Open Access

FABP7 inhibits proliferation and invasion abilities of cutaneous squamous cell carcinoma cells via the Notch signaling pathway

  • Authors:
    • Zhonghui Sun
    • Yunyi Guo
    • Danlu Zhang
    • Guolong Zhang
    • Ying Zhang
    • Xiuli Wang
  • View Affiliations

  • Published online on: June 10, 2022     https://doi.org/10.3892/ol.2022.13374
  • Article Number: 254
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cutaneous squamous cell carcinoma (CSCC) is one of the most common non‑melanoma skin cancers worldwide. Fatty acid‑binding protein 7 (FABP7) has been reported to be involved in the occurrence, development, metastasis and prognosis of various tumors. In addition, downregulated FABP7 expression was demonstrated in cutaneous malignant melanoma in a previous study. Therefore, we speculated that FABP7 may be a biomarker for CSCC diagnosis. The aim of the present study was to determine the molecular mechanism underlying the effects of FABP7 in CSCC, which may provide a new diagnostic biomarker or treatment target for CSCC. Reverse transcription‑PCR, western blotting and immunohistochemistry assays were performed to detect the expression levels of FABP7 in CSCC tissues and cells. Overexpression of FABP7 was achieved in A431 and colo‑16 cell lines by transfection with an overexpression vector (oeFABP7). Cell proliferation, colony formation, migration and invasion were detected by Cell Counting Kit‑8, crystal violet, scratch and Transwell assays, respectively. Following FABP7 overexpression, western blotting was used to determine the expression levels of proliferation‑, invasion‑ and Notch pathway‑associated proteins, including Snail, N‑cadherin, Twist, matrix metalloproteinase (MMP)‑2, MMP‑7, Notch 1 and Notch 3. In addition a CSCC model in nude mice was constructed. Immunohistochemistry was used to determine the expression levels of FABP7, Ki67, Notch 1 and Notch 3. It was demonstrated that FABP7 expression levels were significantly reduced in human CSCC tissues and cells compared with normal samples. Overexpression of FABP7 inhibited the proliferation, invasion and migration abilities of A431 and colo‑16 cells compared with those in the negative control group. In addition, transfection with oeFABP7 reduced the expression levels of proliferation‑, invasion‑ and Notch pathway‑associated proteins compared with those in the negative control group. Overexpression of FABP7 also reduced the growth of CSCC tumors in vivo and inhibited the expression of Ki67, Notch 1 and Notch 3. Therefore, the results of the present study suggested that FABP7 may inhibit the proliferation and invasion of CSCC cells via the Notch signaling pathway.
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August-2022
Volume 24 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Sun Z, Guo Y, Zhang D, Zhang G, Zhang Y and Wang X: FABP7 inhibits proliferation and invasion abilities of cutaneous squamous cell carcinoma cells via the Notch signaling pathway. Oncol Lett 24: 254, 2022
APA
Sun, Z., Guo, Y., Zhang, D., Zhang, G., Zhang, Y., & Wang, X. (2022). FABP7 inhibits proliferation and invasion abilities of cutaneous squamous cell carcinoma cells via the Notch signaling pathway. Oncology Letters, 24, 254. https://doi.org/10.3892/ol.2022.13374
MLA
Sun, Z., Guo, Y., Zhang, D., Zhang, G., Zhang, Y., Wang, X."FABP7 inhibits proliferation and invasion abilities of cutaneous squamous cell carcinoma cells via the Notch signaling pathway". Oncology Letters 24.2 (2022): 254.
Chicago
Sun, Z., Guo, Y., Zhang, D., Zhang, G., Zhang, Y., Wang, X."FABP7 inhibits proliferation and invasion abilities of cutaneous squamous cell carcinoma cells via the Notch signaling pathway". Oncology Letters 24, no. 2 (2022): 254. https://doi.org/10.3892/ol.2022.13374