Organic extract of Geodia cydonium induces cell cycle block in human mesothelioma cells

Di Meo,Francesco; Esposito,Roberta; Cuciniello,Rossana; Favale,Gregorio; Arenga,Mario; Ruocco,Nadia; Nuzzo,Genoveffa; Fontana,Angelo; Filosa,Stefania; Crispi,Stefania; Costantini,Maria

doi:10.3892/ol.2022.13406

Organic extract of Geodia cydonium induces cell cycle block in human mesothelioma cells

Authors:
- Francesco Di Meo
- Roberta Esposito
- Rossana Cuciniello
- Gregorio Favale
- Mario Arenga
- Nadia Ruocco
- Genoveffa Nuzzo
- Angelo Fontana
- Stefania Filosa
- Stefania Crispi
- Maria Costantini
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Affiliations: Department of Biology, Agriculture and Food Science, Institute of Biosciences and BioResources‑UOS Naples, National Research Council, I‑80131 Naples, Italy, Department of Ecosustainable Marine Biotechnology, Zoological Station Anton Dohrn, I‑80121 Naples, Italy, Department of Biology, Agriculture and Food Science, Institute of Biosciences and BioResources‑UOS Naples, National Research Council, I‑80131 Naples, Italy, Department of Chemical Sciences and Materials Technologies, Institute of Biomolecular Chemistry, National Research Council, I‑80078 Naples, Italy
Published online on: June 28, 2022 https://doi.org/10.3892/ol.2022.13406
Article Number: 286
Copyright: © Di Meo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The serious side effects caused by chemotherapeutics and the development of cancer chemoresistance represent the most significant limitations in the treatment of cancer. Some alternative approaches have been developed in recent years, which are based on natural compounds, and have allowed important advances in cancer therapeutics. During the last 50 years, sponges have been considered a promising source of natural products from the marine environment, representing ~30% of all marine natural products. Among sponges, the Mediterranean species Geodia cydonium represents a potential source of these type of products with considerable biotechnological interest as pharmaceutical agents. The present study demonstrated the antiproliferative effect of an organic G. cydonium extract (GEOCYDO) against three human mesothelioma cell lines, MSTO‑211H (MSTO), NCI‑H2452 (NCI) and Ist‑Mes2 (Mes2), which differ in their sensitivity (MSTO and NCI) and resistance (Mes2) to standard combined treatment with cisplatin and piroxicam. To this aim, the activity of the extract was evaluated by analyzing its effects on cell viability, cancer properties and cell cycle progression by means of colony formation assay, cell cycle analysis and protein expression analysis. The results revealed, in mesothelioma, this extract was able to reduce self‑renewal, cell migration and it could induce cell cycle arrest in G₀/G₁ stage, thus blocking cell proliferation. In conclusion, to the best of our knowledge, the present results indicated for the first time that GEOCYDO can contain active compounds able to affect cell proliferation in mesothelioma, suggesting that it could be considered as a potential novel drug source for cancer treatment.

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