Open Access

Analysis of the chemotactic factors for tumor‑infiltrating fibrocytes and their prognostic significances in lung cancer

  • Authors:
    • Makoto Tobiume
    • Atsushi Mitsuhashi
    • Atsuro Saijo
    • Hirokazu Ogino
    • Tania Afroj
    • Hirohisa Ogawa
    • Hisatsugu Goto
    • Seidai Sato
    • Akane Abe
    • Keiko Haji
    • Ryohiko Ozaki
    • Hiromitsu Takizawa
    • Yasuhiko Nishioka
  • View Affiliations

  • Published online on: September 30, 2022     https://doi.org/10.3892/ol.2022.13537
  • Article Number: 417
  • Copyright: © Tobiume et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Fibrocytes, which are bone marrow‑derived collagen‑producing cells, have been reported to be involved in pathogenesis of pulmonary fibrosis. Our previous study reported that tumor‑infiltrating fibrocytes play a role in tumor progression and drug resistance in lung cancer. The present study therefore examined chemotactic factors for fibrocytes in tissues of non‑small cell lung cancer (NSCLC) and their prognostic significance. Surgically resected tumor tissues were examined for the expression of chemotactic factors, including C‑X‑C motif chemokine 12 (CXCL12), CCL2, platelet‑derived growth factor (PDGF)‑AA and PDGF‑BB, as well as tumor‑infiltrating fibrocytes by immunostaining. The chemotactic ability of fibrocytes in response to each factor was evaluated using a migration assay by counting the migrated cells microscopically, and expression of receptors for chemotactic factors were analyzed by flow cytometry. The expression of CXCL12, but not CCL2, PDGF‑AA, or PDGF‑BB, was associated with the number of tumor‑infiltrating fibrocytes in lung adenocarcinoma (LUAD), but not lung squamous cell carcinoma (LUSQ). In addition, patients with an increased expression of CXCL12 in LUAD but not LUSQ showed a significantly poorer prognosis compared with those with a decreased expression. However, the expression of CCL2, PDGF‑AA and PDGF‑BB was not correlated with the prognosis of patients with NSCLC. The number of fibrocytes was associated with a poor prognosis in LUAD. Fibrocytes derived from the peripheral blood of healthy subjects as well as patients with lung cancer expressed higher levels of CXCR4 compared with CCR2, PDGF and receptor‑α and receptor‑β. Overall, these results suggested that targeting tumor‑infiltrating fibrocytes via the CXCL12/CXCR4 axis may be a useful strategy for controlling the progression of NSCLC, particularly LUAD.
View Figures
View References

Related Articles

Journal Cover

November-2022
Volume 24 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Tobiume M, Mitsuhashi A, Saijo A, Ogino H, Afroj T, Ogawa H, Goto H, Sato S, Abe A, Haji K, Haji K, et al: Analysis of the chemotactic factors for tumor‑infiltrating fibrocytes and their prognostic significances in lung cancer. Oncol Lett 24: 417, 2022
APA
Tobiume, M., Mitsuhashi, A., Saijo, A., Ogino, H., Afroj, T., Ogawa, H. ... Nishioka, Y. (2022). Analysis of the chemotactic factors for tumor‑infiltrating fibrocytes and their prognostic significances in lung cancer. Oncology Letters, 24, 417. https://doi.org/10.3892/ol.2022.13537
MLA
Tobiume, M., Mitsuhashi, A., Saijo, A., Ogino, H., Afroj, T., Ogawa, H., Goto, H., Sato, S., Abe, A., Haji, K., Ozaki, R., Takizawa, H., Nishioka, Y."Analysis of the chemotactic factors for tumor‑infiltrating fibrocytes and their prognostic significances in lung cancer". Oncology Letters 24.5 (2022): 417.
Chicago
Tobiume, M., Mitsuhashi, A., Saijo, A., Ogino, H., Afroj, T., Ogawa, H., Goto, H., Sato, S., Abe, A., Haji, K., Ozaki, R., Takizawa, H., Nishioka, Y."Analysis of the chemotactic factors for tumor‑infiltrating fibrocytes and their prognostic significances in lung cancer". Oncology Letters 24, no. 5 (2022): 417. https://doi.org/10.3892/ol.2022.13537