCBI

13 

Weiss JM, Bagley S, Hwang WT, Bauml J, Olson JG, Cohen RB, Hayes DN and Langer C: Capecitabine and lapatinib for the first-line treatment of metastatic/recurrent head and neck squamous cell carcinoma. Cancer. 122:2350–2355. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Agarwal V, Subash A, Nayar RC and Rao V: Is EGFR really a therapeutic target in head and neck cancers? J Surg Oncol. 119:685–686. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Cohen EE, Licitra LF, Burtness B, Fayette J, Gauler T, Clement PM, Grau JJ, Del Campo JM, Mailliez A, Haddad RI, et al: Biomarkers predict enhanced clinical outcomes with afatinib versus methotrexate in patients with second-line recurrent and/or metastatic head and neck cancer. Ann Oncol. 28:2526–2532. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Picon H and Guddati AK: Mechanisms of resistance in head and neck cancer. Am J Cancer Res. 10:2742–2751. 2020.PubMed/NCBI

17 

Shuford S, Lipinski L, Abad A, Smith AM, Rayner M, O'Donnell L, Stuart J, Mechtler LL, Fabiano AJ, Edenfield J, et al: Prospective prediction of clinical drug response in high-grade gliomas using an ex vivo 3D cell culture assay. Neurooncol Adv. 3:vdab0652021.PubMed/NCBI

18 

Costa EC, Moreira AF, de Melo-Diogo D, Gaspar VM, Carvalho MP and Correia IJ: 3D tumor spheroids: An overview on the tools and techniques used for their analysis. Biotechnol Adv. 34:1427–1441. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Prince ME, Sivanandan R, Kaczorowski A, Wolf GT, Kaplan MJ, Dalerba P, Weissman IL, Clarke MF and Ailles LE: Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma. Proc Natl Acad Sci USA. 104:973–978. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Clay MR, Tabor M, Owen JH, Carey TE, Bradford CR, Wolf GT, Wicha MS and Prince ME: Single-marker identification of head and neck squamous cell carcinoma cancer stem cells with aldehyde dehydrogenase. Head Neck. 32:1195–1201. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Tahmasebi E, Alikhani M, Yazdanian A, Yazdanian M, Tebyanian H and Seifalian A: The current markers of cancer stem cell in oral cancers. Life Sci. 249:1174832020. View Article : Google Scholar : PubMed/NCBI

22 

Yamada KM and Cukierman E: Modeling tissue morphogenesis and cancer in 3D. Cell. 130:601–610. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Ayuso JM, Vitek R, Swick AD, Skala MC, Wisinski KB, Kimple RJ, Lambert PF and Beebe DJ: Effects of culture method on response to EGFR therapy in head and neck squamous cell carcinoma cells. Sci Rep. 9:124802019. View Article : Google Scholar : PubMed/NCBI

24 

Chan YH, Lee YC, Hung CY, Yang PJ, Lai PC and Feng SW: Three-dimensional spheroid culture enhances multipotent differentiation and stemness capacities of human dental pulp-derived mesenchymal stem cells by modulating MAPK and NF-kB signaling pathways. Stem Cell Rev Rep. 17:1810–1826. 2021. View Article : Google Scholar : PubMed/NCBI

25 

Lee HK, Noh MH, Hong SW, Kim SM, Kim SH, Kim YS, Broaddus VC and Hur DY: Erlotinib activates different cell death pathways in EGFR-mutant lung cancer cells grown in 3D versus 2D culture systems. Anticancer Res. 41:1261–1269. 2021. View Article : Google Scholar : PubMed/NCBI

26 

DelNero P, Lane M, Verbridge SS, Kwee B, Kermani P, Hempstead B, Stroock A and Fischbach C: 3D culture broadly regulates tumor cell hypoxia response and angiogenesis via pro-inflammatory pathways. Biomaterials. 55:110–118. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Fontoura JC, Viezzer C, Dos Santos FG, Ligabue RA, Weinlich R, Puga RD, Antonow D, Severino P and Bonorino C: Comparison of 2D and 3D cell culture models for cell growth, gene expression and drug resistance. Mater Sci Eng C Mater Biol Appl. 107:1102642020. View Article : Google Scholar : PubMed/NCBI

28 

Schmidt M, Scholz CJ, Polednik C and Roller J: Spheroid-based 3-dimensional culture models: Gene expression and functionality in head and neck cancer. Oncol Rep. 35:2431–2440. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Imamura Y, Mukohara T, Shimono Y, Funakoshi Y, Chayahara N, Toyoda M, Kiyota N, Takao S, Kono S, Nakatsura T and Minami H: Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer. Oncol Rep. 33:1837–1843. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Mosaad E, Chambers K, Futrega K, Clements J and Doran MR: Using high throughput microtissue culture to study the difference in prostate cancer cell behavior and drug response in 2D and 3D co-cultures. BMC Cancer. 18:5922018. View Article : Google Scholar : PubMed/NCBI

31 

Watanabe S, Yonesaka K, Tanizaki J, Nonagase Y, Takegawa N, Haratani K, Kawakami H, Hayashi H, Takeda M, Tsurutani J and Nakagawa K: Targeting of the HER2/HER3 signaling axis overcomes ligand-mediated resistance to trastuzumab in HER2-positive breast cancer. Cancer Med. 8:1258–1268. 2019. View Article : Google Scholar : PubMed/NCBI

32 

Temraz S, Mukherji D and Shamseddine A: Dual targeting of HER3 and EGFR in colorectal tumors might overcome anti-EGFR resistance. Crit Rev Oncol Hematol. 101:151–157. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Wang D, Qian G, Zhang H, Magliocca KR, Nannapaneni S, Amin AR, Rossi M, Patel M, El-Deiry M, Wadsworth JT, et al: HER3 targeting sensitizes HNSCC to cetuximab by reducing HER3 activity and HER2/HER3 dimerization: Evidence from cell line and patient-derived xenograft models. Clin Cancer Res. 23:677–686. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Iida M, Bahrar H, Brand TM, Pearson HE, Coan JP, Orbuch RA, Flanigan BG, Swick AD, Prabakaran PJ, Lantto J, et al: Targeting the HER family with Pan-HER effectively overcomes resistance to cetuximab. Mol Cancer Ther. 15:2175–2186. 2016. View Article : Google Scholar : PubMed/NCBI

35 

von der Grün J, Rödel F, Brandts C, Fokas E, Guckenberger M, Rödel C and Balermpas P: Targeted therapies and immune-checkpoint inhibition in head and neck squamous cell carcinoma: Where do we stand today and where to go? Cancers (Basel). 11:4722019. View Article : Google Scholar : PubMed/NCBI

36 

Guo Y, Ahn MJ, Chan A, Wang CH, Kang JH, Kim SB, Bello M, Arora RS, Zhang Q, He X, et al: Afatinib versus methotrexate as second-line treatment in Asian patients with recurrent or metastatic squamous cell carcinoma of the head and neck progressing on or after platinum-based therapy (LUX-head & neck 3): an open-label, randomised phase III trial. Ann Oncol. 30:1831–1839. 2019. View Article : Google Scholar : PubMed/NCBI

37 

Phan TG and Croucher PI: The dormant cancer cell life cycle. Nat Rev Cancer. 20:398–411. 2020. View Article : Google Scholar : PubMed/NCBI

38 

Faber A, Aderhold C, Goessler UR, Hoermann K, Schultz JD, Umbreit C, Walliczek U and Stern-Straeter J: Interaction of a CD44+ head and neck squamous cell carcinoma cell line with a stromal cell-derived factor-1-expressing supportive niche: An in vitro model. Oncol Lett. 7:82–86. 2014. View Article : Google Scholar : PubMed/NCBI

39 

Lammert A, Affolter A, Gvaramia D, Heid J, Jungbauer F, Scherl C, Tenschert E, Rotter N, Willett N and Kern J: The tumor stem cell niche of head and neck-point of intersection with therapeutic potential? Laryngorhinootologie. 100:23–29. 2021.(In German). PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Heid J, Affolter A, Jakob Y, Kern J, Rotter N, Tenschert E and Lammert A: 3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma. Oncol Lett 23: 177, 2022.
APA
Heid, J., Affolter, A., Jakob, Y., Kern, J., Rotter, N., Tenschert, E., & Lammert, A. (2022). 3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma. Oncology Letters, 23, 177. https://doi.org/10.3892/ol.2022.13297
MLA
Heid, J., Affolter, A., Jakob, Y., Kern, J., Rotter, N., Tenschert, E., Lammert, A."3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma". Oncology Letters 23.6 (2022): 177.
Chicago
Heid, J., Affolter, A., Jakob, Y., Kern, J., Rotter, N., Tenschert, E., Lammert, A."3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma". Oncology Letters 23, no. 6 (2022): 177. https://doi.org/10.3892/ol.2022.13297
Copy and paste a formatted citation
x
Spandidos Publications style
Heid J, Affolter A, Jakob Y, Kern J, Rotter N, Tenschert E and Lammert A: 3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma. Oncol Lett 23: 177, 2022.
APA
Heid, J., Affolter, A., Jakob, Y., Kern, J., Rotter, N., Tenschert, E., & Lammert, A. (2022). 3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma. Oncology Letters, 23, 177. https://doi.org/10.3892/ol.2022.13297
MLA
Heid, J., Affolter, A., Jakob, Y., Kern, J., Rotter, N., Tenschert, E., Lammert, A."3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma". Oncology Letters 23.6 (2022): 177.
Chicago
Heid, J., Affolter, A., Jakob, Y., Kern, J., Rotter, N., Tenschert, E., Lammert, A."3D cell culture alters signal transduction and drug response in head and neck squamous cell carcinoma". Oncology Letters 23, no. 6 (2022): 177. https://doi.org/10.3892/ol.2022.13297
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