Open Access

Knockdown of lncRNA JPX suppresses IL‑1β‑stimulated injury in chondrocytes through modulating an miR‑25‑3p/PPID axis

  • Authors:
    • Zhiyong Ren
    • Liguo Tang
    • Zhonghua Ding
    • Jun Song
    • Hailiang Zheng
    • Dongzhu Li
  • View Affiliations

  • Published online on: September 19, 2022     https://doi.org/10.3892/ol.2022.13508
  • Article Number: 388
  • Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of this study was to investigate the potential mechanisms of long noncoding (lnc) RNA Just proximal to X‑inactive specific transcript (JPX) in interleukin (IL)‑1β‑stimulated chondrocytes. Human C28/I2 chondrocytes were treated with IL‑1β to simulate osteoarthritic (OA) injury. The expression levels of JPX, microRNA (miRNA/miR)‑25‑3p, and peptidylprolyl isomerase D (PPID) were measured using reverse transcription‑quantitative PCR or western blotting. The IL‑1β‑stimulated injury was assessed using a Cell Counting Kit‑8 assay, flow cytometry, and western blot analysis. The targeted relationship between miR‑25‑3p, JPX, and PPID was verified using a dual‑luciferase reporter and RNA immunoprecipitation (RIP) assays. The results showed that JPX expression was upregulated in OA patients and IL‑1β‑stimulated chondrocytes. JPX knockdown enhanced cell viability and suppressed apoptosis of IL‑1β‑stimulated chondrocytes. miR‑25‑3p inhibition rescued the inhibitory effect of JPX knockdown on IL‑1β‑stimulated injury. PPID overexpression eliminated the effects of JPX knockdown on IL‑1β‑stimulated chondrocytes. In conclusion, JPX knockdown increased cell viability and reduced apoptosis in IL‑1β‑stimulated chondrocytes, and this involved modulation of a miR‑25‑3p/PPID axis.
View Figures
View References

Related Articles

Journal Cover

November-2022
Volume 24 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ren Z, Tang L, Ding Z, Song J, Zheng H and Li D: Knockdown of lncRNA JPX suppresses IL‑1β‑stimulated injury in chondrocytes through modulating an miR‑25‑3p/PPID axis. Oncol Lett 24: 388, 2022
APA
Ren, Z., Tang, L., Ding, Z., Song, J., Zheng, H., & Li, D. (2022). Knockdown of lncRNA JPX suppresses IL‑1β‑stimulated injury in chondrocytes through modulating an miR‑25‑3p/PPID axis. Oncology Letters, 24, 388. https://doi.org/10.3892/ol.2022.13508
MLA
Ren, Z., Tang, L., Ding, Z., Song, J., Zheng, H., Li, D."Knockdown of lncRNA JPX suppresses IL‑1β‑stimulated injury in chondrocytes through modulating an miR‑25‑3p/PPID axis". Oncology Letters 24.5 (2022): 388.
Chicago
Ren, Z., Tang, L., Ding, Z., Song, J., Zheng, H., Li, D."Knockdown of lncRNA JPX suppresses IL‑1β‑stimulated injury in chondrocytes through modulating an miR‑25‑3p/PPID axis". Oncology Letters 24, no. 5 (2022): 388. https://doi.org/10.3892/ol.2022.13508