Open Access

Cancer stem cell marker expression and methylation status in patients with colorectal cancer

  • Authors:
    • Sandra Mersakova
    • Katarina Janikova
    • Michal Kalman
    • Juraj Marcinek
    • Marian Grendar
    • Martin Vojtko
    • Roman Kycina
    • Miroslav Pindura
    • Jan Janik
    • Peter Mikolajcik
    • Eva Gabonova
    • Ludovit Laca
    • Ester Mejstrikova
    • Erika Halasova
    • Jan Strnadel
    • Zora Lasabova
  • View Affiliations

  • Published online on: May 27, 2022     https://doi.org/10.3892/ol.2022.13352
  • Article Number: 231
  • Copyright: © Mersakova et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The number of individuals diagnosed with colorectal cancer (CRC) has been on an alarming upward trajectory over the past decade. In some countries, this cancer represents one of the most frequently diagnosed types of neoplasia. Therefore, it is an important demand to study the pathology underlying this disease to gain insights into the mechanism of resistance to treatment. Resistance of tumors to chemotherapy and tumor aggressiveness have been associated with a minor population of neoplastic cells, which are considered to be responsible for tumor recurrence. These types of neoplastic cells are known as cancer stem cells, which have been previously reported to serve an important role in pathogenesis of this malignant disease. Slovakia has one of the highest incidence rates of CRC worldwide. In the present study, the aim was to classify the abundance of selected stem cell markers (CD133, CD166 and Lgr5) in CRC tumors using flow cytometry. In addition, the methylation status of selected genomic regions of CRC biomarkers (ADAMTS16, MGMT, PROM1 (CD133), LGR5 and ALCAM) was investigated by pyrosequencing in a cohort of patients from Martin University Hospital, Martin, Slovakia. Samples from both primary tumors and metastatic tumors were tested. Analysis of DNA methylation in the genomic regions of indicated five CRC biomarkers was also performed, which revealed the highest levels of methylation in the A disintegrin and metalloproteinase with thrombospondin motifs 16 and O6‑methyguanine‑DNA methyl transferase genes, whereas the lowest levels of methylation were found in genes expressing prominin‑1, leucine‑rich repeat‑containing G‑protein‑coupled receptor 5 and activated leukocyte cell adhesion molecule. Furthermore, tumor tissues from metastases showed significantly higher levels of CD133+ cells compared with that in primary tumors. Higher levels of CD133+ cells correlated with TNM stage and the invasiveness of CRC into the lymphatic system. Although relatively small number of samples was processed, CD133 marker was consider to be important marker in pathology of CRC.
View Figures
View References

Related Articles

Journal Cover

July-2022
Volume 24 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Mersakova S, Janikova K, Kalman M, Marcinek J, Grendar M, Vojtko M, Kycina R, Pindura M, Janik J, Mikolajcik P, Mikolajcik P, et al: Cancer stem cell marker expression and methylation status in patients with colorectal cancer. Oncol Lett 24: 231, 2022.
APA
Mersakova, S., Janikova, K., Kalman, M., Marcinek, J., Grendar, M., Vojtko, M. ... Lasabova, Z. (2022). Cancer stem cell marker expression and methylation status in patients with colorectal cancer. Oncology Letters, 24, 231. https://doi.org/10.3892/ol.2022.13352
MLA
Mersakova, S., Janikova, K., Kalman, M., Marcinek, J., Grendar, M., Vojtko, M., Kycina, R., Pindura, M., Janik, J., Mikolajcik, P., Gabonova, E., Laca, L., Mejstrikova, E., Halasova, E., Strnadel, J., Lasabova, Z."Cancer stem cell marker expression and methylation status in patients with colorectal cancer". Oncology Letters 24.1 (2022): 231.
Chicago
Mersakova, S., Janikova, K., Kalman, M., Marcinek, J., Grendar, M., Vojtko, M., Kycina, R., Pindura, M., Janik, J., Mikolajcik, P., Gabonova, E., Laca, L., Mejstrikova, E., Halasova, E., Strnadel, J., Lasabova, Z."Cancer stem cell marker expression and methylation status in patients with colorectal cancer". Oncology Letters 24, no. 1 (2022): 231. https://doi.org/10.3892/ol.2022.13352