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Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells

  • Authors:
    • Linhong Liao
    • Hui Cheng
    • Shusong Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Ganzhou Maternal and Child Health Hospital, Ganzhou, Jiangxi 341000, P.R. China, Department of Emergency, Ganzhou People's Hospital, Ganzhou, Jiangxi 341000, P.R. China, Department of Oncology, Xi'an International Medical Center Hospital, Xi'an, Shaanxi 710100, P.R. China
    Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 317
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    Published online on: July 19, 2022
       https://doi.org/10.3892/ol.2022.13438
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Abstract

Abstract. Breast cancer is one of the most frequently diagnosed types of cancer worldwide. The present study aimed to investigate the role and underlying regulatory mechanism of non‑structural maintenance of chromosome condensin I complex subunit H (NCAPH) in the malignant progression and cisplatin (DDP) resistance of breast cancer cells. Therefore, the mRNA and protein expression levels of NCAPH were first determined in breast cancer cells via reverse transcription‑quantitative PCR and western blotting. Furthermore, following transfection of NCAPH interference plasmids, the effect of NCAPH knockdown on cell proliferation, migration, invasion were also assessed using CCK‑8, wound healing and Transwell assays. Apoptosis was evaluated using TUNEL assay, and western blotting was performed in breast cancer cells and DDP‑resistant breast cancer cells. The association between NCAPH and its downstream target, aurora kinase B (AURKB), was verified using bioinformatic analysis and the co‑immunoprecipitation assay. Furthermore, the effect of AURKB overexpression on the aforementioned processes and the Akt/mTOR signaling pathway were also assessed. The results demonstrated that NCAPH mRNA and protein expression levels were significantly upregulated in breast cancer cells, whereas NCAPH knockdown significantly attenuated the proliferation, migration and invasion of breast cancer cells. NCAPH silencing also exacerbated the apoptosis of DDP‑resistant breast cancer cells. AURKB mRNA and protein expression levels were also significantly upregulated in MCF‑7 cells, whereas its overexpression significantly reversed the effects of NCAPH knockdown on breast cancer cells and the Akt/mTOR signaling pathway. Overall, NCAPH knockdown significantly downregulated AURKB mRNA and protein expression levels to block the Akt/mTOR signaling pathway and inhibited breast cancer cell proliferation, migration, invasion, and aggravate DDP‑resistant breast cancer cell apoptosis, indicating that NCAPH may serve as a promising therapeutic target for breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liao L, Cheng H and Liu S: Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells. Oncol Lett 24: 317, 2022.
APA
Liao, L., Cheng, H., & Liu, S. (2022). Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells. Oncology Letters, 24, 317. https://doi.org/10.3892/ol.2022.13438
MLA
Liao, L., Cheng, H., Liu, S."Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells". Oncology Letters 24.3 (2022): 317.
Chicago
Liao, L., Cheng, H., Liu, S."Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells". Oncology Letters 24, no. 3 (2022): 317. https://doi.org/10.3892/ol.2022.13438
Copy and paste a formatted citation
x
Spandidos Publications style
Liao L, Cheng H and Liu S: Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells. Oncol Lett 24: 317, 2022.
APA
Liao, L., Cheng, H., & Liu, S. (2022). Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells. Oncology Letters, 24, 317. https://doi.org/10.3892/ol.2022.13438
MLA
Liao, L., Cheng, H., Liu, S."Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells". Oncology Letters 24.3 (2022): 317.
Chicago
Liao, L., Cheng, H., Liu, S."Non‑SMC condensin I complex subunit H promotes the malignant progression and cisplatin resistance of breast cancer MCF‑7 cells". Oncology Letters 24, no. 3 (2022): 317. https://doi.org/10.3892/ol.2022.13438
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