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Article Open Access

Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer

  • Authors:
    • Cheng Ding
    • Zhangping Yu
    • Xianliang Li
    • Jiqiao Zhu
    • Menghua Dai
    • Qiang He
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Beijing Chao‑Yang Hospital Affiliated to Capital Medical University, Beijing 100020, P.R. China, Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 77
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    Published online on: January 5, 2023
       https://doi.org/10.3892/ol.2023.13663
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Abstract

Pancreatic cancer (PC) is a stubborn malignancy with high lethality and a low 5‑year overall survival (OS) rate. Collagen type VII α1 chain (COL7A1), a major component of the extracellular matrix, serves important roles in numerous physiological processes and various illnesses. COL7A1 protein acts as an anchoring fibril between the external epithelial cells and the underlying stroma, and mutation of COL7A1 could cause recessive dystrophic epidermolysis bullosa. Raw data for PC were acquired from The Cancer Genome Atlas and the Gene Expression Omnibus database, and raw data for the normal pancreas were obtained from the Genotype‑Tissue Expression database. COL7A1 mRNA expression in PC tissues was compared with that in either paired (GSE15471 dataset) or unpaired (all other data) normal pancreas tissues. The association between COL7A1 mRNA expression and clinicopathological factors was assessed using logistic regression analysis. Cox analysis and Kaplan‑Meier analysis were used to evaluate the role of COL7A1 mRNA expression in prognosis and nomograms were constructed. Gene Ontology analysis, Kyoto Encyclopedia of Genes and Genomes analysis, Gene Set Enrichment Analysis (GSEA) and single‑sample GSEA (ssGSEA) were performed to evaluate the relevant functions of COL7A1 and correlation with immune cell infiltration. Furthermore, reverse transcription‑quantitative PCR was used to assess the mRNA expression levels of COL7A1 in PC. The present study demonstrated that COL7A1 mRNA expression was higher in PC tissues compared with in normal pancreas tissues. The Kaplan‑Meier survival analysis indicated that patients with PC with high COL7A1 mRNA expression had shorter overall survival (OS), disease‑specific survival (DSS) and progression‑free interval (PFI) times compared with patients with PC with low COL7A1 mRNA expression. Multivariate analysis demonstrated that COL7A1 mRNA expression was an independent risk factor for OS, DSS and PFI. Nomogram and calibration plots were constructed to predict the prognosis of patients with PC. GSEA demonstrated that high mRNA expression levels of COL7A1 were associated with multiple cancer‑related pathways. ssGSEA analysis indicated that COL7A1 expression was positively associated with natural killer CD56bright cells and T helper (Th)2 cells, and negatively associated with Th17 cells and eosinophils. The results of the present study suggested that COL7A1 could be an independent biomarker and an influential moderator of immune infiltration in PC.
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Copy and paste a formatted citation
Spandidos Publications style
Ding C, Yu Z, Li X, Zhu J, Dai M and He Q: Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer. Oncol Lett 25: 77, 2023.
APA
Ding, C., Yu, Z., Li, X., Zhu, J., Dai, M., & He, Q. (2023). Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer. Oncology Letters, 25, 77. https://doi.org/10.3892/ol.2023.13663
MLA
Ding, C., Yu, Z., Li, X., Zhu, J., Dai, M., He, Q."Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer". Oncology Letters 25.2 (2023): 77.
Chicago
Ding, C., Yu, Z., Li, X., Zhu, J., Dai, M., He, Q."Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer". Oncology Letters 25, no. 2 (2023): 77. https://doi.org/10.3892/ol.2023.13663
Copy and paste a formatted citation
x
Spandidos Publications style
Ding C, Yu Z, Li X, Zhu J, Dai M and He Q: Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer. Oncol Lett 25: 77, 2023.
APA
Ding, C., Yu, Z., Li, X., Zhu, J., Dai, M., & He, Q. (2023). Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer. Oncology Letters, 25, 77. https://doi.org/10.3892/ol.2023.13663
MLA
Ding, C., Yu, Z., Li, X., Zhu, J., Dai, M., He, Q."Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer". Oncology Letters 25.2 (2023): 77.
Chicago
Ding, C., Yu, Z., Li, X., Zhu, J., Dai, M., He, Q."Collagen type VII α1 chain: A promising prognostic and immune infiltration biomarker of pancreatic cancer". Oncology Letters 25, no. 2 (2023): 77. https://doi.org/10.3892/ol.2023.13663
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