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Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5

  • Authors:
    • Jiaxing Wei
    • Yanmeizhi Wu
    • Xiaona Zhang
    • Jingxue Sun
    • Jian Li
    • Jingjing Li
    • Xu Yang
    • Hong Qiao
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 206
    |
    Published online on: May 12, 2022
       https://doi.org/10.3892/ol.2022.13327
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Abstract

Type 2 diabetes mellitus (T2DM) has an increased risk of cancer. In the present study, the relationship between T2DM and 13 types of cancer was analyzed and key methylation genes were searched. First, DNA methylation and mRNA expression were obtained data for T2DM and 13 types of cancer from The Cancer Genome Atlas and Gene Expression Omnibus. The t‑test was used to screen the differentially methylated expression overlapping genes (DE‑MGs) in T2DM and cancer on both methylation and expression levels. DE‑MGs are weighted based on the methylation and projected into the human protein interaction network. The correlation between T2DM and each type of cancer was analyzed, and key genes were identified. The results showed that 293 DE‑MGs were related to T2DM and 3307 were related to cancer. The network found that T2DM is more related to colorectal cancer (CRC) compare with the other 12 types of cancer. A total of 5 from 8 candidate genes were associated with CRC. A total of 28 clinical patients were used to validate these 5 genes. A CRC tissue sample was collected from each patient, as well as a paracancerous sample that served as a control. A total of 56 tissue samples were divided into 4 groups: control group, T2DM group, CRC group and T2DM with CRC group (combination group). Compared with the control group, the methylation level of adenylate cyclase 5 (ADCY5), neuregulin 1 and ELAV‑like RNA‑binding protein 4 in the combination group was significantly upregulated, and the mRNA level was significantly downregulated. Furthermore, based on the methylation level of ADCY5, the correlation coefficient between the combination group and the T2DM group was greater than that of the CRC group. In conclusion, T2DM is most likely to be associated with CRC among 13 common types of cancer based on methylation characteristics. An upregulated methylation of ADCY5 in T2DM may have a higher risk of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Wei J, Wu Y, Zhang X, Sun J, Li J, Li J, Yang X and Qiao H: Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5. Oncol Lett 24: 206, 2022.
APA
Wei, J., Wu, Y., Zhang, X., Sun, J., Li, J., Li, J. ... Qiao, H. (2022). Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5. Oncology Letters, 24, 206. https://doi.org/10.3892/ol.2022.13327
MLA
Wei, J., Wu, Y., Zhang, X., Sun, J., Li, J., Li, J., Yang, X., Qiao, H."Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5". Oncology Letters 24.1 (2022): 206.
Chicago
Wei, J., Wu, Y., Zhang, X., Sun, J., Li, J., Li, J., Yang, X., Qiao, H."Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5". Oncology Letters 24, no. 1 (2022): 206. https://doi.org/10.3892/ol.2022.13327
Copy and paste a formatted citation
x
Spandidos Publications style
Wei J, Wu Y, Zhang X, Sun J, Li J, Li J, Yang X and Qiao H: Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5. Oncol Lett 24: 206, 2022.
APA
Wei, J., Wu, Y., Zhang, X., Sun, J., Li, J., Li, J. ... Qiao, H. (2022). Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5. Oncology Letters, 24, 206. https://doi.org/10.3892/ol.2022.13327
MLA
Wei, J., Wu, Y., Zhang, X., Sun, J., Li, J., Li, J., Yang, X., Qiao, H."Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5". Oncology Letters 24.1 (2022): 206.
Chicago
Wei, J., Wu, Y., Zhang, X., Sun, J., Li, J., Li, J., Yang, X., Qiao, H."Type 2 diabetes is more closely associated with risk of colorectal cancer based on elevated DNA methylation levels of ADCY5". Oncology Letters 24, no. 1 (2022): 206. https://doi.org/10.3892/ol.2022.13327
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