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Article Open Access

Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension

  • Authors:
    • Stéphane Chabaud
    • Ève Pellerin
    • Christophe Caneparo
    • Cassandra Ringuette‑Goulet
    • Frédéric Pouliot
    • Stéphane Bolduc
  • View Affiliations / Copyright

    Affiliations: Centre de Recherche en Organogénèse Expérimentale (Experimental Organogenesis Research Center)/LOEX, Regenerative Medicine Division, CHU de Québec‑Laval University Research Center, Enfant‑Jésus Hospital, Quebec, QC G1J 1Z4, Canada, Department of Surgery, Faculty of Medicine, Laval University, Quebec, QC G1V 4G2, Canada
    Copyright: © Chabaud et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 220
    |
    Published online on: May 20, 2022
       https://doi.org/10.3892/ol.2022.13341
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Abstract

During the process of tumor growth, cancer cells will be subjected to intermittent hypoxia. This results from the delay in the development of the vascular network in relation to the proliferation of cancer cells. The hypoxic nature of a tumor has been demonstrated as a negative factor for patient survival. To evaluate the impact of hypoxia on the survival and migration properties of low and high‑grade bladder cancer cell lines, two low‑grade (MGHU‑3 and SW‑780) and two high‑grade (SW‑1710 and T24) bladder cancer cell lines were cultured in normoxic (20% O2) or hypoxic atmospheric conditions (2% O2). The response of bladder cancer cell lines to hypoxic atmospheric cell culture conditions was examined under several parameters, including epithelial‑mesenchymal transition, doubling time and metabolic activities, thrombospondin‑1 expression, whole Matrix Metallo‑Proteinase activity, migration and resistance to oxidative stress. The low‑grade cell line response to hypoxia was heterogeneous even if it tended to adopt a more aggressive profile. Hypoxia enhanced migration and pro‑survival properties of MGHU‑3 cells, whereas these features were reduced for the SW‑780 cell line cultured under low oxygen tension. The responses of tested high‑grade cell lines were more homogeneous and tended to adopt a less aggressive profile. Hypoxia drastically changed some of the bladder cancer cell line properties, for example matrix metalloproteinases expression for all cancer cells but also switch in glycolytic metabolism of low grade cancer cells. Overall, studying bladder cancer cells in hypoxic environments are relevant for the translation from in vitro findings to in vivo context.
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Copy and paste a formatted citation
Spandidos Publications style
Chabaud S, Pellerin È, Caneparo C, Ringuette‑Goulet C, Pouliot F and Bolduc S: Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension. Oncol Lett 24: 220, 2022.
APA
Chabaud, S., Pellerin, È., Caneparo, C., Ringuette‑Goulet, C., Pouliot, F., & Bolduc, S. (2022). Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension. Oncology Letters, 24, 220. https://doi.org/10.3892/ol.2022.13341
MLA
Chabaud, S., Pellerin, È., Caneparo, C., Ringuette‑Goulet, C., Pouliot, F., Bolduc, S."Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension". Oncology Letters 24.1 (2022): 220.
Chicago
Chabaud, S., Pellerin, È., Caneparo, C., Ringuette‑Goulet, C., Pouliot, F., Bolduc, S."Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension". Oncology Letters 24, no. 1 (2022): 220. https://doi.org/10.3892/ol.2022.13341
Copy and paste a formatted citation
x
Spandidos Publications style
Chabaud S, Pellerin È, Caneparo C, Ringuette‑Goulet C, Pouliot F and Bolduc S: Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension. Oncol Lett 24: 220, 2022.
APA
Chabaud, S., Pellerin, È., Caneparo, C., Ringuette‑Goulet, C., Pouliot, F., & Bolduc, S. (2022). Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension. Oncology Letters, 24, 220. https://doi.org/10.3892/ol.2022.13341
MLA
Chabaud, S., Pellerin, È., Caneparo, C., Ringuette‑Goulet, C., Pouliot, F., Bolduc, S."Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension". Oncology Letters 24.1 (2022): 220.
Chicago
Chabaud, S., Pellerin, È., Caneparo, C., Ringuette‑Goulet, C., Pouliot, F., Bolduc, S."Bladder cancer cell lines adapt their aggressiveness profile to oxygen tension". Oncology Letters 24, no. 1 (2022): 220. https://doi.org/10.3892/ol.2022.13341
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